ID   B8GDG4_METPE            Unreviewed;       384 AA.
AC   B8GDG4;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   28-JAN-2026, entry version 74.
DE   RecName: Full=Pyruvate synthase subunit PorA {ECO:0000256|ARBA:ARBA00044787};
DE            EC=1.2.7.1 {ECO:0000256|ARBA:ARBA00012822};
DE   AltName: Full=Pyruvate oxidoreductase alpha chain {ECO:0000256|ARBA:ARBA00044814};
DE   AltName: Full=Pyruvic-ferredoxin oxidoreductase subunit alpha {ECO:0000256|ARBA:ARBA00044811};
GN   OrderedLocusNames=Mpal_2014 {ECO:0000313|EMBL:ACL17315.1};
OS   Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC   Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC   Methanomicrobia; Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX   NCBI_TaxID=521011 {ECO:0000313|EMBL:ACL17315.1, ECO:0000313|Proteomes:UP000002457};
RN   [1] {ECO:0000313|EMBL:ACL17315.1, ECO:0000313|Proteomes:UP000002457}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c
RC   {ECO:0000313|Proteomes:UP000002457};
RX   PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA   Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA   Yavitt J.B., Zinder S.H.;
RT   "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT   Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL   Genome Announc. 3:e01280-15(2015).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced
CC         [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+);
CC         Xref=Rhea:RHEA:12765, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC         ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=1.2.7.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00049357};
CC   -!- SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma
CC       chain. {ECO:0000256|ARBA:ARBA00011595}.
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DR   EMBL; CP001338; ACL17315.1; -; Genomic_DNA.
DR   RefSeq; WP_012618634.1; NC_011832.1.
DR   AlphaFoldDB; B8GDG4; -.
DR   STRING; 521011.Mpal_2014; -.
DR   GeneID; 7271995; -.
DR   KEGG; mpl:Mpal_2014; -.
DR   eggNOG; arCOG01608; Archaea.
DR   HOGENOM; CLU_002569_5_0_2; -.
DR   OrthoDB; 372068at2157; -.
DR   Proteomes; UP000002457; Chromosome.
DR   GO; GO:0019164; F:pyruvate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006979; P:response to oxidative stress; IEA:TreeGrafter.
DR   CDD; cd07034; TPP_PYR_PFOR_IOR-alpha_like; 1.
DR   FunFam; 3.40.50.920:FF:000010; Pyruvate ferredoxin oxidoreductase, alpha subunit; 1.
DR   FunFam; 3.40.50.970:FF:000012; Pyruvate:ferredoxin (Flavodoxin) oxidoreductase; 1.
DR   Gene3D; 3.40.50.920; -; 1.
DR   Gene3D; 3.40.50.970; -; 1.
DR   InterPro; IPR033412; PFOR_II.
DR   InterPro; IPR050722; Pyruvate:ferred/Flavod_OxRd.
DR   InterPro; IPR053390; Pyruvate_synthase_PorA.
DR   InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR009014; Transketo_C/PFOR_II.
DR   NCBIfam; NF040682; PorA_Arch; 1.
DR   PANTHER; PTHR32154; PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED; 1.
DR   PANTHER; PTHR32154:SF0; PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED; 1.
DR   Pfam; PF17147; PFOR_II; 1.
DR   Pfam; PF01855; POR_N; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 1.
DR   SUPFAM; SSF52922; TK C-terminal domain-like; 1.
PE   4: Predicted;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Pyruvate {ECO:0000313|EMBL:ACL17315.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002457}.
FT   DOMAIN          13..236
FT                   /note="Pyruvate flavodoxin/ferredoxin oxidoreductase
FT                   pyrimidine binding"
FT                   /evidence="ECO:0000259|Pfam:PF01855"
FT   DOMAIN          260..361
FT                   /note="Pyruvate:ferredoxin oxidoreductase core"
FT                   /evidence="ECO:0000259|Pfam:PF17147"
SQ   SEQUENCE   384 AA;  42169 MW;  FBDADC44C71363CD CRC64;
     MLKILEGSIA VAEAVKQCRP QVIAAYPITP QTHIVEALAD IVANGELDCE YITVESEFSA
     LSACLGASAA GSRTYSATTS QGLALMFEVC FNVAGLRLPI VMTIANRALG APLSIWNDQQ
     DSISLRDSGW LQFYAEDNQE ATDLHYIAYR VAEHHDVLLP AFVCFDGFIL SHTYEPVDML
     SQEDADRFMK PFDPLYRLDA AAPVSMGMYA TPEYYLEFRY EHDQAQHRAK KIIAEVGREF
     GELFGRDYSA LVEGYRLEDA EVALVAMGSL CGTTKDAIDE MRAAGKKVGL LKIRTFRPFP
     TEEVAAALKH VQTVAVLDKN ISLGAKGAVA LEVKDALYNS GVRVFDYILG LGGRDVRKRD
     IAAIVELAES GKGDLFYGLR TEVL
//