ID   ZDH12_ARATH             Reviewed;         338 AA.
AC   B3DN87; Q9LER6;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   02-SEP-2026, entry version 102.
DE   RecName: Full=Probable protein S-acyltransferase 1;
DE            EC=2.3.1.225;
DE   AltName: Full=Probable palmitoyltransferase At3g56920;
DE   AltName: Full=Zinc finger DHHC domain-containing protein At3g56920;
GN   Name=PAT01; OrderedLocusNames=At3g56920; ORFNames=T8M16_250;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND FUNCTION.
RA   Hemsley P.A., Taylor L., Grierson C.S.;
RT   "S-acylation: dynamic control of plant development and sigalling by lipid
RT   modification of proteins.";
RL   (In) Proceedings of the 18th international conference on Arabidopsis
RL   research, abstract#139, Beijing (2007).
RN   [6]
RP   SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=22968831; DOI=10.1104/pp.112.203968;
RA   Batistic O.;
RT   "Genomics and localization of the Arabidopsis DHHC-cysteine-rich domain S-
RT   acyltransferase protein family.";
RL   Plant Physiol. 160:1597-1612(2012).
CC   -!- FUNCTION: Palmitoyl acyltransferase. {ECO:0000250, ECO:0000269|Ref.5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-
CC         cysteinyl-[protein] + CoA; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC         COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC         EC=2.3.1.225;
CC   -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC00755.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL390921; CAC00755.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE79586.1; -; Genomic_DNA.
DR   EMBL; OZ408685; CAO7044742.1; -; Genomic_DNA.
DR   EMBL; BT032875; ACD89065.1; -; mRNA.
DR   PIR; T51280; T51280.
DR   RefSeq; NP_191251.2; NM_115551.3.
DR   AlphaFoldDB; B3DN87; -.
DR   SMR; B3DN87; -.
DR   FunCoup; B3DN87; 1624.
DR   iPTMnet; B3DN87; -.
DR   PaxDb; 3702-B3DN87; -.
DR   ProteomicsDB; 242931; -.
DR   GeneID; 824859; -.
DR   KEGG; ath:AT3G56920; -.
DR   Araport; AT3G56920; -.
DR   TAIR; AT3G56920; -.
DR   eggNOG; KOG1311; Eukaryota.
DR   HOGENOM; CLU_018741_7_1_1; -.
DR   InParanoid; B3DN87; -.
DR   OMA; ISHRHPF; -.
DR   PhylomeDB; B3DN87; -.
DR   BRENDA; 2.3.1.225; 399.
DR   PRO; PR:B3DN87; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR   InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR   InterPro; IPR039859; PFA4/ZDH16/20/ERF2-like.
DR   PANTHER; PTHR22883:SF284; PROTEIN S-ACYLTRANSFERASE 1-RELATED; 1.
DR   PANTHER; PTHR22883; ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN; 1.
DR   Pfam; PF01529; DHHC; 1.
DR   PROSITE; PS50216; DHHC; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Endosome; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..338
FT                   /note="Probable protein S-acyltransferase 1"
FT                   /id="PRO_0000363598"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          142..192
FT                   /note="DHHC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT   REGION          319..338
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        320..338
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        172
FT                   /note="S-palmitoyl cysteine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   338 AA;  38669 MW;  7002A20BC3B9D646 CRC64;
     MSSQNLERQR IYQVWPAKNK FYCGGRLVFG PDASSLLLTT CMIGGPAIAF SIRMAYLISH
     RHPFFHSLTL IGAILLTFMA FTFLFLTSSR DPGIIPRNKQ VSEAEIPDVT TQSTEWVTSK
     LGSVKLPRTK DVMVNGFTVK VKFCDTCQLY RPPRAFHCSI CNNCVQRFDH HCPWVGQCIA
     LRNYPFFVCF LSCSTLLCIY VFVFSWVSML KVHGEFYVVL ADDLILGVLG LYCFVSVWFV
     GGLTVFHFYL ICTNQTTCEN FRYHYDKKEN PYRKGILENF KELFFAKIPP PLINFRDWSP
     EEEDDVEVGS IASELVRAFG PKDTKMSSGK SDSEARER
//