ID   D3UGP7_HELM1            Unreviewed;       742 AA.
AC   D3UGP7;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   02-SEP-2026, entry version 78.
DE   RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR009407};
DE            EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR009407};
DE   AltName: Full=Oxalosuccinate decarboxylase {ECO:0000256|PIRNR:PIRNR009407};
GN   Name=icd {ECO:0000313|EMBL:CBG39668.1};
GN   OrderedLocusNames=HMU04060 {ECO:0000313|EMBL:CBG39668.1};
OS   Helicobacter mustelae (strain ATCC 43772 / CCUG 25715 / CIP 103759 / LMG
OS   18044 / NCTC 12198 / R85-136P) (Campylobacter mustelae).
OC   Bacteria; Pseudomonadati; Campylobacterota; Epsilonproteobacteria;
OC   Campylobacterales; Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=679897 {ECO:0000313|EMBL:CBG39668.1, ECO:0000313|Proteomes:UP000001522};
RN   [1] {ECO:0000313|EMBL:CBG39668.1, ECO:0000313|Proteomes:UP000001522}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43772 / LMG 18044 / NCTC 12198 / 12198
RC   {ECO:0000313|Proteomes:UP000001522};
RX   PubMed=20219135; DOI=10.1186/1471-2164-11-164;
RA   O'Toole P.W., Snelling W.J., Canchaya C., Forde B.M., Hardie K.R.,
RA   Josenhans C., Graham R.L.J., McMullan G., Parkhill J., Belda E.,
RA   Bentley S.D.;
RT   "Comparative genomics and proteomics of Helicobacter mustelae, an
RT   ulcerogenic and carcinogenic gastric pathogen.";
RL   BMC Genomics 11:164-164(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC         Evidence={ECO:0000256|ARBA:ARBA00023554,
CC         ECO:0000256|PIRNR:PIRNR009407};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000256|PIRSR:PIRSR009407-3};
CC   -!- SIMILARITY: Belongs to the monomeric-type IDH family.
CC       {ECO:0000256|ARBA:ARBA00046318, ECO:0000256|PIRNR:PIRNR009407}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FN555004; CBG39668.1; -; Genomic_DNA.
DR   RefSeq; WP_013022759.1; NC_013949.1.
DR   AlphaFoldDB; D3UGP7; -.
DR   STRING; 679897.HMU04060; -.
DR   KEGG; hms:HMU04060; -.
DR   eggNOG; COG2838; Bacteria.
DR   HOGENOM; CLU_025308_1_0_7; -.
DR   Proteomes; UP000001522; Chromosome.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 3.40.718.10; Isopropylmalate Dehydrogenase; 1.
DR   InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR   NCBIfam; TIGR00178; monomer_idh; 1.
DR   PANTHER; PTHR36999:SF1; ISOCITRATE DEHYDROGENASE (NADP(+)); 1.
DR   PANTHER; PTHR36999; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR   Pfam; PF03971; IDH; 1.
DR   PIRSF; PIRSF009407; IDH_monmr; 1.
DR   SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
PE   3: Inferred from homology;
KW   Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW   ECO:0000256|PIRNR:PIRNR009407};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR009407-3};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR009407-3};
KW   NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|PIRNR:PIRNR009407};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|PIRNR:PIRNR009407};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001522};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW   ECO:0000256|PIRNR:PIRNR009407}.
FT   BINDING         129..136
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT   BINDING         142
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT   BINDING         345
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT   BINDING         547
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT   BINDING         548
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT   BINDING         552
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT   SITE            252
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT   SITE            415
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
SQ   SEQUENCE   742 AA;  82856 MW;  DAF8E1C16F4A3276 CRC64;
     MKITYTLTDE SPALATYSFL PIVRAFLHTA GIEVESADIS LAGRILAQFP QHLTPSQRIP
     NALEALGELV KHKDANLIKT PNISASIPQL KNAIAELQAK GYNVPNYPDN PKNQEEEEIK
     ENYQKILGSA VNPVLRQGNS DRRSTKAVKQ YAKKNPYKIA PFDSNSKTIV SYMQEGDFFS
     NEKAVWIKEP TEARIVFENK EGKREILKKG LKLEKEEILD ATFMDVEALD RFYVDQIALC
     KKQDILFSLH LKATMMKVSD PVIFGHAVRT FFAELFEEFA DELHALGVNP NNGISELLQK
     IQTSAKKDAI LQKYEEILHK SAKISMVNSD KGITNLHVPS DVIVDASMPA MLKNGAKLWD
     KEGRECDTNA VIPDRTYATI YEAVIEDLHK FGPLDPSKLG SVANVGLMAK KAQEYGSHDK
     TFIAKEAGSF KILDHQDRVL LEHAVQKGDI YRANQAKHDA IINWIDLGIQ RSETSHTPAI
     FWLDEKRPSN KIMIEMVQKR LQERGIALKS SHGANLEILP PKAACLKSLE IIRNGGDVIS
     ITGNVLRDYL TDLFPILELG TSAKMLSIVP MLSGGAMFET GAGGSAPKQV EQLLLENHLR
     WDSLGEFLAL QASLEFFAQQ NHNARAKVLA DALDVAIGKW LDNNKAPSRN AGQDDNRTSH
     FYLAMYFAEA LAKQKEDTAL QERFAPIASE LQAKEQKIKE EFLSKEGVSV DLGGYYKFDD
     EKAIKIMRPS ATFNEILHKI AQ
//