ID   B0ENN7_ENTDS            Unreviewed;       439 AA.
AC   B0ENN7;
DT   26-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   26-FEB-2008, sequence version 1.
DT   04-NOV-2026, entry version 75.
DE   SubName: Full=6-phosphofructokinase, putative {ECO:0000313|EMBL:EDR23864.1};
DE            EC=2.7.1.11 {ECO:0000313|EMBL:EDR23864.1};
GN   ORFNames=EDI_016560 {ECO:0000313|EMBL:EDR23864.1};
OS   Entamoeba dispar (strain ATCC PRA-260 / SAW760).
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=370354 {ECO:0000313|EMBL:EDR23864.1, ECO:0000313|Proteomes:UP000008076};
RN   [1] {ECO:0000313|EMBL:EDR23864.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=SAW760 {ECO:0000313|EMBL:EDR23864.1};
RA   Lorenzi H., Inman J., Schobel S., Amedeo P., Caler E.;
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the phosphorylation of D-fructose 6-phosphate to
CC       fructose 1,6-bisphosphate by ATP, the first committing step of
CC       glycolysis. {ECO:0000256|ARBA:ARBA00002659}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-
CC         bisphosphate + ADP + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634,
CC         ChEBI:CHEBI:456216; EC=2.7.1.11;
CC         Evidence={ECO:0000256|ARBA:ARBA00048070};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
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DR   EMBL; DS550125; EDR23864.1; -; Genomic_DNA.
DR   RefSeq; XP_001739753.1; XM_001739701.1.
DR   AlphaFoldDB; B0ENN7; -.
DR   GeneID; 5884896; -.
DR   KEGG; edi:EDI_016560; -.
DR   VEuPathDB; AmoebaDB:EDI_016560; -.
DR   eggNOG; KOG2440; Eukaryota.
DR   OMA; ACECAGR; -.
DR   OrthoDB; 537915at2759; -.
DR   UniPathway; UPA00109; UER00182.
DR   Proteomes; UP000008076; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-ARBA.
DR   GO; GO:0003872; F:6-phosphofructokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro.
DR   FunFam; 3.40.50.450:FF:000002; ATP-dependent 6-phosphofructokinase; 1.
DR   Gene3D; 3.40.50.450; -; 1.
DR   Gene3D; 3.40.50.460; Phosphofructokinase domain; 1.
DR   InterPro; IPR022953; ATP_PFK.
DR   InterPro; IPR050929; PFKA.
DR   InterPro; IPR000023; Phosphofructokinase_dom.
DR   InterPro; IPR035966; PKF_sf.
DR   InterPro; IPR012004; PyroP-dep_PFK_TP0108.
DR   NCBIfam; NF005301; PRK06830.1; 1.
DR   PANTHER; PTHR45770; ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1; 1.
DR   Pfam; PF00365; PFK; 1.
DR   PIRSF; PIRSF000534; PPi_PFK_TP0108; 1.
DR   PRINTS; PR00476; PHFRCTKINASE.
DR   SUPFAM; SSF53784; Phosphofructokinase; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008076};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EDR23864.1}.
FT   DOMAIN          86..390
FT                   /note="Phosphofructokinase"
FT                   /evidence="ECO:0000259|Pfam:PF00365"
SQ   SEQUENCE   439 AA;  48422 MW;  25D5A8F5E2B5A201 CRC64;
     MTERRHVHQR NDLLIAKKPE EPLPSLEVTS LGECKYENTY ASPEPFVNGM KMSMSAIKID
     GIPVNECEVD LAGPMEKIFF IPERTKVGIV TCGGLCPGLN NVIRGLVMNL QNRYGVKQIY
     GLKYGYEGLV PELSEQIKLD TLTVSDIHQR GGTILGTSRG AQDPKVMAQF LIDNNFNILF
     TLGGDGTLRG ANRINKELRR RGSPIAVVGI PKTIDNDICY TDSTFGFDTA VELAQEAINS
     VHYEAKSAKN GIGIVKLMGR DAGFIALYAS VASGDVNLVL IPEVDTPIEE ILRMTERRLM
     SKGHIVIVIA EGACQNLKPK DLDLGSDKSG NIVHWDAVTY IRQEIDKYLE NKKIEHTIKF
     VDPSYMIRSA PCSAADAHFC LCLANAAVHV AMAGKTGLVI CHHHNNFVSI PIERACYYLK
     RVNPEGPMLS MMKSIEMVE
//