ID   B9GN77_POPTR            Unreviewed;       683 AA.
AC   B9GN77;
DT   24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   24-MAR-2009, sequence version 1.
DT   04-NOV-2026, entry version 87.
DE   RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE            EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN   ORFNames=POPTR_002G110100 {ECO:0000313|EMBL:PNT49104.1};
OS   Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp.
OS   trichocarpa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=3694 {ECO:0000313|EMBL:PNT49104.1, ECO:0000313|Proteomes:UP000006729};
RN   [1] {ECO:0000313|EMBL:PNT49104.1, ECO:0000313|Proteomes:UP000006729}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nisqually {ECO:0000313|Proteomes:UP000006729};
RX   PubMed=16973872; DOI=10.1126/science.1128691;
RA   Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I.,
RA   Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J.,
RA   Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W.,
RA   Brun A., Brunner A., Busov V., Campbell M., Carlson J., Chalot M.,
RA   Chapman J., Chen G.L., Cooper D., Coutinho P.M., Couturier J., Covert S.,
RA   Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A.,
RA   Depamphilis C., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J.,
RA   Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A.,
RA   Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B.,
RA   Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S.,
RA   Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjarvi J., Karlsson J.,
RA   Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F.,
RA   Leebens-Mack J., Leple J.C., Locascio P., Lou Y., Lucas S., Martin F.,
RA   Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O.,
RA   Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J.,
RA   Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J.,
RA   Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A.,
RA   Tsai C.J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S.,
RA   Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y.,
RA   Rokhsar D.;
RT   "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).";
RL   Science 313:1596-1604(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC         (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC         rhamnogalacturonan I domains in ramified hairy regions of pectin
CC         leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC         unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC         EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC       {ECO:0000256|ARBA:ARBA00010418}.
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DR   EMBL; CM009291; PNT49104.1; -; Genomic_DNA.
DR   AlphaFoldDB; B9GN77; -.
DR   STRING; 3694.B9GN77; -.
DR   Gramene; Potri.002G110100.1.v4.1; Potri.002G110100.1.v4.1; Potri.002G110100.v4.1.
DR   Gramene; Potri.002G110100.4.v4.1; Potri.002G110100.4.v4.1; Potri.002G110100.v4.1.
DR   eggNOG; ENOG502QQM5; Eukaryota.
DR   HOGENOM; CLU_015099_1_0_1; -.
DR   InParanoid; B9GN77; -.
DR   OMA; ACTCMMM; -.
DR   OrthoDB; 2130367at2759; -.
DR   Proteomes; UP000006729; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd10317; RGL4_C; 1.
DR   CDD; cd10316; RGL4_M; 1.
DR   CDD; cd10320; RGL4_N; 1.
DR   FunFam; 2.60.40.1120:FF:000033; Rhamnogalacturonate lyase B; 1.
DR   Gene3D; 2.70.98.10; -; 1.
DR   Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   InterPro; IPR013784; Carb-bd-like_fold.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR029413; RG-lyase_II.
DR   InterPro; IPR029411; RG-lyase_III.
DR   InterPro; IPR010325; Rhamnogal_lyase.
DR   PANTHER; PTHR32018:SF40; RHAMNOGALACTURONAN ENDOLYASE; 1.
DR   PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR   Pfam; PF14683; CBM-like; 1.
DR   Pfam; PF14686; fn3_3; 1.
DR   Pfam; PF06045; Rhamnogal_lyase; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE   3: Inferred from homology;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006729};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..683
FT                   /note="rhamnogalacturonan endolyase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5030165843"
FT   DOMAIN          401..473
FT                   /note="Rhamnogalacturonan lyase"
FT                   /evidence="ECO:0000259|Pfam:PF14686"
FT   DOMAIN          487..673
FT                   /note="Rhamnogalacturonan lyase"
FT                   /evidence="ECO:0000259|Pfam:PF14683"
SQ   SEQUENCE   683 AA;  76817 MW;  D8F1936A6259C8CA CRC64;
     MGSGKILCTI LSLFSQFMAA FSNLQQNENP SMGFHGGEKG TGSSLLSEVV QLHIQKDYVV
     LDNGILQVTL SNPGGHVTGI QYNGLENLLE TDNGESDRGY WDVVWSGEGV TRKKGNLDRL
     DGTNLTVVVE SEEKVEISFT RMWNSSLQAK VVPLNFDKRY VMLRGSSGFY TYAIYEHLRG
     WPAFDLDNTR IVFKLTRQKF RYMAIADNRQ RYMPLPEDRS PERGQTLAYP EAVLLVHPVE
     PEFEGEVDDK YAYSCESKDI SVHGWISADP LVGFWQITPS HEFRTGGPLK QFLTSHVGPT
     NLGVMHSTHY AGADVTIKIG PNEPWKKVYG PVFAYVNSLS DGRDPLSLWK DAKKQMMNEV
     HKWPYDFIAS EDFPPSKQRG SVGGRLLVLE RYVSNATISA EGAYVGLAAP GEAGSWQLES
     KGYQFWTKTD EGGNFTINGI RPGDYNLYAW VPGFIGDYKF TSVININSGS NIDIGDLVYE
     APRNGSTLWE IGFPDRSAAE FYIPDANPKY INKLYLKQER YRQYGLWERY AELYPKADLV
     FTVGKSNHTT DWFFAQVTRK KDNTTYEGTT WQIKFLLDEV DERAAYKLRM ALATANVAEL
     EVRVNEPNAN PVFSTGEIGK DNTIARHGIH GLYRLFNVDI PGAQLLIGNN TIFLTQTASI
     SPFQGVMYDY IRLEGPPPPH KAL
//