ID   D9IXC6_HYPRU            Unreviewed;       344 AA.
AC   D9IXC6;
DT   05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2010, sequence version 1.
DT   02-SEP-2026, entry version 45.
DE   RecName: Full=AA9 family lytic polysaccharide monooxygenase cel61A {ECO:0000256|ARBA:ARBA00074327};
DE            EC=1.14.99.54 {ECO:0000256|ARBA:ARBA00096574};
DE            EC=1.14.99.56 {ECO:0000256|ARBA:ARBA00047174};
DE   AltName: Full=Cellulase-61A {ECO:0000256|ARBA:ARBA00081970};
DE   AltName: Full=Endo-beta-1,4-glucanase cel61A {ECO:0000256|ARBA:ARBA00078521};
DE   AltName: Full=Glycosyl hydrolase 61 family protein cel61A {ECO:0000256|ARBA:ARBA00080044};
OS   Hypocrea rufa (Trichoderma viride).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=5547 {ECO:0000313|EMBL:ADJ57703.1};
RN   [1] {ECO:0000313|EMBL:ADJ57703.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AS 3.3711 {ECO:0000313|EMBL:ADJ57703.1};
RA   Li X., Miao Y.;
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADJ57703.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=AS 3.3711 {ECO:0000313|EMBL:ADJ57703.1};
RX   PubMed=22057939; DOI=10.1007/s12010-011-9426-5;
RA   Li X.H., Zhang P., Liang S., Zhou F., Wang M.X., Bhaskar R., Malik F.A.,
RA   Niu Y.S., Miao Y.G.;
RT   "Molecular cloning and characterization of a putative cDNA encoding
RT   endoglucanase IV from Trichoderma viride and its expression in Bombyx
RT   mori.";
RL   Appl. Biochem. Biotechnol. 166:309-320(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-
CC         dehydro-beta-D-glucosyl-[(1->4)-beta-D-glucosyl]n-1 + [(1->4)-beta-D-
CC         glucosyl]m + acceptor + H2O.; EC=1.14.99.56;
CC         Evidence={ECO:0000256|ARBA:ARBA00045077};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-
CC         beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-
CC         glucosyl]n + acceptor + H2O.; EC=1.14.99.54;
CC         Evidence={ECO:0000256|ARBA:ARBA00095755};
CC   -!- COFACTOR:
CC       Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC         Evidence={ECO:0000256|ARBA:ARBA00001973};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the polysaccharide monooxygenase AA9 family.
CC       {ECO:0000256|ARBA:ARBA00044502}.
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DR   EMBL; HM222525; ADJ57703.1; -; Genomic_DNA.
DR   AlphaFoldDB; D9IXC6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd21175; LPMO_AA9; 1.
DR   FunFam; 2.70.50.70:FF:000001; Glycoside hydrolase family 61; 1.
DR   Gene3D; 2.70.50.70; -; 1.
DR   InterPro; IPR049892; AA9.
DR   InterPro; IPR005103; AA9_LPMO.
DR   InterPro; IPR000254; CBD.
DR   InterPro; IPR035971; CBD_sf.
DR   PANTHER; PTHR33353:SF36; ENDO-BETA-1,4-GLUCANASE D; 1.
DR   PANTHER; PTHR33353; PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED; 1.
DR   Pfam; PF03443; AA9; 1.
DR   Pfam; PF00734; CBM_1; 1.
DR   SMART; SM00236; fCBD; 1.
DR   SUPFAM; SSF57180; Cellulose-binding domain; 1.
DR   PROSITE; PS00562; CBM1_1; 1.
DR   PROSITE; PS51164; CBM1_2; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW   Copper {ECO:0000256|ARBA:ARBA00023008};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..344
FT                   /note="AA9 family lytic polysaccharide monooxygenase
FT                   cel61A"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003125757"
FT   DOMAIN          307..343
FT                   /note="CBM1"
FT                   /evidence="ECO:0000259|PROSITE:PS51164"
FT   REGION          260..310
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        275..310
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   344 AA;  35566 MW;  F158504FADC9FE17 CRC64;
     MIQKLSNLLV TALAVATGVV GHGHINDIVI NGVWYQAYDP TTFPYESNPP IVVGWTAADL
     DNGFVSPDAY QNPDIICHKN ATNAKGHASV KARDTILFQW VPVPWPHPGP IVDYLANCNG
     DCETVDKTTL EFFKIDGVGL LSGGDPGTWA SDVLISNNNT WVVKIPDNLA PGNYVLRHEI
     IALHSAGQAN GAQNYPQCFN IAVSGSGSLQ PSGVLGTDLY HATDPGVPIN IYTSPLNYII
     PGPTVVSGLP TSVAQGSSAA TATASATAPG GGSGPTSRTT TTARTTQASS RPSSTPPATT
     SAPAGGPTQT LYGQCGGSGY SGPTRCAPPA TCSTLNPYYA QCLN
//