ID   I3ZL31_TERRK            Unreviewed;       395 AA.
AC   I3ZL31;
DT   05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT   05-SEP-2012, sequence version 1.
DT   02-SEP-2026, entry version 68.
DE   RecName: Full=Elongation factor Tu {ECO:0000256|ARBA:ARBA00029554, ECO:0000256|HAMAP-Rule:MF_00118};
DE            Short=EF-Tu {ECO:0000256|HAMAP-Rule:MF_00118};
DE            EC=3.6.5.3 {ECO:0000256|HAMAP-Rule:MF_00118};
GN   Name=tuf {ECO:0000256|HAMAP-Rule:MF_00118};
GN   OrderedLocusNames=Terro_0908 {ECO:0000313|EMBL:AFL87234.1}, Terro_3738
GN   {ECO:0000313|EMBL:AFL89949.1};
OS   Terriglobus roseus (strain DSM 18391 / NRRL B-41598 / KBS 63).
OC   Bacteria; Pseudomonadati; Acidobacteriota; Terriglobia; Terriglobales;
OC   Acidobacteriaceae; Terriglobus.
OX   NCBI_TaxID=926566 {ECO:0000313|EMBL:AFL89949.1, ECO:0000313|Proteomes:UP000006056};
RN   [1] {ECO:0000313|EMBL:AFL89949.1, ECO:0000313|Proteomes:UP000006056}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18391 {ECO:0000313|EMBL:AFL89949.1}, and DSM 18391 / NRRL
RC   B-41598 / KBS 63 {ECO:0000313|Proteomes:UP000006056};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Peters L., Mikhailova N., Munk A.C.C.,
RA   Kyrpides N., Mavromatis K., Ivanova N., Brettin T., Detter J.C., Han C.,
RA   Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P.,
RA   Woyke T., Wu D., Brambilla E., Klenk H.-P., Eisen J.A.;
RT   "Complete genome of Terriglobus roseus DSM 18391.";
RL   Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: GTP hydrolase that promotes the GTP-dependent binding of
CC       aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- FUNCTION: May play an important regulatory role in cell growth and in
CC       the bacterial response to nutrient deprivation.
CC       {ECO:0000256|ARBA:ARBA00058140}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00118};
CC   -!- SUBUNIT: (Microbial infection) Upon infection by bacteriophage Qbeta,
CC       part of the viral RNA-dependent RNA polymerase complex, the other
CC       subunits are the viral replicase catalytic subunit (AC P14647), host
CC       ribosomal protein S1 and EF-Ts. {ECO:0000256|ARBA:ARBA00064283}.
CC   -!- SUBUNIT: Monomer. Heterotetramer composed of two EF-Ts.EF-Tu dimer
CC       complexes. {ECO:0000256|ARBA:ARBA00063778}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC       subfamily. {ECO:0000256|ARBA:ARBA00007249, ECO:0000256|HAMAP-
CC       Rule:MF_00118}.
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DR   EMBL; CP003379; AFL87234.1; -; Genomic_DNA.
DR   EMBL; CP003379; AFL89949.1; -; Genomic_DNA.
DR   RefSeq; WP_014784803.1; NC_018014.1.
DR   AlphaFoldDB; I3ZL31; -.
DR   STRING; 926566.Terro_0908; -.
DR   KEGG; trs:Terro_0908; -.
DR   KEGG; trs:Terro_3738; -.
DR   PATRIC; fig|926566.3.peg.3684; -.
DR   eggNOG; COG0050; Bacteria.
DR   HOGENOM; CLU_007265_0_1_0; -.
DR   OrthoDB; 9804504at2; -.
DR   Proteomes; UP000006056; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd01884; EF_Tu; 1.
DR   CDD; cd03697; EFTU_II; 1.
DR   CDD; cd03707; EFTU_III; 1.
DR   FunFam; 2.40.30.10:FF:000001; Elongation factor Tu; 1.
DR   FunFam; 3.40.50.300:FF:000003; Elongation factor Tu; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   Gene3D; 2.40.30.10; Translation factors; 2.
DR   HAMAP; MF_00118_B; EF_Tu_B; 1.
DR   InterPro; IPR041709; EF-Tu_GTP-bd.
DR   InterPro; IPR050055; EF-Tu_GTPase.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR033720; EFTU_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR   InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR   InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR   NCBIfam; TIGR00485; EF-Tu; 1.
DR   NCBIfam; NF000766; PRK00049.1; 1.
DR   NCBIfam; NF009372; PRK12735.1; 1.
DR   NCBIfam; NF009373; PRK12736.1; 1.
DR   NCBIfam; TIGR00231; small_GTP; 1.
DR   PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF03143; GTP_EFTU_D3; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF50447; Translation proteins; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Elongation factor {ECO:0000256|ARBA:ARBA00022768, ECO:0000256|HAMAP-
KW   Rule:MF_00118};
KW   GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|HAMAP-
KW   Rule:MF_00118};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00118};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW   Rule:MF_00118};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW   Rule:MF_00118}; Reference proteome {ECO:0000313|Proteomes:UP000006056}.
FT   DOMAIN          10..205
FT                   /note="Tr-type G"
FT                   /evidence="ECO:0000259|PROSITE:PS51722"
FT   REGION          19..26
FT                   /note="G1"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01059"
FT   REGION          61..65
FT                   /note="G2"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01059"
FT   REGION          82..85
FT                   /note="G3"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01059"
FT   REGION          137..140
FT                   /note="G4"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01059"
FT   REGION          175..177
FT                   /note="G5"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01059"
FT   BINDING         19..26
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00118"
FT   BINDING         26
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00118"
FT   BINDING         82..86
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00118"
FT   BINDING         137..140
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00118"
SQ   SEQUENCE   395 AA;  43163 MW;  CEDBB9B6792589BB CRC64;
     MGKEKFDRSK PHVNVGTIGH IDHGKTTLTA AITKVLSKHN PKNSFRSFDT IDNAPEERER
     GITIATSHVE YETANRHYAH VDCPGHADYI KNMITGAAQM DGAILVVAAT DGPMPQTKEH
     VLLARQVGVP YIVVFLNKCD AVEDEELIDL VEMEVRELLS KYDFPGDDVP VIRGSALGAL
     NGEAQWEAKI DELMDAVDKN VPQPDRLVDL PFLMPIEDIF SISGRGTVVT GRIERGKIKV
     GEPAQIVGFR DTQNTTVTGV EMFKKQLDEG LAGDNAGLLL RGTAKDDVER GMVLAKPGSI
     TPHTTFKGEI YVLSKEEGGR HTPFFDGYRP QFYFRTTDVT GSAKLPAGTE MVMPGDNIAL
     EITLHTPVAM EKGLRFAIRE GGRTVGAGTI SEIIK
//