ID OMPA_KLEAE Reviewed; 350 AA. AC P09146; DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1989, sequence version 1. DT 04-NOV-2026, entry version 123. DE RecName: Full=Outer membrane protein A {ECO:0000255|HAMAP-Rule:MF_00842}; DE AltName: Full=Outer membrane porin A {ECO:0000255|HAMAP-Rule:MF_00842}; DE Flags: Precursor; GN Name=ompA {ECO:0000255|HAMAP-Rule:MF_00842}; OS Klebsiella aerogenes (Enterobacter aerogenes). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; OC Enterobacterales; Enterobacteriaceae; Klebsiella. OX NCBI_TaxID=548; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=6363059; DOI=10.1111/j.1432-1033.1983.tb07853.x; RA Braun G., Cole S.T.; RT "Molecular characterization of the gene coding for major outer membrane RT protein OmpA from Enterobacter aerogenes."; RL Eur. J. Biochem. 137:495-500(1983). CC -!- FUNCTION: With TolR probably plays a role in maintaining the position CC of the peptidoglycan cell wall in the periplasm. Acts as a porin with CC low permeability that allows slow penetration of small solutes; an CC internal gate slows down solute passage. {ECO:0000255|HAMAP- CC Rule:MF_00842}. CC -!- FUNCTION: Required for conjugation with F-type plasmids; probably CC serves as the mating receptor on recipient cells. {ECO:0000255|HAMAP- CC Rule:MF_00842}. CC -!- SUBUNIT: Monomer and homodimer. {ECO:0000255|HAMAP-Rule:MF_00842}. CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP- CC Rule:MF_00842}; Multi-pass membrane protein {ECO:0000255|HAMAP- CC Rule:MF_00842}. CC -!- DOMAIN: The extracellular loops are most variable in sequence, and in CC some bacteria confer sensitivity to phage and/or colicins. CC {ECO:0000255|HAMAP-Rule:MF_00842}. CC -!- SIMILARITY: Belongs to the outer membrane OOP (TC 1.B.6) superfamily. CC OmpA family. {ECO:0000255|HAMAP-Rule:MF_00842}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X00254; CAA25062.1; -; Genomic_DNA. DR PIR; S07222; S07222. DR AlphaFoldDB; P09146; -. DR SMR; P09146; -. DR STRING; 548.EAG7_02416; -. DR PaxDb; 548-EAG7_02416; -. DR OMA; AAKHTNH; -. DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell. DR GO; GO:0015288; F:porin activity; IEA:UniProtKB-UniRule. DR GO; GO:0051701; P:biological process involved in interaction with host; IEA:UniProtKB-ARBA. DR GO; GO:0034220; P:monoatomic ion transmembrane transport; IEA:UniProtKB-UniRule. DR CDD; cd07185; OmpA_C-like; 1. DR FunFam; 3.30.1330.60:FF:000004; Outer membrane protein A; 1. DR Gene3D; 2.40.160.20; -; 1. DR Gene3D; 3.30.1330.60; OmpA-like domain; 1. DR HAMAP; MF_00842; OmpA; 1. DR InterPro; IPR050330; Bact_OuterMem_StrucFunc. DR InterPro; IPR011250; OMP/PagP_B-barrel. DR InterPro; IPR006664; OMP_bac. DR InterPro; IPR002368; OmpA. DR InterPro; IPR006665; OmpA-like. DR InterPro; IPR006690; OMPA-like_CS. DR InterPro; IPR036737; OmpA-like_sf. DR InterPro; IPR000498; OmpA-like_TM_dom. DR NCBIfam; NF008071; ompA; 1. DR PANTHER; PTHR30329:SF21; LIPOPROTEIN YIAD-RELATED; 1. DR PANTHER; PTHR30329; STATOR ELEMENT OF FLAGELLAR MOTOR COMPLEX; 1. DR Pfam; PF00691; OmpA; 1. DR Pfam; PF01389; OmpA_membrane; 1. DR PRINTS; PR01021; OMPADOMAIN. DR PRINTS; PR01022; OUTRMMBRANEA. DR SUPFAM; SSF56925; OMPA-like; 1. DR SUPFAM; SSF103088; OmpA-like; 1. DR PROSITE; PS01068; OMPA_1; 1. DR PROSITE; PS51123; OMPA_2; 1. PE 3: Inferred from homology; KW Cell outer membrane; Conjugation; Disulfide bond; Ion transport; Membrane; KW Porin; Repeat; Signal; Transmembrane; Transmembrane beta strand; Transport. FT SIGNAL 1..21 FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT CHAIN 22..350 FT /note="Outer membrane protein A" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT /id="PRO_0000020096" FT TRANSMEM 27..37 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 59..70 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 74..82 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 100..111 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 116..124 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 146..155 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 160..167 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT TRANSMEM 186..194 FT /note="Beta stranded" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT REPEAT 205..206 FT /note="1" FT REPEAT 207..208 FT /note="2" FT REPEAT 209..210 FT /note="3" FT REPEAT 211..212 FT /note="4" FT DOMAIN 214..342 FT /note="OmpA-like" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT REGION 205..212 FT /note="4 X 2 AA tandem repeats of A-P" FT SITE 77 FT /note="Part of salt bridge gating mechanism" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT SITE 163 FT /note="Part of salt bridge gating mechanism" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" FT DISULFID 315..327 FT /evidence="ECO:0000255|HAMAP-Rule:MF_00842" SQ SEQUENCE 350 AA; 37575 MW; 6276C6F2F21065DA CRC64; MKKTAIAIAV ALAGFATVAQ AAPKDNTWYA GGKLGWSQFH DTGWYNSNLN NNGPTHESQL GAGAFGGYQV NPYLGFEMGY DWLGRMPYKG VKVNGAFSSQ AVQLTAKLGY PITDDLDIYT RLGGMVWRAD SSNSIAGDNH DTGVSPVFAG GVEWAMTRDI ATRLEYQWVN NIGDAGTVGV RPDNGMLSVG VSYRFGQEDN APVVAPAPAP APEVTTKTFT LKSDVLFNFN KATLKPEGQQ ALDQLYTQLS NMDPKDGSAV VLGYTDRIGS EQYNQKLSEK RAQSVVDYLV AKGIPANKIS ARGMGESDPV TGNTCDNVKA RAALIDCLAP DRRVAIEVKG YKDVVTQPQA //