ID   B4EVM3_PROMH            Unreviewed;       287 AA.
AC   B4EVM3;
DT   23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   23-SEP-2008, sequence version 1.
DT   02-SEP-2026, entry version 99.
DE   SubName: Full=Lipase {ECO:0000313|EMBL:CAR42190.1};
DE            EC=3.1.1.3 {ECO:0000313|EMBL:CAR42190.1};
GN   OrderedLocusNames=PMI0999 {ECO:0000313|EMBL:CAR42190.1};
OS   Proteus mirabilis (strain HI4320).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Morganellaceae; Proteus.
OX   NCBI_TaxID=529507 {ECO:0000313|EMBL:CAR42190.1, ECO:0000313|Proteomes:UP000008319};
RN   [1] {ECO:0000313|EMBL:CAR42190.1, ECO:0000313|Proteomes:UP000008319}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI4320 {ECO:0000313|EMBL:CAR42190.1,
RC   ECO:0000313|Proteomes:UP000008319};
RX   PubMed=18375554; DOI=10.1128/JB.01981-07;
RA   Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA   Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA   Parkhill J., Mobley H.L.;
RT   "Complete genome sequence of uropathogenic Proteus mirabilis, a master of
RT   both adherence and motility.";
RL   J. Bacteriol. 190:4027-4037(2008).
RN   [2] {ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH CA(2+).
RX   PubMed=23300806; DOI=10.1371/journal.pone.0052890;
RA   Korman T.P., Bowie J.U.;
RT   "Crystal structure of Proteus mirabilis lipase, a novel lipase from the
RT   Proteus/psychrophilic subfamily of lipase family I.1.";
RL   PLoS ONE 7:e52890-e52890(2012).
RN   [3] {ECO:0007829|PDB:4HS9}
RP   X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH CA(2+).
RX   PubMed=23648063; DOI=10.1186/1754-6834-6-70;
RA   Korman T.P., Sahachartsiri B., Charbonneau D.M., Huang G.L., Beauregard M.,
RA   Bowie J.U.;
RT   "Dieselzymes: development of a stable and methanol tolerant lipase for
RT   biodiesel production by directed evolution.";
RL   Biotechnol. Biofuels 6:70-70(2013).
RN   [4] {ECO:0007829|PDB:3W9U}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
RA   Yuan Y.A.;
RT   "Crystal structure of Lipk107.";
RL   Submitted (APR-2013) to the PDB data bank.
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DR   EMBL; AM942759; CAR42190.1; -; Genomic_DNA.
DR   RefSeq; WP_004247922.1; NC_010554.1.
DR   PDB; 3W9U; X-ray; 2.00 A; A=1-287.
DR   PDB; 4GW3; X-ray; 2.00 A; A=1-287.
DR   PDB; 4GXN; X-ray; 2.20 A; A=1-287.
DR   PDB; 4HS9; X-ray; 1.80 A; A=1-287.
DR   AlphaFoldDB; B4EVM3; -.
DR   SMR; B4EVM3; -.
DR   ESTHER; promi-c2lfd0; Bacterial_lip_FamI.1.
DR   DNASU; 6803666; -.
DR   EnsemblBacteria; CAR42190; CAR42190; PMI0999.
DR   GeneID; 6803666; -.
DR   KEGG; pmr:PMI0999; -.
DR   eggNOG; COG1075; Bacteria.
DR   HOGENOM; CLU_062016_0_0_6; -.
DR   EvolutionaryTrace; B4EVM3; -.
DR   Proteomes; UP000008319; Chromosome.
DR   GO; GO:0004806; F:triacylglycerol lipase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR029058; AB_hydrolase_fold.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   PDBsum; 3W9U; -.
DR   PDBsum; 4GW3; -.
DR   PDBsum; 4GXN; -.
DR   PDBsum; 4HS9; -.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:3W9U, ECO:0007829|PDB:4GW3};
KW   Calcium {ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN};
KW   Hydrolase {ECO:0000313|EMBL:CAR42190.1};
KW   Metal-binding {ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008319}.
FT   DOMAIN          5..182
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000259|Pfam:PF00561"
FT   BINDING         51
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:4GW3"
FT   BINDING         210
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN"
FT   BINDING         213
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN"
FT   BINDING         256
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN"
FT   BINDING         260
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4GW3, ECO:0007829|PDB:4GXN"
FT   BINDING         264
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4GXN, ECO:0007829|PDB:4HS9"
SQ   SEQUENCE   287 AA;  31647 MW;  03B81B0827F709C5 CRC64;
     MSTKYPIVLV HGLAGFNEIV GFPYFYGIAD ALRQDGHQVF TASLSAFNSN EVRGKQLWQF
     VQTLLQETQA KKVNFIGHSQ GPLACRYVAA NYPDSVASVT SINGVNHGSE IADLYRRIMR
     KDSIPEYIVE KVLNAFGTII STFSGHRGDP QDAIAALESL TTEQVTEFNN KYPQALPKTP
     GGEGDEIVNG VHYYCFGSYI QGLIAGEKGN LLDPTHAAMR VLNTFFTEKQ NDGLVGRSSM
     RLGKLIKDDY AQDHIDMVNQ VAGLVGYNED IVAIYTQHAK YLASKQL
//