ID   D2TTS0_CITRI            Unreviewed;       317 AA.
AC   D2TTS0;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   02-SEP-2026, entry version 81.
DE   RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE            EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN   Name=rluC {ECO:0000313|EMBL:CBG87914.1};
GN   OrderedLocusNames=ROD_11481 {ECO:0000313|EMBL:CBG87914.1};
OS   Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=637910 {ECO:0000313|EMBL:CBG87914.1, ECO:0000313|Proteomes:UP000001889};
RN   [1] {ECO:0000313|EMBL:CBG87914.1, ECO:0000313|Proteomes:UP000001889}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ICC168 {ECO:0000313|EMBL:CBG87914.1,
RC   ECO:0000313|Proteomes:UP000001889};
RX   PubMed=19897651; DOI=10.1128/JB.01144-09;
RA   Petty N.K., Bulgin R., Crepin V.F., Cerdeno-Tarraga A.M., Schroeder G.N.,
RA   Quail M.A., Lennard N., Corton C., Barron A., Clark L., Toribio A.L.,
RA   Parkhill J., Dougan G., Frankel G., Thomson N.R.;
RT   "The Citrobacter rodentium genome sequence reveals convergent evolution
RT   with human pathogenic Escherichia coli.";
RL   J. Bacteriol. 192:525-538(2010).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 955, 2504 and 2580 in 23S ribosomal RNA.
CC       {ECO:0000256|ARBA:ARBA00002876}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000256|RuleBase:RU362028};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(955/2504/2580) in 23S rRNA =
CC         pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC         Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.24;
CC         Evidence={ECO:0000256|ARBA:ARBA00000381};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR   EMBL; FN543502; CBG87914.1; -; Genomic_DNA.
DR   RefSeq; WP_012905435.1; NC_013716.1.
DR   AlphaFoldDB; D2TTS0; -.
DR   STRING; 637910.ROD_11481; -.
DR   PaxDb; 637910-ROD_11481; -.
DR   KEGG; cro:ROD_11481; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_1_1_6; -.
DR   OrthoDB; 9807829at2; -.
DR   Proteomes; UP000001889; Chromosome.
DR   GO; GO:0160141; F:23S rRNA pseudouridine(955/2504/2580) synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:TreeGrafter.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   FunFam; 3.10.290.10:FF:000010; Pseudouridine synthase; 1.
DR   FunFam; 3.30.2350.10:FF:000007; Pseudouridine synthase; 1.
DR   Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR050188; RluA_PseudoU_synthase.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   NCBIfam; NF008249; PRK11025.1; 1.
DR   NCBIfam; TIGR00005; rluA_subfam; 1.
DR   PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR   PANTHER; PTHR21600:SF92; RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE C; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU362028};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001889};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU00182}; rRNA processing {ECO:0000256|ARBA:ARBA00022552}.
FT   DOMAIN          20..79
FT                   /note="RNA-binding S4"
FT                   /evidence="ECO:0000259|SMART:SM00363"
FT   ACT_SITE        144
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ   SEQUENCE   317 AA;  35670 MW;  5D6A163A4B9B0194 CRC64;
     MKTETPSVKI VAITADEAGQ RIDNFLRTQL KGVPKSMIYR ILRKGEVRVN KKRIKPEYKL
     EAGDEIRIPP VRVAEREEEA VSPHLQKVAA LADVILYEDD HILVLNKPSG TAVHGGSGLS
     FGVIEGLRAL RPEARFLELV HRLDRDTSGV LLVAKKRSAL RSLHEQLREK GMQKDYLALV
     RGQWQSHVKN VQAPLLKNIL QSGERIVRVS QEGKPSETRF KVEERYAFAT LVRCSPVTGR
     THQIRVHTQY AGHPIAFDDR YGDREFDKQL AATGLSRLFL HAAALKFTHP GTGEVLRIEA
     PMDGQLKHCL QVLRKPG
//