ID   C3YFB2_BRAFL            Unreviewed;       116 AA.
AC   C3YFB2;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   02-SEP-2026, entry version 78.
DE   RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 {ECO:0000256|ARBA:ARBA00018947, ECO:0000256|RuleBase:RU361136};
DE            Short=Oligosaccharyl transferase subunit DAD1 {ECO:0000256|RuleBase:RU361136};
GN   Name=LOC118418410 {ECO:0000313|RefSeq:XP_035680197.1};
OS   Branchiostoma floridae (Florida lancelet) (Amphioxus).
OC   Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC   Branchiostomatidae; Branchiostoma.
OX   NCBI_TaxID=7739 {ECO:0000313|Proteomes:UP000001554, ECO:0000313|RefSeq:XP_035680197.1};
RN   [1] {ECO:0000313|Proteomes:UP000001554}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S238N-H82 {ECO:0000313|Proteomes:UP000001554};
RX   PubMed=32313176;
RA   Simakov O., Marletaz F., Yue J.X., O'Connell B., Jenkins J., Brandt A.,
RA   Calef R., Tung C.H., Huang T.K., Schmutz J., Satoh N., Yu J.K.,
RA   Putnam N.H., Green R.E., Rokhsar D.S.;
RT   "Deeply conserved synteny resolves early events in vertebrate evolution.";
RL   Nat. Ecol. Evol. 0:0-0(2020).
RN   [2] {ECO:0000313|RefSeq:XP_035680197.1}
RP   IDENTIFICATION.
RC   STRAIN=S238N-H82 {ECO:0000313|RefSeq:XP_035680197.1};
RC   TISSUE=Testes {ECO:0000313|RefSeq:XP_035680197.1};
RG   RefSeq;
RL   Submitted (JAN-2026) to UniProtKB.
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC       the complex associates with the Sec61 complex at the channel-forming
CC       translocon complex that mediates protein translocation across the
CC       endoplasmic reticulum (ER). All subunits are required for a maximal
CC       enzyme activity. {ECO:0000256|RuleBase:RU361136}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|ARBA:ARBA00004922, ECO:0000256|RuleBase:RU361136}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000256|RuleBase:RU361136}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|ARBA:ARBA00004477, ECO:0000256|RuleBase:RU361136}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004477,
CC       ECO:0000256|RuleBase:RU361136}.
CC   -!- SIMILARITY: Belongs to the DAD/OST2 family.
CC       {ECO:0000256|ARBA:ARBA00009386, ECO:0000256|RuleBase:RU361136}.
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DR   RefSeq; XP_035680197.1; XM_035824304.1.
DR   AlphaFoldDB; C3YFB2; -.
DR   STRING; 7739.C3YFB2; -.
DR   GeneID; 118418410; -.
DR   KEGG; bfo:118418410; -.
DR   eggNOG; KOG1746; Eukaryota.
DR   OMA; HIILHIV; -.
DR   OrthoDB; 445566at2759; -.
DR   Proteomes; UP000001554; Chromosome 6.
DR   GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR   InterPro; IPR003038; DAD/Ost2.
DR   PANTHER; PTHR10705; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1; 1.
DR   PANTHER; PTHR10705:SF0; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1; 1.
DR   Pfam; PF02109; DAD; 1.
DR   PIRSF; PIRSF005588; DAD; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824,
KW   ECO:0000256|RuleBase:RU361136};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU361136};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001554};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU361136};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU361136}.
FT   TRANSMEM        33..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361136"
FT   TRANSMEM        58..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361136"
FT   TRANSMEM        96..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361136"
SQ   SEQUENCE   116 AA;  13116 MW;  F832CE79F874A0B7 CRC64;
     MATSQANVFT VVSKFYDEYV TTTPQRLKII DAYLAYIMLT GIFQFGYCLL VGTFPFNSFL
     SGFISCVASF VLGVCLRVQV NPQNRKDFLH ISPERAFADF VFAHIILHLV VMNFIG
//