ID   B1MDB5_MYCA9            Unreviewed;       476 AA.
AC   B1MDB5;
DT   29-APR-2008, integrated into UniProtKB/TrEMBL.
DT   29-APR-2008, sequence version 1.
DT   28-JAN-2026, entry version 100.
DE   RecName: Full=Probable malate:quinone oxidoreductase {ECO:0000256|HAMAP-Rule:MF_00212};
DE            EC=1.1.5.4 {ECO:0000256|HAMAP-Rule:MF_00212};
DE   AltName: Full=MQO {ECO:0000256|HAMAP-Rule:MF_00212};
DE   AltName: Full=Malate dehydrogenase [quinone] {ECO:0000256|HAMAP-Rule:MF_00212};
GN   Name=mqo {ECO:0000256|HAMAP-Rule:MF_00212};
GN   OrderedLocusNames=MAB_3159c {ECO:0000313|EMBL:CAM63236.1};
OS   Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CCUG 20993 / CIP
OS   104536 / JCM 13569 / NCTC 13031 / TMC 1543 / L948) (Mycobacterium
OS   abscessus).
OC   Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales;
OC   Mycobacteriaceae; Mycobacteroides; Mycobacteroides abscessus.
OX   NCBI_TaxID=561007 {ECO:0000313|EMBL:CAM63236.1, ECO:0000313|Proteomes:UP000007137};
RN   [1] {ECO:0000313|EMBL:CAM63236.1, ECO:0000313|Proteomes:UP000007137}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19977 / DSM 44196 / CCUG 20993 / CIP 104536 / JCM 13569 /
RC   NCTC 13031 / TMC 1543 / L948 {ECO:0000313|Proteomes:UP000007137};
RX   PubMed=19543527; DOI=10.1371/journal.pone.0005660;
RA   Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M.,
RA   Macheras E., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L.,
RA   Gaillard J.L.;
RT   "Non mycobacterial virulence genes in the genome of the emerging pathogen
RT   Mycobacterium abscessus.";
RL   PLoS ONE 4:E5660-E5660(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + a quinone = a quinol + oxaloacetate;
CC         Xref=Rhea:RHEA:46012, ChEBI:CHEBI:15589, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.1.5.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00001139, ECO:0000256|HAMAP-
CC         Rule:MF_00212};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974, ECO:0000256|HAMAP-
CC         Rule:MF_00212};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle;
CC       oxaloacetate from (S)-malate (quinone route): step 1/1.
CC       {ECO:0000256|ARBA:ARBA00005012, ECO:0000256|HAMAP-Rule:MF_00212}.
CC   -!- SIMILARITY: Belongs to the MQO family. {ECO:0000256|HAMAP-
CC       Rule:MF_00212}.
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DR   EMBL; CU458896; CAM63236.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1MDB5; -.
DR   KEGG; mab:MAB_3159c; -.
DR   UniPathway; UPA00223; UER01008.
DR   Proteomes; UP000007137; Chromosome.
DR   GO; GO:0047545; F:(S)-2-hydroxyglutarate dehydrogenase activity; IEA:TreeGrafter.
DR   GO; GO:0008924; F:L-malate dehydrogenase (quinone) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00212; MQO; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR006231; MQO.
DR   NCBIfam; TIGR01320; mal_quin_oxido; 1.
DR   NCBIfam; NF003606; PRK05257.2-1; 1.
DR   NCBIfam; NF003611; PRK05257.3-2; 1.
DR   PANTHER; PTHR43104; L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43104:SF2; L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR   Pfam; PF06039; Mqo; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|HAMAP-Rule:MF_00212};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630, ECO:0000256|HAMAP-
KW   Rule:MF_00212};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, ECO:0000256|HAMAP-
KW   Rule:MF_00212}; Reference proteome {ECO:0000313|Proteomes:UP000007137};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532, ECO:0000256|HAMAP-
KW   Rule:MF_00212}.
SQ   SEQUENCE   476 AA;  51434 MW;  C68BF1F7D250C0DB CRC64;
     MSATLGALLR LVEPDLSITL IERLDAAASE SSDPWNNAGT GHSALCELNY TPQKADGSID
     ITKAITVNEQ FQVSRQFWAY AVENGVLPDV RGFLNPIPHV SYVHGADKVE FLRKRHEALV
     GNPLFAKMEF IDDDDEFARR LPLMAAGRDF TDPVGLNWSQ DGTDVDFGEL SKQLVGFGVR
     SGTSAVFGTE VRNISRESDG GWLLKLVNGR TGEKRKLKAK FVFVGAGGGA LGLLQKAGIP
     EAKGFGGFPV SGAFLRTNST DLTDGHKAKV YGFPPLGAPP MSAPHLDTRV INGKEWLLFG
     PFAGWSPKFL KMGKVTDLPA SIKPNNLLSM VGVGLTEFKL ATFLLSQLAL TPDDRIDMLR
     EFAPKAQRAD WELITAGQRV QVIRPAKGKG GALEFGTMVL NSADGSIAGL LGASPGASTA
     VPAMLDVMQR CFPDRYSNWM PKLKEMIPSL GISLSDEPSL FGEVWDWGTR VLGLEQ
//