ID GAL1_ECO55 Reviewed; 382 AA. AC B7LAF8; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 24-MAR-2009, sequence version 1. DT 02-SEP-2026, entry version 87. DE RecName: Full=Galactokinase {ECO:0000255|HAMAP-Rule:MF_00246}; DE EC=2.7.1.6 {ECO:0000255|HAMAP-Rule:MF_00246}; DE AltName: Full=Galactose kinase {ECO:0000255|HAMAP-Rule:MF_00246}; GN Name=galK {ECO:0000255|HAMAP-Rule:MF_00246}; GN OrderedLocusNames=EC55989_0736; OS Escherichia coli (strain 55989 / EAEC). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; OC Enterobacterales; Enterobacteriaceae; Escherichia. OX NCBI_TaxID=585055; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=55989 / EAEC; RX PubMed=19165319; DOI=10.1371/journal.pgen.1000344; RA Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., RA Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., RA Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E., RA Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., RA Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., RA Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., RA Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.; RT "Organised genome dynamics in the Escherichia coli species results in RT highly diverse adaptive paths."; RL PLoS Genet. 5:E1000344-E1000344(2009). CC -!- FUNCTION: Catalyzes the transfer of the gamma-phosphate of ATP to D- CC galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). CC {ECO:0000255|HAMAP-Rule:MF_00246}. CC -!- CATALYTIC ACTIVITY: CC Reaction=alpha-D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP CC + H(+); Xref=Rhea:RHEA:13553, ChEBI:CHEBI:15378, ChEBI:CHEBI:28061, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58336, ChEBI:CHEBI:456216; EC=2.7.1.6; CC Evidence={ECO:0000255|HAMAP-Rule:MF_00246}; CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism. CC {ECO:0000255|HAMAP-Rule:MF_00246}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00246}. CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily. CC {ECO:0000255|HAMAP-Rule:MF_00246}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CU928145; CAU96604.1; -; Genomic_DNA. DR RefSeq; WP_000053415.1; NC_011748.1. DR AlphaFoldDB; B7LAF8; -. DR SMR; B7LAF8; -. DR GeneID; 75170756; -. DR KEGG; eck:EC55989_0736; -. DR HOGENOM; CLU_017814_2_1_6; -. DR UniPathway; UPA00214; -. DR Proteomes; UP000000746; Chromosome. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0004335; F:galactokinase activity; IEA:UniProtKB-UniRule. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0046835; P:carbohydrate phosphorylation; IEA:InterPro. DR GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniRule. DR FunFam; 3.30.230.10:FF:000017; Galactokinase; 1. DR FunFam; 3.30.70.890:FF:000001; Galactokinase; 1. DR Gene3D; 3.30.230.10; -; 1. DR Gene3D; 3.30.70.890; GHMP kinase, C-terminal domain; 1. DR HAMAP; MF_00246; Galactokinase; 1. DR InterPro; IPR000705; Galactokinase. DR InterPro; IPR022963; Galactokinase_bac. DR InterPro; IPR019741; Galactokinase_CS. DR InterPro; IPR019539; GalKase_N. DR InterPro; IPR013750; GHMP_kinase_C_dom. DR InterPro; IPR036554; GHMP_kinase_C_sf. DR InterPro; IPR006204; GHMP_kinase_N_dom. DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS. DR InterPro; IPR006206; Mevalonate/galactokinase. DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF. DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr. DR NCBIfam; TIGR00131; gal_kin; 1. DR NCBIfam; NF003472; PRK05101.1; 1. DR PANTHER; PTHR10457:SF7; GALACTOKINASE-RELATED; 1. DR PANTHER; PTHR10457; MEVALONATE KINASE/GALACTOKINASE; 1. DR Pfam; PF10509; GalKase_gal_bdg; 1. DR Pfam; PF08544; GHMP_kinases_C; 1. DR Pfam; PF00288; GHMP_kinases_N; 1. DR PIRSF; PIRSF000530; Galactokinase; 1. DR PRINTS; PR00473; GALCTOKINASE. DR PRINTS; PR00959; MEVGALKINASE. DR SUPFAM; SSF55060; GHMP Kinase, C-terminal domain; 1. DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1. DR PROSITE; PS00106; GALACTOKINASE; 1. DR PROSITE; PS00627; GHMP_KINASES_ATP; 1. PE 3: Inferred from homology; KW ATP-binding; Carbohydrate metabolism; Cytoplasm; Galactose metabolism; KW Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; KW Transferase. FT CHAIN 1..382 FT /note="Galactokinase" FT /id="PRO_1000125378" FT ACT_SITE 174 FT /note="Proton acceptor" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" FT BINDING 34..37 FT /ligand="substrate" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" FT BINDING 124..130 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" FT BINDING 130 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" FT BINDING 162 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" FT BINDING 223 FT /ligand="substrate" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" FT SITE 28 FT /note="Transition state stabilizer" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00246" SQ SEQUENCE 382 AA; 41442 MW; 7D886CE6FC48F0A1 CRC64; MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL GKKDHALLID CRSLGTKAVS MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP AVQQAVAEQY EAKTGIKETF YVCKPSQGAG QC //