ID   B4RAS9_PHEZH            Unreviewed;      1085 AA.
AC   B4RAS9;
DT   23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   23-SEP-2008, sequence version 1.
DT   02-SEP-2026, entry version 92.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273, ECO:0000256|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|HAMAP-Rule:MF_01902};
GN   Name=dnaE-1 {ECO:0000313|EMBL:ACG79677.1};
GN   Synonyms=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN   OrderedLocusNames=PHZ_c3268 {ECO:0000313|EMBL:ACG79677.1};
OS   Phenylobacterium zucineum (strain HLK1).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Caulobacterales; Caulobacteraceae; Phenylobacterium.
OX   NCBI_TaxID=450851 {ECO:0000313|EMBL:ACG79677.1, ECO:0000313|Proteomes:UP000001868};
RN   [1] {ECO:0000313|EMBL:ACG79677.1, ECO:0000313|Proteomes:UP000001868}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HLK1 {ECO:0000313|EMBL:ACG79677.1,
RC   ECO:0000313|Proteomes:UP000001868};
RX   PubMed=18700039; DOI=10.1186/1471-2164-9-386;
RA   Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.;
RT   "Complete genome of Phenylobacterium zucineum - a novel facultative
RT   intracellular bacterium isolated from human erythroleukemia cell line
RT   K562.";
RL   BMC Genomics 9:386-386(2008).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) +
CC         diphosphate; Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-
CC         COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112;
CC         EC=2.7.7.7; Evidence={ECO:0000256|ARBA:ARBA00049244,
CC         ECO:0000256|HAMAP-Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC       ECO:0000256|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000256|ARBA:ARBA00007391, ECO:0000256|HAMAP-
CC       Rule:MF_01902}.
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DR   EMBL; CP000747; ACG79677.1; -; Genomic_DNA.
DR   RefSeq; WP_012523815.1; NC_011144.1.
DR   AlphaFoldDB; B4RAS9; -.
DR   STRING; 450851.PHZ_c3268; -.
DR   KEGG; pzu:PHZ_c3268; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OrthoDB; 9803237at2; -.
DR   Proteomes; UP000001868; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DnaE2.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   NCBIfam; TIGR00594; polc; 1.
DR   NCBIfam; NF004225; PRK05672.1; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW   DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW   Rule:MF_01902};
KW   DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW   Rule:MF_01902};
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW   Rule:MF_01902};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW   ECO:0000256|HAMAP-Rule:MF_01902};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW   Rule:MF_01902}; Reference proteome {ECO:0000313|Proteomes:UP000001868};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW   Rule:MF_01902}.
FT   DOMAIN          5..77
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
FT   REGION          808..830
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        816..827
FT                   /note="Gly residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1085 AA;  120532 MW;  1A001ED850BFB71C CRC64;
     MTRYAELQAV TNFSFLRGAS HPGDLVVAAD ALGMEAVGIC DHNSLAGVVR AWSAMRQLHE
     GGSAVRALTG CRLDFVDGSP SLLVYPSDRE AYGRLTRLLT LGQRRSEKGA CTLRLADFLD
     HAEGQLVLAV PPDRLDAAFG DALKRLAGDL PDVWLAAARR YGARDLKRLS HLQALAEASG
     APMVATNDVL YHGPERRPLQ DVLTCIREKC TIHEAGLRLQ ANAERHLKAP DEMARLFARF
     PGAVERTVEI AGRIGFDLSQ LRYEYPDEPV PPGKTAIQHL RDLAWAGAAW RYPEGVPEKV
     RKLVAHELDL IEKLDFPNYF LTVHDIVRYA RDELKILCQG RGSAANSCVC FCLGITAVDP
     TKEDQDLLFS RFISENRGEP PDIDVDFEHE RREEVMQYVY DRYGRDRAAI VATVIHYRPR
     MAIRQVGKAL GLTEDVTAAL ANTVWGSYGD GVPDQHIRQC GLDPDAPEIR RATALAQELL
     GFPRHLSQHV GGYVLTKRRL DETVPIGNAA MKDRTFIEWD KDDIDALGLM KVDVLALGML
     TALRKSFELL PPLPDGTRIT DIAHVPQEDP GVYDMLCRAD SVGVFQVESR AQMSMLPRLK
     PRRFYDLVVQ VAIVRPGPIQ GDMVHPYLKR RQGLEPAEFP APSPAHGPPD ELRAVLGKTY
     GVPLFQEQAM RLAIEAAKFT DSEADGLRRS MATFRANGRL WEYREKFIRG MTARGYDEGF
     AQRCFKQIEG FGSYGFPESH AISFALLVYA SAWVKRHHPD VFCVAMLNSQ PMGFYQPAQL
     VRDAREHGVE VRPPDVMASV WDCTLEETER ASSSPVGGGG PRSGGGGLRR RPVRLGFRQI
     KGFRQDDAER LVRAREAGCR TIQDFAQAAG LSRRALELLA EADAFRSLSM DRRQALWAVK
     GLAGELNALE AAPLLARQSA KEQQVLLPFM SPAQHVAEDY RTTSLSLKAH PVGFFREDLN
     RMGAVRCGTL KDARGGRRLS VGGLVLVRQR PGTAKGVVFL TLEDETGIAN IVVWKDVFEA
     NRRTVMTAAF LVVHGRVQSE SGVVHVVAER FTDLSERLSE MREDTAPIET RSKVSARLIR
     SRDFH
//