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<entry dataset="Swiss-Prot" created="2009-04-14" modified="2026-06-10" version="78" xmlns="http://uniprot.org/uniprot">
  <accession>B4T9K8</accession>
  <name>ALLA_SALHS</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">Ureidoglycolate lyase</fullName>
      <ecNumber evidence="1">4.3.2.3</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">Ureidoglycolatase</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">allA</name>
    <name type="ordered locus">SeHA_C0623</name>
  </gene>
  <organism>
    <name type="scientific">Salmonella heidelberg (strain SL476)</name>
    <dbReference type="NCBI Taxonomy" id="454169"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Enterobacterales</taxon>
      <taxon>Enterobacteriaceae</taxon>
      <taxon>Salmonella</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2011" name="J. Bacteriol." volume="193" first="3556" last="3568">
      <title>Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution.</title>
      <authorList>
        <person name="Fricke W.F."/>
        <person name="Mammel M.K."/>
        <person name="McDermott P.F."/>
        <person name="Tartera C."/>
        <person name="White D.G."/>
        <person name="Leclerc J.E."/>
        <person name="Ravel J."/>
        <person name="Cebula T.A."/>
      </authorList>
      <dbReference type="PubMed" id="21602358"/>
      <dbReference type="DOI" id="10.1128/jb.00297-11"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>SL476</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Catalyzes the catabolism of the allantoin degradation intermediate (S)-ureidoglycolate, generating urea and glyoxylate. Involved in the utilization of allantoin as nitrogen source.</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>(S)-ureidoglycolate = urea + glyoxylate</text>
      <dbReference type="Rhea" id="RHEA:11304"/>
      <dbReference type="ChEBI" id="CHEBI:16199"/>
      <dbReference type="ChEBI" id="CHEBI:36655"/>
      <dbReference type="ChEBI" id="CHEBI:57296"/>
      <dbReference type="EC" id="4.3.2.3"/>
    </reaction>
  </comment>
  <comment type="cofactor">
    <cofactor evidence="1">
      <name>Ni(2+)</name>
      <dbReference type="ChEBI" id="CHEBI:49786"/>
    </cofactor>
  </comment>
  <comment type="pathway">
    <text evidence="1">Nitrogen metabolism; (S)-allantoin degradation.</text>
  </comment>
  <comment type="subunit">
    <text evidence="1">Homodimer.</text>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the ureidoglycolate lyase family.</text>
  </comment>
  <dbReference type="EC" id="4.3.2.3" evidence="1"/>
  <dbReference type="EMBL" id="CP001120">
    <property type="protein sequence ID" value="ACF66432.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_000764659.1">
    <property type="nucleotide sequence ID" value="NC_011083.1"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="B4T9K8"/>
  <dbReference type="SMR" id="B4T9K8"/>
  <dbReference type="KEGG" id="seh:SeHA_C0623"/>
  <dbReference type="HOGENOM" id="CLU_070848_1_1_6"/>
  <dbReference type="UniPathway" id="UPA00395"/>
  <dbReference type="GO" id="GO:0004848">
    <property type="term" value="F:ureidoglycolate hydrolase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="GO" id="GO:0050385">
    <property type="term" value="F:ureidoglycolate lyase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0000256">
    <property type="term" value="P:allantoin catabolic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0006145">
    <property type="term" value="P:purine nucleobase catabolic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="CDD" id="cd20298">
    <property type="entry name" value="cupin_UAH"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="2.60.120.480:FF:000001">
    <property type="entry name" value="Ureidoglycolate lyase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="2.60.120.480">
    <property type="entry name" value="Ureidoglycolate hydrolase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_00616">
    <property type="entry name" value="Ureidogly_lyase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR011051">
    <property type="entry name" value="RmlC_Cupin_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR047233">
    <property type="entry name" value="UAH_cupin"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR007247">
    <property type="entry name" value="Ureidogly_lyase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR023525">
    <property type="entry name" value="Ureidogly_lyase_bac"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR024060">
    <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF002948">
    <property type="entry name" value="PRK03606.1-1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF009932">
    <property type="entry name" value="PRK13395.1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR21221">
    <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR21221:SF1">
    <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF04115">
    <property type="entry name" value="Ureidogly_lyase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PIRSF" id="PIRSF017306">
    <property type="entry name" value="Ureidogly_hydro"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF51182">
    <property type="entry name" value="RmlC-like cupins"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0456">Lyase</keyword>
  <keyword id="KW-0659">Purine metabolism</keyword>
  <feature type="chain" id="PRO_1000130426" description="Ureidoglycolate lyase">
    <location>
      <begin position="1"/>
      <end position="160"/>
    </location>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_00616"/>
    </source>
  </evidence>
  <sequence length="160" mass="18124" checksum="A4579B316E801F50" modified="2008-09-23" version="1">MKLEVLPLDQKTFSAYGDVIETQERDFFHINNGLVERYHDLAKVEVLEQDRTLISINRAQPAAMPIVVHELERHPLGTQAFVPMNGEAFVVIVALGDDKPDLSTLRAFISNGRQGVNYHRNVWHHPLFAWQTVTDFLTVDRGGSDNCDVESIPTHELCFA</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>