ID   B4EHT1_BURCJ            Unreviewed;       332 AA.
AC   B4EHT1;
DT   23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   23-SEP-2008, sequence version 1.
DT   02-SEP-2026, entry version 79.
DE   RecName: Full=Isoaspartyl peptidase {ECO:0000256|ARBA:ARBA00069124};
GN   ORFNames=BCAM1291 {ECO:0000313|EMBL:CAR55147.1};
OS   Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 /
OS   NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC   Burkholderiales; Burkholderiaceae; Burkholderia;
OC   Burkholderia cepacia complex.
OX   NCBI_TaxID=216591 {ECO:0000313|EMBL:CAR55147.1, ECO:0000313|Proteomes:UP000001035};
RN   [1] {ECO:0000313|EMBL:CAR55147.1, ECO:0000313|Proteomes:UP000001035}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 /
RC   CF5610 {ECO:0000313|Proteomes:UP000001035};
RX   PubMed=18931103; DOI=10.1128/JB.01230-08;
RA   Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D.,
RA   Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S.,
RA   Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K.,
RA   Scott P., Walker D., White B., Rose H., Iversen P., Mil-Homens D.,
RA   Rocha E.P., Fialho A.M., Baldwin A., Dowson C., Barrell B.G., Govan J.R.,
RA   Vandamme P., Hart C.A., Mahenthiralingam E., Parkhill J.;
RT   "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of
RT   cystic fibrosis patients.";
RL   J. Bacteriol. 191:261-277(2009).
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DR   EMBL; AM747721; CAR55147.1; -; Genomic_DNA.
DR   RefSeq; WP_006482631.1; NC_011001.1.
DR   AlphaFoldDB; B4EHT1; -.
DR   GeneID; 56561874; -.
DR   KEGG; bcj:BCAM1291; -.
DR   eggNOG; COG1446; Bacteria.
DR   HOGENOM; CLU_021603_1_2_4; -.
DR   BioCyc; BCEN216591:G1G1V-5299-MONOMER; -.
DR   Proteomes; UP000001035; Chromosome 2.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:UniProtKB-ARBA.
DR   CDD; cd04701; Asparaginase_2; 1.
DR   FunFam; 3.60.20.30:FF:000001; Isoaspartyl peptidase/L-asparaginase; 1.
DR   Gene3D; 3.60.20.30; (Glycosyl)asparaginase; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR000246; Peptidase_T2.
DR   PANTHER; PTHR10188; L-ASPARAGINASE; 1.
DR   PANTHER; PTHR10188:SF6; N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE; 1.
DR   Pfam; PF01112; Asparaginase_2; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   4: Predicted;
KW   Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CAR55147.1};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001035}.
FT   ACT_SITE        195
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-1"
FT   BINDING         223..226
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-2"
FT   BINDING         246..249
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-2"
FT   SITE            194..195
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-3"
SQ   SEQUENCE   332 AA;  34248 MW;  923BBD371A58D630 CRC64;
     MTSPAIVAIH GGAGTILREA MDTDTERRYR AELTAILQAA QRVLADGGSA LDAVTVAVRM
     LEDCPLFNAG RGAVYTAEGK HELDAAVMDG ATLGAGAICC ATRVRNPVLA ARRVMEASEH
     VLFAGAGADA FAAAQGLELA EPGYFDTESR HAQWVKARAA AGAMLDHDAA TFVFGQSQQQ
     PQPAEPLDPD RKHGTVGAVA CDLNGHIAAA TSTGGITNKQ PGRVGDSPII GAGCYADDAT
     CAVSSTGTGE MFMRLATAYD VAAQIAYRGA SLADAAHDVV MNKLPRLAGR GGIIAVDAQG
     NVAMPFNTEG MYRGYARVGE APVVGIYRDD AA
//