ID   B1WTP5_CROS5            Unreviewed;       359 AA.
AC   B1WTP5;
DT   20-MAY-2008, integrated into UniProtKB/TrEMBL.
DT   20-MAY-2008, sequence version 1.
DT   18-JUN-2025, entry version 83.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000256|HAMAP-Rule:MF_01840};
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000256|HAMAP-Rule:MF_01840};
DE            EC=1.14.13.81 {ECO:0000256|HAMAP-Rule:MF_01840};
GN   Name=acsF {ECO:0000256|HAMAP-Rule:MF_01840};
GN   OrderedLocusNames=cce_4412 {ECO:0000313|EMBL:ACB53760.1};
OS   Crocosphaera subtropica (strain ATCC 51142 / BH68) (Cyanothece sp. (strain
OS   ATCC 51142)).
OC   Bacteria; Bacillati; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC   Chroococcales; Aphanothecaceae; Crocosphaera; Crocosphaera subtropica.
OX   NCBI_TaxID=43989 {ECO:0000313|EMBL:ACB53760.1, ECO:0000313|Proteomes:UP000001203};
RN   [1] {ECO:0000313|EMBL:ACB53760.1, ECO:0000313|Proteomes:UP000001203}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51142 / BH68 {ECO:0000313|Proteomes:UP000001203};
RX   PubMed=18812508; DOI=10.1073/pnas.0805418105;
RA   Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C.,
RA   Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K.,
RA   Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B.;
RT   "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium
RT   important in the marine nitrogen cycle.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC       biosynthesis. Mediates the cyclase reaction, which results in the
CC       formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC       chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC       (MgPMME). {ECO:0000256|HAMAP-Rule:MF_01840}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 O2 + 2
CC         H(+) = 3,8-divinyl protochlorophyllide a + 3 NADP(+) + 5 H2O;
CC         Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC         Evidence={ECO:0000256|ARBA:ARBA00049231, ECO:0000256|HAMAP-
CC         Rule:MF_01840};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000256|ARBA:ARBA00001962, ECO:0000256|HAMAP-
CC         Rule:MF_01840};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis (light-independent). {ECO:0000256|HAMAP-Rule:MF_01840}.
CC   -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000256|ARBA:ARBA00006550,
CC       ECO:0000256|HAMAP-Rule:MF_01840}.
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DR   EMBL; CP000806; ACB53760.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1WTP5; -.
DR   STRING; 43989.cce_4412; -.
DR   KEGG; cyt:cce_4412; -.
DR   eggNOG; COG1633; Bacteria.
DR   HOGENOM; CLU_048037_0_0_3; -.
DR   UniPathway; UPA00670; -.
DR   Proteomes; UP000001203; Chromosome circular.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0036068; P:light-independent chlorophyll biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd01047; ACSF; 1.
DR   HAMAP; MF_01840; AcsF; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR003251; Rr_diiron-bd_dom.
DR   NCBIfam; TIGR02029; AcsF; 1.
DR   NCBIfam; NF010172; PRK13654.1; 1.
DR   PANTHER; PTHR31053; MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER [OXIDATIVE] CYCLASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR31053:SF2; MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER [OXIDATIVE] CYCLASE, CHLOROPLASTIC; 1.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   SUPFAM; SSF47240; Ferritin-like; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis {ECO:0000256|ARBA:ARBA00023171, ECO:0000256|HAMAP-
KW   Rule:MF_01840};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01840};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW   Rule:MF_01840};
KW   NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|HAMAP-Rule:MF_01840};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, ECO:0000256|HAMAP-
KW   Rule:MF_01840};
KW   Photosynthesis {ECO:0000256|ARBA:ARBA00022531, ECO:0000256|HAMAP-
KW   Rule:MF_01840}; Reference proteome {ECO:0000313|Proteomes:UP000001203}.
FT   DOMAIN          90..223
FT                   /note="Rubrerythrin diiron-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02915"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   359 AA;  42349 MW;  F13E64FD1F0E5C0B CRC64;
     MVMVKTPVKP DQPKPGTKAP SQETLLTPRF YITDFETAAN LSLTEQETEL NAMLTEMRND
     YNRHHFVRNE EFKTGNWDHL DETTREGFIE YLERSCISEF SGFLLFKELS RKLKGRNPIL
     SEMFHLMARD EARHAGFLNK AMADFNLSLD LGKVTKTRTY TFFPLEWVIY TVYLSEKIGY
     WRYIIIFHHL EKHPEKQFYP IFKKFENWCQ DENRHGDIFT ALLRSQPKLW KTWKARLWSR
     FFLLSVFATH TLTVHERASF YESLGLNATE FDKEVIRKTN ETSARAFPSV LDVDHPEFYP
     RLQRCSQRNL KMKEIGESKA PKFVKTLRKL PLMAGIVGDL LRIYLIKPID AEALRGTVR
//