ID   B8GFM2_METPE            Unreviewed;       325 AA.
AC   B8GFM2;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   02-SEP-2026, entry version 69.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   OrderedLocusNames=Mpal_0703 {ECO:0000313|EMBL:ACL16070.1};
OS   Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC   Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC   Methanomicrobia; Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX   NCBI_TaxID=521011 {ECO:0000313|EMBL:ACL16070.1, ECO:0000313|Proteomes:UP000002457};
RN   [1] {ECO:0000313|EMBL:ACL16070.1, ECO:0000313|Proteomes:UP000002457}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c
RC   {ECO:0000313|Proteomes:UP000002457};
RX   PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA   Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA   Yavitt J.B., Zinder S.H.;
RT   "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT   Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL   Genome Announc. 3:e01280-15(2015).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine +
CC         H(+); Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00047942};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       {ECO:0000256|ARBA:ARBA00006594}.
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DR   EMBL; CP001338; ACL16070.1; -; Genomic_DNA.
DR   RefSeq; WP_012617389.1; NC_011832.1.
DR   AlphaFoldDB; B8GFM2; -.
DR   STRING; 521011.Mpal_0703; -.
DR   REBASE; 19798; M.MpaEORF703P.
DR   GeneID; 7271849; -.
DR   KEGG; mpl:Mpal_0703; -.
DR   eggNOG; arCOG03416; Archaea.
DR   HOGENOM; CLU_063430_0_0_2; -.
DR   OrthoDB; 372040at2157; -.
DR   Proteomes; UP000002457; Chromosome.
DR   GO; GO:1904047; F:S-adenosyl-L-methionine binding; IEA:TreeGrafter.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:TreeGrafter.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:TreeGrafter.
DR   Gene3D; 1.10.1020.10; Adenine-specific Methyltransferase, Domain 2; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR023095; Ade_MeTrfase_dom_2.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR012263; M_m6A_EcoRV.
DR   InterPro; IPR012327; MeTrfase_D12.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; TIGR00571; dam; 1.
DR   PANTHER; PTHR30481; DNA ADENINE METHYLASE; 1.
DR   PANTHER; PTHR30481:SF3; DNA ADENINE METHYLASE; 1.
DR   Pfam; PF02086; MethyltransfD12; 1.
DR   PIRSF; PIRSF000398; M_m6A_EcoRV; 1.
DR   PRINTS; PR00505; D12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:ACL16070.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002457};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ACL16070.1}.
SQ   SEQUENCE   325 AA;  36957 MW;  F7D324E00D0DA6E6 CRC64;
     MRPSKPGTCI IRPAVKPTAK PMARPFLKWA GGKTQLLDQL QRRLPGEIQQ GEITHYLEPF
     VGGGAVFFAI NQCYSFECSD ICDINEELVL AYTVVKRDVE ALIEVLDRME TEYYALDEAG
     RSGYFYQVRE IFNAEKEGTC FDRYTRSWVD RAALLIFLNH TCFNGLYRVN AKGHFNVPFG
     RYRSPRILDA ENLRLVSKVL ERTAIHHGDF TGCEGMVNER TFVYLDPPYR PLNSTAQFTG
     YYRAGFNEGD QVRLAEFIGT LDRRGAKIML SNSDPCNEDP DDTFFDTLYV GYTIDRVSAK
     RTINSNAGRR GAISELVITN YPPVI
//