ID SIGNS_REOVD Reviewed; 366 AA. AC P03526; A4ZY28; Q9ENN1; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 21-JUL-1986, sequence version 1. DT 02-SEP-2026, entry version 89. DE RecName: Full=Protein sigma-NS; DE Short=SigmaNS; GN Name=S3; OS Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3). OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; OC Reovirales; Spinareoviridae; Orthoreovirus; Orthoreovirus mammalis; OC Mammalian orthoreovirus. OX NCBI_TaxID=10886; OH NCBI_TaxID=40674; Mammalia. RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RX PubMed=6312421; DOI=10.1093/nar/11.18.6399; RA Richardson M.A., Furuichi Y.; RT "Nucleotide sequence of reovirus genome segment S3, encoding non-structural RT protein sigma NS."; RL Nucleic Acids Res. 11:6399-6408(1983). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION. RC STRAIN=Mutant tsE320; RX PubMed=11152519; DOI=10.1128/jvi.75.3.1459-1475.2001; RA Becker M.M., Goral M.I., Hazelton P.R., Baer G.S., Rodgers S.E., RA Brown E.G., Coombs K.M., Dermody T.S.; RT "Reovirus sigmaNS protein is required for nucleation of viral assembly RT complexes and formation of viral inclusions."; RL J. Virol. 75:1459-1475(2001). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Infectious clone; RX PubMed=18005692; DOI=10.1016/j.chom.2007.03.003; RA Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., RA Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., RA Wilson G.J., Chappell J.D., Dermody T.S.; RT "A plasmid-based reverse genetics system for animal double-stranded RNA RT viruses."; RL Cell Host Microbe 1:147-157(2007). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-17. RX PubMed=6927854; DOI=10.1016/0042-6822(82)90170-2; RA Antczak J.B., Chmelo R.A., Pickup D.J., Joklik W.K.; RT "Sequence at both termini of the 10 genes of reovirus serotype 3 (strain RT Dearing)."; RL Virology 121:307-319(1982). RN [5] RP SUBUNIT, AND INTERACTION WITH PROTEIN MU-NS. RX PubMed=12719587; DOI=10.1128/jvi.77.10.5948-5963.2003; RA Becker M.M., Peters T.R., Dermody T.S.; RT "Reovirus sigma NS and mu NS proteins form cytoplasmic inclusion structures RT in the absence of viral infection."; RL J. Virol. 77:5948-5963(2003). RN [6] RP FUNCTION, INTERACTION WITH HOST G3BP1, INTERACTION WITH PROTEIN MU-NS, AND RP SUBCELLULAR LOCATION. RX PubMed=28794026; DOI=10.1128/jvi.01298-17; RA Choudhury P., Bussiere L.D., Miller C.L.; RT "Mammalian Orthoreovirus Factories Modulate Stress Granule Protein RT Localization by Interaction with G3BP1."; RL J. Virol. 91:0-0(2017). CC -!- FUNCTION: Protein that binds to ssRNA and participates with protein mu- CC NS in forming the matrix of viral factories, which are large inclusions CC in the host cytoplasm where replication intermediates are assembled and CC viral RNA replication takes place (PubMed:11152519). Plays a role in CC the inhibition of the integrated stress response (ISR) to escape from CC host cell translational shutoff (PubMed:28794026). Participates in the CC disruption of stress granules (SG) through its association with host CC G3BP1 and mu-NS (PubMed:28794026). {ECO:0000269|PubMed:11152519, CC ECO:0000269|PubMed:28794026}. CC -!- SUBUNIT: Homooligomer; in presence of RNA (PubMed:12719587). Interacts CC with protein mu-NS; this interaction allows the localization of sigma- CC NS to the viral factories (PubMed:12719587, PubMed:28794026). Interacts CC with host G3BP1 (via C-terminus); this interaction induces the CC relocalization of G3BP1 and other SG proteins to the viral factories CC periphery (PubMed:28794026). {ECO:0000269|PubMed:12719587, CC ECO:0000269|PubMed:28794026}. CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:P12002}. CC Note=Localizes to the viral factories formed by mu-NS. CC {ECO:0000250|UniProtKB:P12002}. CC -!- MISCELLANEOUS: There is a strain variablity in the inhibition of the CC host integrated stress response (ISR). There is a correlation with the CC abilities of the strains to prevent upstream PKR activation and CC EIF2S1/eIF2-alpha phosphorylation. {ECO:0000269|PubMed:28794026}. CC -!- SIMILARITY: Belongs to the orthoreovirus sigma-NS protein family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X01627; CAA25768.1; -; Genomic_RNA. DR EMBL; AF076293; AAG02124.1; -; mRNA. DR EMBL; EF494443; ABP48921.1; -; Genomic_RNA. DR EMBL; J02330; AAA47280.1; -; Genomic_RNA. DR PIR; A04126; MNXRSD. DR PIR; C27401; C27401. DR RefSeq; YP_010839449.1; NC_077837.1. DR PDB; 8TKA; X-ray; 3.00 A; A/B=1-366. DR PDB; 8TL1; X-ray; 3.16 A; A=18-366. DR PDB; 8TL8; X-ray; 3.20 A; A/B=1-366. DR SMR; P03526; -. DR GeneID; 80549138; -. DR KEGG; vg:80549138; -. DR GO; GO:0030430; C:host cell cytoplasm; ISS:UniProtKB. DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro. DR GO; GO:0003727; F:single-stranded RNA binding; IEA:InterPro. DR InterPro; IPR002507; Reovirus_polyG_pol. DR Pfam; PF01518; PolyG_pol; 1. DR PDBsum; 8TKA; -. DR PDBsum; 8TL1; -. DR PDBsum; 8TL8; -. PE 1: Evidence at protein level; KW 3D-structure; Host cytoplasm; RNA-binding. FT CHAIN 1..366 FT /note="Protein sigma-NS" FT /id="PRO_0000222759" FT REGION 1..11 FT /note="Important for ssRNA-binding and formation of FT complexes" FT /evidence="ECO:0000250|UniProtKB:P07940" FT VARIANT 260 FT /note="M -> T (in strain: Mutant tsE320)" FT HELIX 4..11 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 17..22 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 26..28 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 30..37 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 40..46 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 48..54 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 55..57 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 59..63 FT /evidence="ECO:0007829|PDB:8TKA" FT TURN 64..68 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 72..79 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 85..90 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 96..98 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 105..116 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 120..126 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 135..146 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 149..151 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 153..155 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 157..159 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 161..163 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 164..169 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 174..183 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 186..190 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 197..200 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 202..209 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 210..215 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 236..239 FT /evidence="ECO:0007829|PDB:8TKA" FT TURN 242..244 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 245..271 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 273..276 FT /evidence="ECO:0007829|PDB:8TKA" FT TURN 277..279 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 283..296 FT /evidence="ECO:0007829|PDB:8TKA" FT TURN 297..299 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 301..312 FT /evidence="ECO:0007829|PDB:8TKA" FT TURN 313..315 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 319..329 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 332..334 FT /evidence="ECO:0007829|PDB:8TKA" FT HELIX 337..339 FT /evidence="ECO:0007829|PDB:8TL1" FT STRAND 341..344 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 349..353 FT /evidence="ECO:0007829|PDB:8TKA" FT STRAND 356..361 FT /evidence="ECO:0007829|PDB:8TKA" SQ SEQUENCE 366 AA; 41057 MW; 201A6223C4290D7D CRC64; MASSLRAAIS KIKRDDVGQQ VCPNYVMLRS SVTTKVVRNV VEYQIRTGGF FSCLAMLRPL QYAKRERLLG QRNLERISTR DILQTRDLHS LCMPTPDAPM SNHQASTMRE LICSYFKVDH ADGLKYIPMD ERYSPSSLAR LFTMGMAGLH ITTEPSYKRV PIMHLAADLD CMTLALPYMI TLDGDTVVPV APTLSAEQLL DDGLKGLACM DISYGCEVDA NSRPAGDQSM DSSRCINELY CEETAEAICV LKTCLVLNCM QFKLEMDDLA HNAAELDKIQ MMIPFSERVF RMASSFATID AQCFRFCVMM KDKNLKIDMR ETTRLWTRSA SDDSVATSSL SISLDRGRWV AADASDARLL VFPIRV //