ID   HSP73_YEAST             Reviewed;         649 AA.
AC   P09435; D6VPS7; P18862;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 3.
DT   02-SEP-2026, entry version 218.
DE   RecName: Full=Heat shock protein SSA3;
GN   Name=SSA3; OrderedLocusNames=YBL075C; ORFNames=YBL06.07, YBL0610;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=8151709; DOI=10.1007/bf00175490;
RA   Boorstein W.R., Ziegelhoffer T., Craig E.A.;
RT   "Molecular evolution of the HSP70 multigene family.";
RL   J. Mol. Evol. 38:1-17(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-628.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7992509; DOI=10.1002/yea.320100811;
RA   Logghe M., Molemans F., Fiers W., Contreras R.;
RT   "The two genes encoding yeast ribosomal protein S8 reside on different
RT   chromosomes, and are closely linked to the hsp70 stress protein genes SSA3
RT   and SSA4.";
RL   Yeast 10:1093-1100(1994).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-205.
RX   PubMed=1922071; DOI=10.1128/mcb.11.11.5693-5700.1991;
RA   Nakazawa N., Harashima S., Oshima Y.;
RT   "AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type
RT   control of Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 11:5693-5700(1991).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
RX   PubMed=2188113; DOI=10.1128/mcb.10.6.3262-3267.1990;
RA   Boorstein W.R., Craig E.A.;
RT   "Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces
RT   cerevisiae.";
RL   Mol. Cell. Biol. 10:3262-3267(1990).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 602-649.
RC   STRAIN=ATCC 204626 / S288c / A364A;
RX   PubMed=2663194; DOI=10.1007/bf00435455;
RA   Martindale D.W., Gu Z.M., Csank C.;
RT   "Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase
RT   gene (ILS1).";
RL   Curr. Genet. 15:99-106(1989).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Present with 6440 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; M97225; AAC37398.1; -; Unassigned_DNA.
DR   EMBL; Z35836; CAA84896.1; -; Genomic_DNA.
DR   EMBL; Z26879; CAA81523.1; -; Genomic_DNA.
DR   EMBL; D90455; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; M36115; AAA35097.1; -; Genomic_DNA.
DR   EMBL; X07886; CAA30732.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BK006936; DAA07047.1; -; Genomic_DNA.
DR   PIR; S36753; S36753.
DR   RefSeq; NP_009478.1; NM_001178315.1.
DR   AlphaFoldDB; P09435; -.
DR   SMR; P09435; -.
DR   BioGRID; 32627; 161.
DR   DIP; DIP-2266N; -.
DR   FunCoup; P09435; 1430.
DR   IntAct; P09435; 62.
DR   MINT; P09435; -.
DR   STRING; 4932.YBL075C; -.
DR   GlyGen; P09435; 1 site.
DR   iPTMnet; P09435; -.
DR   PaxDb; 4932-YBL075C; -.
DR   PeptideAtlas; P09435; -.
DR   EnsemblFungi; YBL075C_mRNA; YBL075C; YBL075C.
DR   GeneID; 852203; -.
DR   KEGG; sce:YBL075C; -.
DR   AGR; SGD:S000000171; -.
DR   SGD; S000000171; SSA3.
DR   VEuPathDB; FungiDB:YBL075C; -.
DR   eggNOG; KOG0101; Eukaryota.
DR   HOGENOM; CLU_005965_3_0_1; -.
DR   InParanoid; P09435; -.
DR   OMA; VCKPIVT; -.
DR   OrthoDB; 2401965at2759; -.
DR   BioCyc; YEAST:G3O-28967-MONOMER; -.
DR   Reactome; R-SCE-3371453; Regulation of HSF1-mediated heat shock response.
DR   Reactome; R-SCE-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR   Reactome; R-SCE-3371568; Attenuation phase.
DR   Reactome; R-SCE-3371571; HSF1-dependent transactivation.
DR   Reactome; R-SCE-6798695; Neutrophil degranulation.
DR   Reactome; R-SCE-8876725; Protein methylation.
DR   BioGRID-ORCS; 852203; 1 hit in 10 CRISPR screens.
DR   CD-CODE; E03F929F; Stress granule.
DR   PRO; PR:P09435; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P09435; protein.
DR   GO; GO:0005829; C:cytosol; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IGI:SGD.
DR   GO; GO:0031072; F:heat shock protein binding; IBA:GO_Central.
DR   GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR   GO; GO:0006457; P:protein folding; IGI:SGD.
DR   GO; GO:0006515; P:protein quality control for misfolded or incompletely synthesized proteins; IMP:SGD.
DR   GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IMP:SGD.
DR   CDD; cd10233; ASKHA_NBD_HSP70_HSPA1; 1.
DR   FunFam; 2.60.34.10:FF:000002; Heat shock 70 kDa; 1.
DR   FunFam; 3.90.640.10:FF:000002; Heat shock 70 kDa; 1.
DR   FunFam; 3.30.420.40:FF:000172; Heat shock 70 kDa protein; 1.
DR   FunFam; 3.30.30.30:FF:000001; heat shock 70 kDa protein-like; 1.
DR   FunFam; 3.30.420.40:FF:000135; Heat shock cognate 71 kDa protein; 1.
DR   FunFam; 1.20.1270.10:FF:000021; Heat shock protein 70; 1.
DR   FunFam; 3.30.420.40:FF:000026; Heat shock protein 70; 1.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 3.30.30.30; -; 1.
DR   Gene3D; 3.30.420.40; -; 2.
DR   Gene3D; 3.90.640.10; Actin, Chain A, domain 4; 1.
DR   Gene3D; 2.60.34.10; Substrate Binding Domain Of DNAk, Chain A, domain 1; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   NCBIfam; NF001413; PRK00290.1; 1.
DR   PANTHER; PTHR19375; HEAT SHOCK PROTEIN 70KDA; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   PRINTS; PR00301; HEATSHOCK70.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR   SUPFAM; SSF100934; Heat shock protein 70kD (HSP70), C-terminal subdomain; 1.
DR   SUPFAM; SSF100920; Heat shock protein 70kD (HSP70), peptide-binding domain; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome;
KW   Stress response.
FT   CHAIN           1..649
FT                   /note="Heat shock protein SSA3"
FT                   /id="PRO_0000078387"
FT   REGION          608..649
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        611..626
FT                   /note="Gly residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        620
FT                   /note="G -> A (in Ref. 7; CAA30732)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   649 AA;  70547 MW;  F4B136759B7D4636 CRC64;
     MSRAVGIDLG TTYSCVAHFS NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAIN
     PHNTVFDAKR LIGRKFDDPE VTTDAKHFPF KVISRDGKPV VQVEYKGETK TFTPEEISSM
     VLSKMKETAE NYLGTTVNDA VVTVPAYFND SQRQATKDAG TIAGMNVLRI INEPTAAAIA
     YGLDKKGRAE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDNRLVNHLA
     TEFKRKTKKD ISNNQRSLRR LRTAAERAKR ALSSSSQTSI EIDSLFEGMD FYTSLTRARF
     EELCADLFRS TLEPVEKVLK DSKLDKSQID EIVLVGGSTR IPKIQKLVSD FFNGKEPNRS
     INPDEAVAYG AAVQAAILTG DQSTKTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT
     KKSETFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIDVTFDIDA
     NGILNVSALE KGTGKSNKIT ITNDKGRLSK DDIDRMVSEA EKYRADDERE AERVQAKNQL
     ESYAFTLKNT INEASFKEKV GEDDAKRLET ASQETIDWLD ASQAASTDEY KDRQKELEGI
     ANPIMTKFYG AGAGAGPGAG ESGGFPGSMP NSGATGGGED TGPTVEEVD
//