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<entry dataset="Swiss-Prot" created="2009-04-14" modified="2026-06-10" version="81" xmlns="http://uniprot.org/uniprot">
  <accession>B6I6T9</accession>
  <name>GLK_ECOSE</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">Glucokinase</fullName>
      <ecNumber evidence="1">2.7.1.2</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">Glucose kinase</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">glk</name>
    <name type="ordered locus">ECSE_2685</name>
  </gene>
  <organism>
    <name type="scientific">Escherichia coli (strain SE11)</name>
    <dbReference type="NCBI Taxonomy" id="409438"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Enterobacterales</taxon>
      <taxon>Enterobacteriaceae</taxon>
      <taxon>Escherichia</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2008" name="DNA Res." volume="15" first="375" last="386">
      <title>Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult.</title>
      <authorList>
        <person name="Oshima K."/>
        <person name="Toh H."/>
        <person name="Ogura Y."/>
        <person name="Sasamoto H."/>
        <person name="Morita H."/>
        <person name="Park S.-H."/>
        <person name="Ooka T."/>
        <person name="Iyoda S."/>
        <person name="Taylor T.D."/>
        <person name="Hayashi T."/>
        <person name="Itoh K."/>
        <person name="Hattori M."/>
      </authorList>
      <dbReference type="PubMed" id="18931093"/>
      <dbReference type="DOI" id="10.1093/dnares/dsn026"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>SE11</strain>
    </source>
  </reference>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
      <dbReference type="Rhea" id="RHEA:17825"/>
      <dbReference type="ChEBI" id="CHEBI:4167"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
      <dbReference type="ChEBI" id="CHEBI:61548"/>
      <dbReference type="ChEBI" id="CHEBI:456216"/>
      <dbReference type="EC" id="2.7.1.2"/>
    </reaction>
  </comment>
  <comment type="subcellular location">
    <subcellularLocation>
      <location evidence="1">Cytoplasm</location>
    </subcellularLocation>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the bacterial glucokinase family.</text>
  </comment>
  <dbReference type="EC" id="2.7.1.2" evidence="1"/>
  <dbReference type="EMBL" id="AP009240">
    <property type="protein sequence ID" value="BAG78209.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_000170346.1">
    <property type="nucleotide sequence ID" value="NC_011415.1"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="B6I6T9"/>
  <dbReference type="SMR" id="B6I6T9"/>
  <dbReference type="GeneID" id="75172507"/>
  <dbReference type="GeneID" id="75202543"/>
  <dbReference type="KEGG" id="ecy:ECSE_2685"/>
  <dbReference type="HOGENOM" id="CLU_042582_1_0_6"/>
  <dbReference type="GO" id="GO:0005829">
    <property type="term" value="C:cytosol"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005524">
    <property type="term" value="F:ATP binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005536">
    <property type="term" value="F:D-glucose binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="GO" id="GO:0004340">
    <property type="term" value="F:glucokinase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0006096">
    <property type="term" value="P:glycolytic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="CDD" id="cd24008">
    <property type="entry name" value="ASKHA_NBD_GLK"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="3.30.420.40:FF:000045">
    <property type="entry name" value="Glucokinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="3.40.367.20:FF:000002">
    <property type="entry name" value="Glucokinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.30.420.40">
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.40.367.20">
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_00524">
    <property type="entry name" value="Glucokinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR043129">
    <property type="entry name" value="ATPase_NBD"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR050201">
    <property type="entry name" value="Bacterial_glucokinase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR003836">
    <property type="entry name" value="Glucokinase"/>
  </dbReference>
  <dbReference type="NCBIfam" id="TIGR00749">
    <property type="entry name" value="glk"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF001414">
    <property type="entry name" value="PRK00292.1-1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF001416">
    <property type="entry name" value="PRK00292.1-3"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR47690">
    <property type="entry name" value="GLUCOKINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR47690:SF1">
    <property type="entry name" value="GLUCOKINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF02685">
    <property type="entry name" value="Glucokinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF53067">
    <property type="entry name" value="Actin-like ATPase domain"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0067">ATP-binding</keyword>
  <keyword id="KW-0963">Cytoplasm</keyword>
  <keyword id="KW-0324">Glycolysis</keyword>
  <keyword id="KW-0418">Kinase</keyword>
  <keyword id="KW-0547">Nucleotide-binding</keyword>
  <keyword id="KW-0808">Transferase</keyword>
  <feature type="chain" id="PRO_1000127705" description="Glucokinase">
    <location>
      <begin position="1"/>
      <end position="321"/>
    </location>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="8"/>
      <end position="13"/>
    </location>
    <ligand>
      <name>ATP</name>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
    </ligand>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_00524"/>
    </source>
  </evidence>
  <sequence length="321" mass="34723" checksum="2D6CD956641A3823" modified="2008-12-16" version="1">MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFTAVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGHVDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDITERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>