B4TQT9 BIOB_SALSV Biotin synthase 2.8.1.6 bioB SeSA_A0944 Salmonella schwarzengrund (strain CVM19633) Bacteria Pseudomonadati Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] CVM19633 Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. (4R,5S)-dethiobiotin + (sulfur carrier)-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (sulfur carrier)-H + biotin + 2 5'-deoxyadenosine + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] [4Fe-4S] cluster Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. [2Fe-2S] cluster Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine. Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. Homodimer. Belongs to the radical SAM superfamily. Biotin synthase family. 2Fe-2S 4Fe-4S Biotin biosynthesis Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine Transferase [4Fe-4S] cluster 4Fe-4S-S-AdoMet [4Fe-4S] cluster 4Fe-4S-S-AdoMet [4Fe-4S] cluster 4Fe-4S-S-AdoMet [2Fe-2S] cluster [2Fe-2S] cluster [2Fe-2S] cluster [2Fe-2S] cluster MARHPRWTLSQVTELFEKPLLELLFEAQQIHRQHFDPQQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLDSARKAKNAGSTRFCMGAAWKNPHERDMPYLEQIVQGVKAMGLETCMTLGMLNESQAQRLANAGLDYYNHNLDTSPEFYGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVTDRAGLLLQLANLPTPPESVPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPAEDKDLQLFRKLGLNPQQTRVLAGDNEQQQRLEQTLMTPDTDDYYNAAAL Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License