ID   D2QSS8_SPILD            Unreviewed;       379 AA.
AC   D2QSS8;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   02-SEP-2026, entry version 76.
DE   RecName: Full=2-isopropylmalate synthase {ECO:0000256|ARBA:ARBA00012973};
DE            EC=2.3.3.13 {ECO:0000256|ARBA:ARBA00012973};
GN   OrderedLocusNames=Slin_5897 {ECO:0000313|EMBL:ADB41860.1};
OS   Spirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896 / Claus 1).
OC   Bacteria; Pseudomonadati; Bacteroidota; Cytophagia; Cytophagales;
OC   Cytophagaceae; Spirosoma.
OX   NCBI_TaxID=504472 {ECO:0000313|EMBL:ADB41860.1, ECO:0000313|Proteomes:UP000002028};
RN   [1] {ECO:0000313|EMBL:ADB41860.1, ECO:0000313|Proteomes:UP000002028}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33905 / DSM 74 / LMG 10896 / Claus 1
RC   {ECO:0000313|Proteomes:UP000002028};
RX   PubMed=21304700;
RA   Lail K., Sikorski J., Saunders E., Lapidus A., Glavina Del Rio T.,
RA   Copeland A., Tice H., Cheng J.-F., Lucas S., Nolan M., Bruce D.,
RA   Goodwin L., Pitluck S., Ivanova N., Mavromatis K., Ovchinnikova G.,
RA   Pati A., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.-J.,
RA   Jeffries C.D., Chain P., Brettin T., Detter J.C., Schuetze A., Rohde M.,
RA   Tindall B.J., Goeker M., Bristow J., Eisen J.A., Markowitz V.,
RA   Hugenholtz P., Kyrpides N.C., Klenk H.-P., Chen F.;
RT   "Complete genome sequence of Spirosoma linguale type strain (1).";
RL   Stand. Genomic Sci. 2:176-185(2010).
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 1/4.
CC       {ECO:0000256|ARBA:ARBA00004689}.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. LeuA type 1 subfamily. {ECO:0000256|ARBA:ARBA00009396}.
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DR   EMBL; CP001769; ADB41860.1; -; Genomic_DNA.
DR   RefSeq; WP_012930350.1; NC_013730.1.
DR   AlphaFoldDB; D2QSS8; -.
DR   STRING; 504472.Slin_5897; -.
DR   KEGG; sli:Slin_5897; -.
DR   eggNOG; COG0119; Bacteria.
DR   HOGENOM; CLU_022158_3_1_10; -.
DR   Proteomes; UP000002028; Chromosome.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:L-leucine biosynthetic process; IEA:TreeGrafter.
DR   CDD; cd07940; DRE_TIM_IPMS; 1.
DR   FunFam; 1.10.238.260:FF:000001; 2-isopropylmalate synthase; 1.
DR   FunFam; 3.20.20.70:FF:000010; 2-isopropylmalate synthase; 1.
DR   Gene3D; 1.10.238.260; -; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR050073; 2-IPM_HCS-like.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR054691; LeuA/HCS_post-cat.
DR   InterPro; IPR000891; PYR_CT.
DR   NCBIfam; NF002086; PRK00915.1-3; 1.
DR   PANTHER; PTHR10277:SF9; 2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED; 1.
DR   PANTHER; PTHR10277; HOMOCITRATE SYNTHASE-RELATED; 1.
DR   Pfam; PF22617; HCS_D2; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW   Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW   Leucine biosynthesis {ECO:0000256|ARBA:ARBA00022430};
KW   Manganese {ECO:0000256|ARBA:ARBA00023211};
KW   Pyruvate {ECO:0000313|EMBL:ADB41860.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002028};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003523}.
FT   DOMAIN          5..266
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000259|PROSITE:PS50991"
SQ   SEQUENCE   379 AA;  41683 MW;  563234C1818D2CFE CRC64;
     MSQRVYIFDT TLRDGEQVPG CQLTTEEKIV VAKELERMGV DVIEAGFPIS SPGDFRSVVE
     ISKAVTEPTI CALSRAVKGD IDAAGEALKW AKRGRIHTGI GSSDIHIRNK FNSTRENILE
     QAIAAVKHAK SFVEDVEFYA EDAGRADLAF LAQLTEAVIK AGATVVNIPD TTGYCLPDEY
     GKKIAYIYEH VSNVHQATIS IHCHNDLGLA TANTLAGVMN GARQVEVTMN GIGERAGNTS
     LEEVVMALKV KKELGLHTNI DTTRLFPVSN LVSQMMRMPV QANKAIVGRN AFAHSSGIHQ
     DGFLKHSENY EIMNPHDVGV PKSSIVLTAR SGRHALKHRL ELLGFRYDKP TLDGIYTRFL
     DMADVRKEVN DKDLMELVR
//