ID MONA_DIOCU Reviewed; 45 AA. AC P02881; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 21-JUL-1986, sequence version 1. DT 04-NOV-2026, entry version 123. DE RecName: Full=Monellin chain A; DE AltName: Full=Monellin chain I; OS Dioscoreophyllum cumminsii (Serendipity berry) (Rhopalandria cumminsii). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Ranunculales; Menispermaceae; OC Chasmantheroideae; Burasaieae; Dioscoreophyllum. OX NCBI_TaxID=3457; RN [1] RP PROTEIN SEQUENCE. RX PubMed=1368575; DOI=10.1271/bbb1961.54.2219; RA Kohmura M., Nio N., Ariyoshi Y.; RT "Complete amino acid sequence of the sweet protein monellin."; RL Agric. Biol. Chem. 54:2219-2224(1990). RN [2] RP PROTEIN SEQUENCE. RX PubMed=964918; DOI=10.1515/bchm2.1976.357.1.585; RA Frank G., Zuber H.; RT "The complete amino acid sequences of both subunits of the sweet protein RT monellin."; RL Hoppe-Seyler's Z. Physiol. Chem. 357:585-592(1976). RN [3] RP PROTEIN SEQUENCE. RX PubMed=962914; DOI=10.1016/0006-291x(76)90270-9; RA Hudson G., Biemann K.; RT "Mass spectrometric sequencing of proteins. The structure of subunit I of RT monellin."; RL Biochem. Biophys. Res. Commun. 71:212-220(1976). RN [4] RP PROTEIN SEQUENCE OF 2-28. RX PubMed=4107; DOI=10.1016/0005-2795(76)90293-2; RA Bohak Z., Li S.-L.; RT "The structure of monellin and its relation to the sweetness of the RT protein."; RL Biochim. Biophys. Acta 427:153-170(1976). RN [5] RP X-RAY CRYSTALLOGRAPHY. RX PubMed=3614382; DOI=10.1038/328739a0; RA Ogata C., Hatada M., Tomlinson G., Shin W.-C., Kim S.-H.; RT "Crystal structure of the intensely sweet protein monellin."; RL Nature 328:739-742(1987). RN [6] RP X-RAY CRYSTALLOGRAPHY. RX PubMed=15299859; DOI=10.1107/s0907444997006860; RA Bujacz G., Miller M., Harrison R., Thanki N., Gilliland G.L., Ogata C., RA Kim S.-H., Wlodawer A.; RT "Structure of monellin refined to 2.3-A resolution in the orthorhombic RT crystal form."; RL Acta Crystallogr. D 53:713-719(1997). RN [7] RP SIMILARITY TO CYSTATINS. RX PubMed=8464079; DOI=10.1006/jmbi.1993.1186; RA Murzin A.G.; RT "Sweet-tasting protein monellin is related to the cystatin family of thiol RT proteinase inhibitors."; RL J. Mol. Biol. 230:689-694(1993). CC -!- FUNCTION: Taste-modifying protein; intensely sweet-tasting protein. CC -!- SUBUNIT: Heterodimer of an A chain and a B chain. CC -!- BIOTECHNOLOGY: Natural monellin has not been mass marketed to the food CC industry, primarily due to the challenge of large-scale purification of CC the protein which is relatively sensitive to heat or acid treatment. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR PIR; JH0209; MLDIA. DR PDB; 1FA3; NMR; -; A=1-45. DR PDB; 1FUW; NMR; -; A=2-42. DR PDB; 1IV7; X-ray; 1.82 A; A/B=1-45. DR PDB; 1IV9; X-ray; 1.90 A; A/B=1-45. DR PDB; 1KRL; X-ray; 1.90 A; A/C=2-45. DR PDB; 1M9G; NMR; -; A=1-45. DR PDB; 1MNL; NMR; -; A=1-45. DR PDB; 3MON; X-ray; 2.80 A; A/C/E/G=2-45. DR PDB; 3PXM; X-ray; 1.80 A; A/B=2-45. DR PDB; 3PYJ; X-ray; 2.00 A; A=2-45. DR PDB; 3Q2P; X-ray; 2.34 A; A/B/C/D=2-45. DR PDB; 4MON; X-ray; 2.30 A; A/C=1-45. DR PDB; 5LC6; X-ray; 1.70 A; A/B=1-45. DR PDB; 5LC7; X-ray; 1.55 A; A/B=1-45. DR PDB; 5O7K; X-ray; 2.05 A; A/B=1-45. DR PDB; 5O7Q; X-ray; 1.72 A; A/B=1-45. DR PDB; 5O7R; X-ray; 2.06 A; A/B=1-45. DR PDB; 5O7S; X-ray; 2.02 A; A/B=1-45. DR PDB; 5XFU; X-ray; 2.61 A; A/B/C/D/E=4-45. DR PDB; 5YCT; X-ray; 1.85 A; A/B=1-45. DR PDB; 5YCU; X-ray; 2.32 A; A/B/C/D/E=4-45. DR PDB; 5YCW; X-ray; 2.29 A; A=4-45. DR PDB; 5Z1P; X-ray; 1.89 A; A/B/C/D=1-45. DR PDB; 6L44; X-ray; 2.49 A; A/B=6-45. DR PDB; 6L4I; X-ray; 2.20 A; A/B=4-45. DR PDB; 6L4J; X-ray; 2.30 A; A/B=4-45. DR PDB; 6L4N; X-ray; 2.43 A; A/B=6-45. DR PDB; 6LAY; X-ray; 3.00 A; A/B=6-45. DR PDB; 7D75; X-ray; 2.50 A; A/B/C/D=5-45. DR PDB; 7EUA; X-ray; 2.43 A; A=1-45. DR PDB; 7VWW; X-ray; 2.70 A; A/B/C/D/E/F/G/H=4-45. DR PDB; 8JZ0; X-ray; 1.23 A; A/B=2-45. DR PDB; 8JZ1; X-ray; 1.24 A; A/B=2-45. DR PDB; 8Q0R; X-ray; 1.55 A; AAA/BBB=1-45. DR PDB; 8Q0S; X-ray; 1.19 A; AAA/BBB=1-45. DR PDB; 8TM8; X-ray; 2.26 A; A/B=4-45. DR PDB; 8ZXJ; X-ray; 1.50 A; A/B=1-45. DR PDB; 8ZXT; X-ray; 1.90 A; A/B=1-45. DR PDB; 8ZXV; X-ray; 1.43 A; A/B=1-45. DR PDB; 8ZXY; X-ray; 1.43 A; A/B=1-45. DR PDB; 9HIJ; X-ray; 1.60 A; A/B=1-45. DR PDB; 9HIK; X-ray; 1.15 A; A=1-45. DR PDB; 9HKG; X-ray; 1.26 A; A=1-45. DR PDB; 9MYK; X-ray; 2.87 A; A/B/C/D/E=5-45. DR SMR; P02881; -. DR IntAct; P02881; 1. DR EvolutionaryTrace; P02881; -. DR Gene3D; 6.20.50.130; -; 1. DR InterPro; IPR046350; Cystatin_sf. DR InterPro; IPR015283; Monellin. DR InterPro; IPR000828; Monellin_A. DR InterPro; IPR038580; Monellin_A_sf. DR Pfam; PF09200; Monellin; 1. DR PRINTS; PR00630; MONELLINA. DR SUPFAM; SSF54403; Cystatin/monellin; 1. DR AlphaFoldDB; P02881; -. PE 1: Evidence at protein level; KW 3D-structure; Direct protein sequencing; Taste-modifying protein. FT CHAIN 1..45 FT /note="Monellin chain A" FT /id="PRO_0000207165" FT VARIANT 1 FT /note="Missing (in 90% of the chains)" FT CONFLICT 23 FT /note="D -> N (in Ref. 3; AA sequence)" FT /evidence="ECO:0000305" FT CONFLICT 26..27 FT /note="ED -> QN (in Ref. 3; AA sequence)" FT /evidence="ECO:0000305" FT STRAND 1..3 FT /evidence="ECO:0007829|PDB:1M9G" FT STRAND 6..15 FT /evidence="ECO:0007829|PDB:9HIK" FT STRAND 18..27 FT /evidence="ECO:0007829|PDB:9HIK" FT TURN 28..30 FT /evidence="ECO:0007829|PDB:9HIK" FT STRAND 33..39 FT /evidence="ECO:0007829|PDB:9HIK" SQ SEQUENCE 45 AA; 5398 MW; E086DF18521EC5B6 CRC64; FREIKGYEYQ LYVYASDKLF RADISEDYKT RGRKLLRFNG PVPPP //