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<entry dataset="Swiss-Prot" created="2009-04-14" modified="2026-06-10" version="81" xmlns="http://uniprot.org/uniprot">
  <accession>B4T9N8</accession>
  <name>HIS4_SALHS</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
      <ecNumber evidence="1">5.3.1.16</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">hisA</name>
    <name type="ordered locus">SeHA_C2302</name>
  </gene>
  <organism>
    <name type="scientific">Salmonella heidelberg (strain SL476)</name>
    <dbReference type="NCBI Taxonomy" id="454169"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Enterobacterales</taxon>
      <taxon>Enterobacteriaceae</taxon>
      <taxon>Salmonella</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2011" name="J. Bacteriol." volume="193" first="3556" last="3568">
      <title>Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution.</title>
      <authorList>
        <person name="Fricke W.F."/>
        <person name="Mammel M.K."/>
        <person name="McDermott P.F."/>
        <person name="Tartera C."/>
        <person name="White D.G."/>
        <person name="Leclerc J.E."/>
        <person name="Ravel J."/>
        <person name="Cebula T.A."/>
      </authorList>
      <dbReference type="PubMed" id="21602358"/>
      <dbReference type="DOI" id="10.1128/jb.00297-11"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>SL476</strain>
    </source>
  </reference>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
      <dbReference type="Rhea" id="RHEA:15469"/>
      <dbReference type="ChEBI" id="CHEBI:58435"/>
      <dbReference type="ChEBI" id="CHEBI:58525"/>
      <dbReference type="EC" id="5.3.1.16"/>
    </reaction>
  </comment>
  <comment type="pathway">
    <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
  </comment>
  <comment type="subcellular location">
    <subcellularLocation>
      <location evidence="1">Cytoplasm</location>
    </subcellularLocation>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the HisA/HisF family.</text>
  </comment>
  <dbReference type="EC" id="5.3.1.16" evidence="1"/>
  <dbReference type="EMBL" id="CP001120">
    <property type="protein sequence ID" value="ACF67763.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_000586409.1">
    <property type="nucleotide sequence ID" value="NC_011083.1"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="B4T9N8"/>
  <dbReference type="SMR" id="B4T9N8"/>
  <dbReference type="KEGG" id="seh:SeHA_C2302"/>
  <dbReference type="HOGENOM" id="CLU_048577_1_2_6"/>
  <dbReference type="UniPathway" id="UPA00031">
    <property type="reaction ID" value="UER00009"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005737">
    <property type="term" value="C:cytoplasm"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-SubCell"/>
  </dbReference>
  <dbReference type="GO" id="GO:0003949">
    <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0000105">
    <property type="term" value="P:L-histidine biosynthetic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0000162">
    <property type="term" value="P:L-tryptophan biosynthetic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="CDD" id="cd04732">
    <property type="entry name" value="HisA"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="3.20.20.70:FF:000009">
    <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.20.20.70">
    <property type="entry name" value="Aldolase class I"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_01014">
    <property type="entry name" value="HisA"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR013785">
    <property type="entry name" value="Aldolase_TIM"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR006062">
    <property type="entry name" value="His_biosynth"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR023016">
    <property type="entry name" value="HisA/PriA"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR006063">
    <property type="entry name" value="HisA_bact_arch"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR044524">
    <property type="entry name" value="Isoase_HisA-like"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR011060">
    <property type="entry name" value="RibuloseP-bd_barrel"/>
  </dbReference>
  <dbReference type="NCBIfam" id="TIGR00007">
    <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR43090">
    <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR43090:SF2">
    <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF00977">
    <property type="entry name" value="His_biosynth"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF51366">
    <property type="entry name" value="Ribulose-phoshate binding barrel"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
  <keyword id="KW-0963">Cytoplasm</keyword>
  <keyword id="KW-0368">Histidine biosynthesis</keyword>
  <keyword id="KW-0413">Isomerase</keyword>
  <feature type="chain" id="PRO_1000190553" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
    <location>
      <begin position="1"/>
      <end position="245"/>
    </location>
  </feature>
  <feature type="active site" description="Proton acceptor" evidence="1">
    <location>
      <position position="7"/>
    </location>
  </feature>
  <feature type="active site" description="Proton donor" evidence="1">
    <location>
      <position position="129"/>
    </location>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_01014"/>
    </source>
  </evidence>
  <sequence length="245" mass="26089" checksum="20CF123F43DCE925" modified="2008-09-23" version="1">MIIPALDLIDGTVVRLHQGDYARQRDYGNDPLPRLQDYAAQGAGVLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRTEEDVAALLKAGVARVVIGSTAVKSPDVVKGWFERFGAQALVLALDVRIDEHGTKQVAVSGWQENSGVSLEQLVETYLPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQIAFQSSGGIGDIDDIAALRGTGVRGVIVGRALLEGKFTVKEAIQCWQNV</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>