ID BCUSY_MAGGA Reviewed; 550 AA. AC B3TPQ6; DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot. DT 02-SEP-2008, sequence version 1. DT 05-FEB-2025, entry version 47. DE RecName: Full=Beta-cubebene synthase; DE Short=Mg25; DE EC=4.2.3.128; OS Magnolia grandiflora (Southern magnolia). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Magnoliidae; Magnoliales; Magnoliaceae; OC Magnolia. OX NCBI_TaxID=3406; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, RP BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND TISSUE SPECIFICITY. RX PubMed=18467455; DOI=10.1104/pp.108.115824; RA Lee S., Chappell J.; RT "Biochemical and genomic characterization of terpene synthases in Magnolia RT grandiflora."; RL Plant Physiol. 147:1017-1033(2008). CC -!- FUNCTION: Sesquiterpene synthase converting farnesyl diphosphate into CC beta-cubebene (24.5%), alpha-muurolene (19.3%), delta-cadinol (18.6%), CC delta-elemene (16.0%), tau-muurolene (10.8%), and beta-elemene (10.8%). CC No activity with geranyl diphosphate or geranylgeranyl diphosphate. CC {ECO:0000269|PubMed:18467455}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6E)-farnesyl diphosphate = beta-cubebene + diphosphate; CC Xref=Rhea:RHEA:32019, ChEBI:CHEBI:10363, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:175763; EC=4.2.3.128; CC Evidence={ECO:0000269|PubMed:18467455}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000305|PubMed:18467455}; CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000305|PubMed:18467455}; CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC Kinetic parameters: CC KM=1.07 uM for farnesyl diphosphate {ECO:0000269|PubMed:18467455}; CC Note=kcat is 0.35 x 10(-3) sec(-1) for farnesyl diphosphate.; CC pH dependence: CC Optimum pH is 7.5. {ECO:0000269|PubMed:18467455}; CC Temperature dependence: CC Optimum temperature is 24-26 degrees Celsius. CC {ECO:0000269|PubMed:18467455}; CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. CC -!- TISSUE SPECIFICITY: Expressed in young developing leaves and in CC stamens. Not detected in tepals and carpels. CC {ECO:0000269|PubMed:18467455}. CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for CC the catalytic activity, presumably through binding to Mg(2+). CC {ECO:0000250}. CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; EU366429; ACC66281.1; -; Genomic_DNA. DR AlphaFoldDB; B3TPQ6; -. DR SMR; B3TPQ6; -. DR KEGG; ag:ACC66281; -. DR BioCyc; MetaCyc:MONOMER-14947; -. DR BRENDA; 4.2.3.128; 13170. DR SABIO-RK; B3TPQ6; -. DR UniPathway; UPA00213; -. DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro. DR GO; GO:0010334; F:sesquiterpene synthase activity; IDA:UniProtKB. DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro. DR GO; GO:0045338; P:farnesyl diphosphate metabolic process; IDA:UniProtKB. DR GO; GO:0051762; P:sesquiterpene biosynthetic process; IDA:UniProtKB. DR CDD; cd00684; Terpene_cyclase_plant_C1; 1. DR FunFam; 1.10.600.10:FF:000007; Isoprene synthase, chloroplastic; 1. DR FunFam; 1.50.10.130:FF:000001; Isoprene synthase, chloroplastic; 1. DR Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1. DR Gene3D; 1.50.10.130; Terpene synthase, N-terminal domain; 1. DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf. DR InterPro; IPR034741; Terpene_cyclase-like_1_C. DR InterPro; IPR044814; Terpene_cyclase_plant_C1. DR InterPro; IPR001906; Terpene_synth_N. DR InterPro; IPR036965; Terpene_synth_N_sf. DR InterPro; IPR050148; Terpene_synthase-like. DR InterPro; IPR005630; Terpene_synthase_metal-bd. DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase. DR PANTHER; PTHR31225:SF93; ALPHA-HUMULENE_(-)-(E)-BETA-CARYOPHYLLENE SYNTHASE; 1. DR PANTHER; PTHR31225; OS04G0344100 PROTEIN-RELATED; 1. DR Pfam; PF01397; Terpene_synth; 1. DR Pfam; PF03936; Terpene_synth_C; 1. DR SFLD; SFLDS00005; Isoprenoid_Synthase_Type_I; 1. DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1. DR SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 1. DR SUPFAM; SSF48576; Terpenoid synthases; 1. PE 1: Evidence at protein level; KW Lyase; Magnesium; Metal-binding. FT CHAIN 1..550 FT /note="Beta-cubebene synthase" FT /id="PRO_0000419800" FT MOTIF 303..307 FT /note="DDXXD motif" FT BINDING 303 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 303 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 307 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 307 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 447 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="3" FT /evidence="ECO:0000250" FT BINDING 455 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="3" FT /evidence="ECO:0000250" SQ SEQUENCE 550 AA; 63421 MW; BEC626551B7BECF6 CRC64; MDSPTTQRPN MEIGRAFVNY HPSIWGEHFI AASPDVMRLD AHKGRGEELK EVVRNMFSTV NDPLLKMNLI DAIQRLGVAY HFEMEIDKAL GQMYDDHING KDDGFDLQTL ALQFRLLRQQ GYNVSSGVFA KFKDDEGNFS SILSKDTHGL LSLYEAAFLG THGDDILDEA ITFTTVHLKS TLPHVSAPLT KLVELALEIP LHRRMERLQT RFYISIYEED RERNDVLLEF SKLEFLRLQS LHQRELRDIS LWWKEMDLLA KLPFTRDRVL EGYFWTVGVY FEPHYSRARM IMTKMIAFAT VMDDTYDVYG TLEELELLTA TIERWNRGDM DQLPDYMKVI FIALLDGVDA TEDDLTGEGK SYRIYYLKEA VKDLAKAYLA EARWVSSGYV PTSEEYMKVA LISAVYPMLF VAFLIGMDEV VTKEVLEWAI HMPTMLRTCS IVARLMDDIP SNKLEQERKH VSSSVECYMK EHGTSYHESI QKLREMVASG WKDINKECLK PTPVPTAVIN VILNFTRVLE IIYQHRDGYT DASVETKEHI ASLFVDPIPL //