ID   VORA_PYRHO              Reviewed;         394 AA.
AC   O58413;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   02-SEP-2026, entry version 125.
DE   RecName: Full=Ketoisovalerate oxidoreductase subunit VorA;
DE            Short=VOR;
DE            EC=1.2.7.7;
DE   AltName: Full=2-oxoisovalerate ferredoxin reductase subunit alpha;
DE   AltName: Full=2-oxoisovalerate oxidoreductase alpha chain;
GN   Name=vorA; OrderedLocusNames=PH0680;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Methanobacteriati; Methanobacteriota; Thermococci; Thermococcales;
OC   Thermococcaceae; Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-methyl-2-oxobutanoate + 2 oxidized [2Fe-2S]-[ferredoxin] +
CC         CoA = 2-methylpropanoyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 +
CC         H(+); Xref=Rhea:RHEA:11712, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:11851, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57338; EC=1.2.7.7;
CC   -!- SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma
CC       chain. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA29771.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000001; BAA29771.1; ALT_INIT; Genomic_DNA.
DR   PIR; A71114; A71114.
DR   RefSeq; WP_048053189.1; NC_000961.1.
DR   AlphaFoldDB; O58413; -.
DR   SMR; O58413; -.
DR   IntAct; O58413; 1.
DR   MINT; O58413; -.
DR   STRING; 70601.gene:9377625; -.
DR   EnsemblBacteria; BAA29771; BAA29771; BAA29771.
DR   GeneID; 1443008; -.
DR   KEGG; pho:PH0680; -.
DR   eggNOG; arCOG01608; Archaea.
DR   OrthoDB; 372068at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0043807; F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006979; P:response to oxidative stress; IEA:TreeGrafter.
DR   GO; GO:0044272; P:sulfur compound biosynthetic process; IEA:UniProtKB-ARBA.
DR   CDD; cd07034; TPP_PYR_PFOR_IOR-alpha_like; 1.
DR   FunFam; 3.40.50.920:FF:000010; Pyruvate ferredoxin oxidoreductase, alpha subunit; 1.
DR   FunFam; 3.40.50.970:FF:000012; Pyruvate:ferredoxin (Flavodoxin) oxidoreductase; 1.
DR   Gene3D; 3.40.50.920; -; 1.
DR   Gene3D; 3.40.50.970; -; 1.
DR   InterPro; IPR033412; PFOR_II.
DR   InterPro; IPR050722; Pyruvate:ferred/Flavod_OxRd.
DR   InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR009014; Transketo_C/PFOR_II.
DR   NCBIfam; NF006232; PRK08366.1; 1.
DR   PANTHER; PTHR32154; PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED; 1.
DR   PANTHER; PTHR32154:SF0; PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED; 1.
DR   Pfam; PF17147; PFOR_II; 1.
DR   Pfam; PF01855; POR_N; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 1.
DR   SUPFAM; SSF52922; TK C-terminal domain-like; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..394
FT                   /note="Ketoisovalerate oxidoreductase subunit VorA"
FT                   /id="PRO_0000099953"
SQ   SEQUENCE   394 AA;  44126 MW;  68E5038B32D63FD3 CRC64;
     MEYNPIKKVV SGNYAAAYAA LHARVQVVAA YPITPQTSII EKIAEFIANG EADIQYIPVE
     SEHSAMAACI GASATGARVF TATSAQGLAL MHEMLHWAAG SRLPIVMVNV NRAMAPPWSV
     WDDQTDSLSQ RDTGWMQFYA ENNQEVYDGV LMAYKVAETV NIPTMIIESA FILSHTYEVV
     NMIPQELVDE FLPPRKPLYT LTDFENPIAV GALATPADYY EFRYKIAKAH EEAKKVIKSV
     GKEFGERFGR DYSKMIETGY IEDADFVFMG MGSLMGTVKE AVDLLRKEGY KVGYAKVRWF
     RPFPKEELLE IAESVKGIAV LDRNFSFGQE GILFTEAKGA LYNTGVRPIM KDYIVGLGGR
     DFTVRDVKAI AEDMKKVVES GKLDKEIEWY HLKR
//