ID   F1LSN8_RAT              Unreviewed;       507 AA.
AC   F1LSN8;
DT   03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT   03-MAY-2011, sequence version 1.
DT   02-SEP-2026, entry version 86.
DE   RecName: Full=Natural resistance-associated macrophage protein 1 {ECO:0000256|ARBA:ARBA00015099};
DE   AltName: Full=Solute carrier family 11 member 1 {ECO:0000256|ARBA:ARBA00032337};
GN   Name=Slc11a1 {ECO:0000313|Ensembl:ENSRNOP00000054282.2,
GN   ECO:0000313|RGD:1601463};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116 {ECO:0000313|Ensembl:ENSRNOP00000054282.2, ECO:0000313|Proteomes:UP000002494};
RN   [1] {ECO:0000313|Ensembl:ENSRNOP00000054282.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000054282.2};
RA   Doris P.A., Kalbfleisch T., Li K., Howe K., Wood J.;
RT   "GRCr8: a new rat reference genome assembly contstructed from accurate long
RT   reads and long range scaffolding.";
RL   Submitted (JAN-2024) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSRNOP00000054282.2}
RP   IDENTIFICATION.
RC   STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000054282.2};
RG   Ensembl;
RL   Submitted (JAN-2026) to UniProtKB.
CC   -!- FUNCTION: Macrophage-specific antiporter that fluxes metal ions in
CC       either direction against a proton gradient. Localized to late endosomal
CC       lysosomal membranes, delivers bivalent cations from the cytosol into
CC       these acidic compartments where they may directly affect antimicrobial
CC       activity. Involved in iron metabolism and host natural resistance to
CC       infection with intracellular parasites. Pathogen resistance involves
CC       sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and
CC       eukaryotic catalases and superoxide dismutases, not only to protect the
CC       macrophage against its own generation of reactive oxygen species, but
CC       to deny the cations to the pathogen for synthesis of its protective
CC       enzymes. {ECO:0000256|ARBA:ARBA00035618}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Fe(2+)(in) + H(+)(out) = Fe(2+)(out) + H(+)(in);
CC         Xref=Rhea:RHEA:29439, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000256|ARBA:ARBA00035584};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Mn(2+)(in) + H(+)(out) = Mn(2+)(out) + H(+)(in);
CC         Xref=Rhea:RHEA:73063, ChEBI:CHEBI:15378, ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00048522};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Zn(2+)(in) + H(+)(out) = Zn(2+)(out) + H(+)(in);
CC         Xref=Rhea:RHEA:28839, ChEBI:CHEBI:15378, ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00047695};
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane
CC       {ECO:0000256|ARBA:ARBA00004107}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004107}. Lysosome membrane
CC       {ECO:0000256|ARBA:ARBA00004155}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004155}.
CC   -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family.
CC       {ECO:0000256|ARBA:ARBA00009965}.
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DR   RefSeq; NP_001026828.2; NM_001031658.2.
DR   AlphaFoldDB; F1LSN8; -.
DR   SMR; F1LSN8; -.
DR   Ensembl; ENSRNOT00000057472.5; ENSRNOP00000054282.2; ENSRNOG00000014956.9.
DR   GeneID; 316519; -.
DR   KEGG; rno:316519; -.
DR   CTD; 6556; -.
DR   RGD; 1601463; Slc11a1.
DR   GeneTree; ENSGT00940000160799; -.
DR   Proteomes; UP000002494; Chromosome 9.
DR   Bgee; ENSRNOG00000014956; Expressed in spleen and 18 other cell types or tissues.
DR   ExpressionAtlas; F1LSN8; baseline and differential.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005381; F:iron ion transmembrane transporter activity; IEA:UniProtKB-ARBA.
DR   HAMAP; MF_00221; NRAMP; 1.
DR   InterPro; IPR001046; NRAMP_fam.
DR   NCBIfam; TIGR01197; nramp; 1.
DR   NCBIfam; NF037982; Nramp_1; 1.
DR   PANTHER; PTHR11706:SF52; NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1; 1.
DR   PANTHER; PTHR11706; SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER; 1.
DR   Pfam; PF01566; Nramp; 1.
DR   PRINTS; PR00447; NATRESASSCMP.
PE   1: Evidence at protein level;
KW   Endosome {ECO:0000256|ARBA:ARBA00022753};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron transport {ECO:0000256|ARBA:ARBA00022496};
KW   Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:F1LSN8};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002494};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT   TRANSMEM        48..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        124..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        155..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        197..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        236..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        301..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        354..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        393..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        423..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        451..472
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          1..36
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        8..22
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   507 AA;  55936 MW;  66AA452FBBEA12BE CRC64;
     MIRDKNPQRV NRPSYGSISS LPSPAPQPEP SRNTYLSEKI PIPSTEQLLW VLLWATVLGL
     LCQRLAARLG VVTGKDLGEI CHLYYPKVPR ILLWLTIELA IVGSDMQEVI GTAISFNLLS
     AGRIPLWGGV LITIVDTFFF LFLDNYGLRK LEAFFGFLIT IMALTFGYEY VVARPSQGAL
     LKGLFLPSCP GCGQPELLQA VGIVGAIIMP HNIYLHSALV KSREVDRTRR GDVREANMYF
     LTEATIALFV SFIINLFVMA VFGQAFYQQT NEEAFNICAN SSLHNYAKIF PRDNNTVSVD
     IYQGGVILGC LFGPAALYIW AVGLLAAGQS STMTGTYAGQ FVMEGFLKLR WSRFARVLLT
     RSCAILPTVL VAVFRDLRDL SGLNDLLNVL QSLLLPFAVL PILTFTSMPA VMQEFANGWL
     SKVITSCIMA LVCAINLYFV ISYLPSLPHP AYFGLVALLA IGYLGLTAYL AWTCCIAHGA
     KFLTHSSHQR FLYGLPIEEQ EGREGSG
//