ID   GUN7_ARATH              Reviewed;         623 AA.
AC   O04478;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   02-SEP-2026, entry version 145.
DE   RecName: Full=Endoglucanase 7;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 7;
GN   Name=KOR2; OrderedLocusNames=At1g65610; ORFNames=F5I14.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=11488474; DOI=10.1023/a:1010688726755;
RA   Moelhoej M., Joergensen B., Ulvskov P., Borkhardt B.;
RT   "Two Arabidopsis thaliana genes, KOR2 and KOR3, which encode membrane-
RT   anchored endo-1,4-beta-D-glucanases, are differentially expressed in
RT   developing leaf trichomes and their support cells.";
RL   Plant Mol. Biol. 46:263-275(2001).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in basal region of leaf blade and
CC       proximal parts of leaf and floral organ. {ECO:0000269|PubMed:11488474}.
CC   -!- DEVELOPMENTAL STAGE: Early expressed in the development of root hairs
CC       within the root differentiation zone. Expressed late in the development
CC       of leaf trichomes when the stalks and branches are expanded.
CC       {ECO:0000269|PubMed:11488474}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AC001229; AAB60922.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE34400.1; -; Genomic_DNA.
DR   EMBL; OZ408683; CAO7032197.1; -; Genomic_DNA.
DR   EMBL; AK229402; BAF01264.1; -; mRNA.
DR   PIR; B96681; B96681.
DR   RefSeq; NP_176738.1; NM_105234.3.
DR   AlphaFoldDB; O04478; -.
DR   SMR; O04478; -.
DR   FunCoup; O04478; 170.
DR   STRING; 3702.O04478; -.
DR   GlyCosmos; O04478; 9 sites, No reported glycans.
DR   GlyGen; O04478; 10 sites.
DR   PaxDb; 3702-O04478; -.
DR   ProteomicsDB; 247151; -.
DR   GeneID; 842872; -.
DR   KEGG; ath:AT1G65610; -.
DR   Araport; AT1G65610; -.
DR   TAIR; AT1G65610; KOR2.
DR   eggNOG; ENOG502QSIM; Eukaryota.
DR   HOGENOM; CLU_008926_1_3_1; -.
DR   InParanoid; O04478; -.
DR   OMA; MCSYLHQ; -.
DR   PhylomeDB; O04478; -.
DR   BioCyc; ARA:AT1G65610-MONOMER; -.
DR   PRO; PR:O04478; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; O04478; baseline and differential.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISM:TAIR.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase; Membrane;
KW   Polysaccharide degradation; Reference proteome; Signal-anchor;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..623
FT                   /note="Endoglucanase 7"
FT                   /id="PRO_0000249260"
FT   TOPO_DOM        1..79
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        80..100
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        101..623
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        517
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        565
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        574
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CARBOHYD        116
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        221
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        328
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        349
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        412
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        429
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        464
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        548
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        571
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   623 AA;  69836 MW;  8186D6B5497D1FD4 CRC64;
     MHPGNVWGGS LDAVDSDRIA AEEEERLRNT TEWDRGAIHS QRSELDETQQ GWLLAPQDNW
     RKKKKKYVNL GCVSVSRTVF LWTVGSIAVL FLVVALPIII VKSLPRHKSA PPPPDNYTLA
     LHKALQFFDA QKSGKLPKKN KVSWRGDSGT KDGLPDVVGG LVGGYYDGGS NVKFHFPMAF
     SMTMLSWSLI EYSHKYKAID EYDHMRDVLK WGTDYLLLTF NNSATRLDHI YTQVGGGLRD
     SESPDDIYCW QKPEDMSYDR PVLSSTSAAD LGAEVSAALA AASIVFTDKP DYAKKLKKGA
     ETLYPFFRSK SRRKRYSDGQ PTAQAFYNST SMFDEFMWAG AWLYYATGNK TYIQFATTPS
     VPQTAKAFAN RPELMVPSWN NKLPGAMLLM TRYRLFLNPG FPYENMLNRY HNATGITMCA
     YLKQYNVFNR TSGGLMQLNL GKPRPLEYVA HASFLASLFA DYLNSTGVPG WYCGPTFVEN
     HVLKDFAQSQ IDYILGDNPL KMSYVVGFGK KFPRRVHHRG ATIPNDKKRR SCREGLKYRD
     TKNPNPNNIT GAMVGGPNKF DEFHDLRNNY NASEPTLSGN AGLVAALVSL TSSGGQQIDK
     NTMFNSVPPL YSPTPPPPKA WKP
//