ID   PP343_ARATH             Reviewed;         624 AA.
AC   O65543; F4JR67;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   04-NOV-2026, entry version 144.
DE   RecName: Full=Pentatricopeptide repeat-containing protein At4g31070, mitochondrial;
DE   Flags: Precursor;
GN   Name=PCMP-E7; OrderedLocusNames=At4g31070; ORFNames=F6I18.20;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY.
RX   PubMed=10809006; DOI=10.1023/a:1006352315928;
RA   Aubourg S., Boudet N., Kreis M., Lecharny A.;
RT   "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family
RT   unique to plants.";
RL   Plant Mol. Biol. 42:603-613(2000).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA   Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA   Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA   Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA   Taconnat L., Small I.;
RT   "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT   reveals their essential role in organelle biogenesis.";
RL   Plant Cell 16:2089-2103(2004).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PPR family. PCMP-E subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA18186.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CAB79825.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC       URL="https://ppr.plantenergy.uwa.edu.au";
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL022198; CAA18186.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL161578; CAB79825.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002687; AEE85853.2; -; Genomic_DNA.
DR   EMBL; CP002687; ANM67009.1; -; Genomic_DNA.
DR   EMBL; OZ408686; CAO7049427.1; -; Genomic_DNA.
DR   PIR; H85363; H85363.
DR   RefSeq; NP_001320098.1; NM_001342052.1.
DR   RefSeq; NP_001328866.1; NM_001342053.1.
DR   AlphaFoldDB; O65543; -.
DR   SMR; O65543; -.
DR   FunCoup; O65543; 108.
DR   STRING; 3702.O65543; -.
DR   PaxDb; 3702-O65543; -.
DR   ProteomicsDB; 249241; -.
DR   GeneID; 829234; -.
DR   KEGG; ath:AT4G31070; -.
DR   Araport; AT4G31070; -.
DR   TAIR; AT4G31070; -.
DR   eggNOG; KOG4197; Eukaryota.
DR   HOGENOM; CLU_002706_15_10_1; -.
DR   InParanoid; O65543; -.
DR   OMA; AALMHMY; -.
DR   PhylomeDB; O65543; -.
DR   PRO; PR:O65543; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   Proteomes; UP001984100; Chromosome 4.
DR   GO; GO:0005739; C:mitochondrion; ISM:TAIR.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0009451; P:RNA modification; IBA:GO_Central.
DR   FunFam; 1.25.40.10:FF:000090; Pentatricopeptide repeat-containing protein, chloroplastic; 1.
DR   Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 4.
DR   InterPro; IPR046848; E_motif.
DR   InterPro; IPR046960; PPR_At4g14850-like_plant.
DR   InterPro; IPR002885; PPR_rpt.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   NCBIfam; TIGR00756; PPR; 4.
DR   PANTHER; PTHR47926:SF544; PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR47926; PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN; 1.
DR   Pfam; PF20431; E_motif; 1.
DR   Pfam; PF01535; PPR; 2.
DR   Pfam; PF12854; PPR_1; 1.
DR   Pfam; PF13041; PPR_2; 2.
DR   SUPFAM; SSF48452; TPR-like; 1.
DR   PROSITE; PS51375; PPR; 12.
PE   3: Inferred from homology;
KW   Mitochondrion; Reference proteome; Repeat; Transit peptide.
FT   TRANSIT         1..15
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..624
FT                   /note="Pentatricopeptide repeat-containing protein
FT                   At4g31070, mitochondrial"
FT                   /id="PRO_0000363460"
FT   REPEAT          56..91
FT                   /note="PPR 1"
FT   REPEAT          92..122
FT                   /note="PPR 2"
FT   REPEAT          123..157
FT                   /note="PPR 3"
FT   REPEAT          158..192
FT                   /note="PPR 4"
FT   REPEAT          195..225
FT                   /note="PPR 5"
FT   REPEAT          226..260
FT                   /note="PPR 6"
FT   REPEAT          261..296
FT                   /note="PPR 7"
FT   REPEAT          297..327
FT                   /note="PPR 8"
FT   REPEAT          328..362
FT                   /note="PPR 9"
FT   REPEAT          363..397
FT                   /note="PPR 10"
FT   REPEAT          398..428
FT                   /note="PPR 11"
FT   REPEAT          429..463
FT                   /note="PPR 12"
FT   REPEAT          464..498
FT                   /note="PPR 13"
FT   REPEAT          499..529
FT                   /note="PPR 14"
FT   REGION          534..610
FT                   /note="Type E motif"
SQ   SEQUENCE   624 AA;  69841 MW;  BC9BD98ED8CCE20D CRC64;
     MRWVKLGRRV IMSRALSSRL NLELGNKLKG LVSDQFYDEA LRLYKLKIHS LGTNGFTAIL
     PSVIKACAFQ QEPFLLGAQL HCLCLKAGAD CDTVVSNSLI SMYAKFSRKY AVRKVFDEML
     HRDTVSYCSI INSCCQDGLL YEAMKLIKEM YFYGFIPKSE LVASLLALCT RMGSSSKVAR
     MFHALVLVDE RMQESVLLST ALVDMYLKFD DHAAAFHVFD QMEVKNEVSW TAMISGCVAN
     QNYEMGVDLF RAMQRENLRP NRVTLLSVLP ACVELNYGSS LVKEIHGFSF RHGCHADERL
     TAAFMTMYCR CGNVSLSRVL FETSKVRDVV MWSSMISGYA ETGDCSEVMN LLNQMRKEGI
     EANSVTLLAI VSACTNSTLL SFASTVHSQI LKCGFMSHIL LGNALIDMYA KCGSLSAARE
     VFYELTEKDL VSWSSMINAY GLHGHGSEAL EIFKGMIKGG HEVDDMAFLA ILSACNHAGL
     VEEAQTIFTQ AGKYHMPVTL EHYACYINLL GRFGKIDDAF EVTINMPMKP SARIWSSLLS
     ACETHGRLDV AGKIIANELM KSEPDNPANY VLLSKIHTES GNYHAAEEVR RVMQRRKLNK
     CYGFSKIEPE LQIEDYQGKS WSPI
//