ID   GLU2_MAIZE              Reviewed;         223 AA.
AC   P04706; P02860; P08415;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   02-SEP-2026, entry version 151.
DE   RecName: Full=Glutelin-2;
DE   AltName: Full=27 kDa zein;
DE   AltName: Full=Alcohol-soluble reduced glutelin;
DE            Short=ASG;
DE   AltName: Full=Zein Zc2;
DE   AltName: Full=Zein-gamma;
DE   Flags: Precursor;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. W64 X W64O2;
RX   PubMed=3596247; DOI=10.1016/0378-1119(87)90393-3;
RA   Prat S., Perez-Grau L., Puigdomenech P.;
RT   "Multiple variability in the sequence of a family of maize endosperm
RT   proteins.";
RL   Gene 52:41-49(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. inbred line E-10;
RX   PubMed=3839076; DOI=10.1093/nar/13.5.1493;
RA   Prat S., Cortadas J., Puigdomenech P., Palau J.;
RT   "Nucleic acid (cDNA) and amino acid sequences of the maize endosperm
RT   protein glutelin-2.";
RL   Nucleic Acids Res. 13:1493-1504(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RA   Boronat A., Martinez M.C., Reina M., Puigdomenech P., Palau J.;
RT   "Isolation and sequencing of a 28 kd glutelin-2 gene from maize: common
RT   elements in the 5' flanking regions among zein and glutelin genes.";
RL   Plant Sci. 47:95-102(1986).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Wisconsin 64A; TISSUE=Endosperm;
RX   PubMed=2243788; DOI=10.1093/nar/18.21.6426;
RA   Reina M., Ponte I., Guillen P., Boronat A., Palau J.;
RT   "Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays
RT   W64 A.";
RL   Nucleic Acids Res. 18:6426-6426(1990).
RN   [5]
RP   NUCLEOTIDE SEQUENCE OF 15-223, AND VARIANT SER-28.
RX   PubMed=16664810; DOI=10.1104/pp.81.1.70;
RA   Wang S.-Z., Esen A.;
RT   "Primary structure of a proline-rich zein and its cDNA.";
RL   Plant Physiol. 81:70-74(1986).
RN   [6]
RP   PROTEIN SEQUENCE OF 20-77.
RA   Esen A., Bietz J.A., Paulis J.W., Wall J.S.;
RT   "Tandem repeats in the N-terminal sequence of a proline-rich protein from
RT   corn endosperm.";
RL   Nature 296:678-679(1982).
CC   -!- FUNCTION: Seed storage protein. It accounts for about 15% of the total
CC       endosperm protein content.
CC   -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain membrane. Note=Border of
CC       the inner part of the membrane of endosperm protein bodies.
CC   -!- SIMILARITY: Belongs to the gliadin/glutenin family. {ECO:0000305}.
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DR   EMBL; X02230; CAA26149.1; -; mRNA.
DR   EMBL; M16066; AAA33468.1; -; Genomic_DNA.
DR   EMBL; M16218; AAA33537.1; -; mRNA.
DR   EMBL; X53514; CAA37594.1; -; Genomic_DNA.
DR   EMBL; S78780; AAP32017.1; -; mRNA.
DR   PIR; A03351; ZMZM5.
DR   PIR; A93557; ZMZM19.
DR   RefSeq; NP_001337252.1; NM_001350323.1.
DR   AlphaFoldDB; P04706; -.
DR   FunCoup; P04706; 3.
DR   STRING; 4577.P04706; -.
DR   Allergome; 2780; Zea m 27kD Zein.
DR   PaxDb; 4577-P04706; -.
DR   EnsemblPlants; Zm00001eb313800_T001; Zm00001eb313800_P001; Zm00001eb313800.
DR   GeneID; 542296; -.
DR   Gramene; Zm00001eb313800_T001; Zm00001eb313800_P001; Zm00001eb313800.
DR   KEGG; zma:542296; -.
DR   MaizeGDB; 58111; -.
DR   eggNOG; ENOG502R4K8; Eukaryota.
DR   HOGENOM; CLU_1477240_0_0_1; -.
DR   InParanoid; P04706; -.
DR   OMA; NDCCCGC; -.
DR   Proteomes; UP000007305; Chromosome 7.
DR   ExpressionAtlas; P04706; baseline and differential.
DR   GO; GO:0032578; C:aleurone grain membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:InterPro.
DR   Gene3D; 1.10.110.10; Plant lipid-transfer and hydrophobic proteins; 1.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR001954; Glia_glutenin.
DR   InterPro; IPR000480; Glutelin.
DR   PANTHER; PTHR33454:SF17; GLUTELIN-2; 1.
DR   PANTHER; PTHR33454; PROLAMIN PPROL 14P; 1.
DR   Pfam; PF13016; Gliadin; 1.
DR   PRINTS; PR00211; GLUTELIN.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Membrane; Reference proteome; Repeat;
KW   Seed storage protein; Signal; Storage protein; Vacuole.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|Ref.6"
FT   CHAIN           20..223
FT                   /note="Glutelin-2"
FT                   /id="PRO_0000032214"
FT   REPEAT          31..36
FT                   /note="1-1"
FT   REPEAT          37..42
FT                   /note="1-2"
FT   REPEAT          43..48
FT                   /note="1-3"
FT   REPEAT          49..54
FT                   /note="1-4"
FT   REPEAT          55..60
FT                   /note="1-5"
FT   REPEAT          61..66
FT                   /note="1-6"
FT   REPEAT          67..72
FT                   /note="1-7"
FT   REPEAT          73..78
FT                   /note="1-8"
FT   REPEAT          97..104
FT                   /note="2-1"
FT   REPEAT          105..112
FT                   /note="2-2"
FT   REGION          31..78
FT                   /note="8 X 6 AA tandem repeats of P-P-P-V-H-L"
FT   REGION          97..112
FT                   /note="2 X 8 AA tandem repeats of Q-P-H-P-C-P-C-Q"
FT   VARIANT         28
FT                   /note="C -> S (in 66% of the molecules)"
FT                   /evidence="ECO:0000269|PubMed:16664810"
SQ   SEQUENCE   223 AA;  23689 MW;  3E80C06B2DB58372 CRC64;
     MRVLLVALAL LALAASATST HTSGGCGCQP PPPVHLPPPV HLPPPVHLPP PVHLPPPVHL
     PPPVHLPPPV HVPPPVHLPP PPCHYPTQPP RPQPHPQPHP CPCQQPHPSP CQLQGTCGVG
     STPILGQCVE FLRHQCSPTA TPYCSPQCQS LRQQCCQQLR QVEPQHRYQA IFGLVLQSIL
     QQQPQSGQVA GLLAAQIAQQ LTAMCGLQQP TPCPYAAAGG VPH
//