ID   T2D2_STREE              Reviewed;         288 AA.
AC   P09357;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   02-SEP-2026, entry version 102.
DE   RecName: Full=Type II restriction enzyme DpnII {ECO:0000303|PubMed:12654995};
DE            Short=R.DpnII;
DE            EC=3.1.21.4 {ECO:0000269|PubMed:2824782};
DE   AltName: Full=Endonuclease DpnII;
DE   AltName: Full=Type-2 restriction enzyme DpnII;
GN   Name=dpnB {ECO:0000303|PubMed:3019562};
OS   Streptococcus pneumoniae.
OC   Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1313;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3019562; DOI=10.1016/0092-8674(86)90698-7;
RA   Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.;
RT   "Genetic basis of the complementary DpnI and DpnII restriction systems of
RT   S. pneumoniae: an intercellular cassette mechanism.";
RL   Cell 46:993-1000(1986).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-11, FUNCTION, CATALYTIC ACTIVITY, AND SUBUNIT.
RX   PubMed=2824782; DOI=10.1016/0022-2836(87)90024-6;
RA   de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.;
RT   "Proteins encoded by the DpnII restriction gene cassette. Two methylases
RT   and an endonuclease.";
RL   J. Mol. Biol. 196:457-469(1987).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded unmethylated sequence 5'-GATC-3' and cleaves before G-1.
CC       {ECO:0000269|PubMed:2824782, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:2824782};
CC   -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:2824782}.
CC   -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; M14339; AAA88582.1; -; Genomic_DNA.
DR   PIR; B24372; B24372.
DR   RefSeq; WP_000815111.1; NZ_AP025938.1.
DR   AlphaFoldDB; P09357; -.
DR   REBASE; 777; DpnII.
DR   OMA; NFYPLHS; -.
DR   PRO; PR:P09357; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; EXP:UniProtKB.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR   InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR   InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR   Pfam; PF04556; DpnII; 1.
DR   PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   CHAIN           1..288
FT                   /note="Type II restriction enzyme DpnII"
FT                   /id="PRO_0000077302"
SQ   SEQUENCE   288 AA;  33585 MW;  F7346EEA6940EB33 CRC64;
     MKQTRNFDEW LSTMTDTVAD WTYYTDFPKV YKNVSSIKVA LNIMNSLIGS KNIQEDFLDL
     YQNYPEILKV VPLLIAKRLR DTIIVKDPIK DFYFDFSKRN YSIEEYTMFL EKSGIFDLLQ
     NHLVSNLVDY VTGVEVGMDT NGRKNRTGDA MENIVQSYLE AEGYILGENL FKEIEQNEIE
     EIFSVDLSAI TNDGNTVKRF DFVIKNEQVL YLIEVNFYSG SGSKLNETAR SYKMIAEETK
     AIPNVEFMWI TDGQGWYKAK NNLRETFDIL PFLYNINDLE HNILKNLK
//