ID   A9WEH2_CHLAA            Unreviewed;       721 AA.
AC   A9WEH2;
DT   05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   05-FEB-2008, sequence version 1.
DT   02-SEP-2026, entry version 83.
DE   SubName: Full=Kojibiose phosphorylase {ECO:0000313|EMBL:ABY35234.1};
DE            EC=2.4.1.230 {ECO:0000313|EMBL:ABY35234.1};
GN   OrderedLocusNames=Caur_2019 {ECO:0000313|EMBL:ABY35234.1};
OS   Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC   Bacteria; Bacillati; Chloroflexota; Chloroflexia; Chloroflexales;
OC   Chloroflexineae; Chloroflexaceae; Chloroflexus.
OX   NCBI_TaxID=324602 {ECO:0000313|EMBL:ABY35234.1, ECO:0000313|Proteomes:UP000002008};
RN   [1] {ECO:0000313|Proteomes:UP000002008}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl
RC   {ECO:0000313|Proteomes:UP000002008};
RX   PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA   Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA   Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA   Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT   "Complete genome sequence of the filamentous anoxygenic phototrophic
RT   bacterium Chloroflexus aurantiacus.";
RL   BMC Genomics 12:334-334(2011).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 65 family.
CC       {ECO:0000256|ARBA:ARBA00006768}.
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DR   EMBL; CP000909; ABY35234.1; -; Genomic_DNA.
DR   RefSeq; WP_012257888.1; NC_010175.1.
DR   AlphaFoldDB; A9WEH2; -.
DR   FunCoup; A9WEH2; 128.
DR   STRING; 324602.Caur_2019; -.
DR   EnsemblBacteria; ABY35234; ABY35234; Caur_2019.
DR   KEGG; cau:Caur_2019; -.
DR   PATRIC; fig|324602.8.peg.2294; -.
DR   eggNOG; COG1554; Bacteria.
DR   HOGENOM; CLU_006285_2_1_0; -.
DR   InParanoid; A9WEH2; -.
DR   Proteomes; UP000002008; Chromosome.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IBA:GO_Central.
DR   GO; GO:0033831; F:kojibiose phosphorylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR   FunFam; 1.50.10.10:FF:000053; Putative glycosyl hydrolase; 1.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.70.98.40; Glycoside hydrolase, family 65, N-terminal domain; 1.
DR   Gene3D; 2.60.420.10; Maltose phosphorylase, domain 3; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR037018; GH65_N.
DR   InterPro; IPR005194; Glyco_hydro_65_C.
DR   InterPro; IPR005195; Glyco_hydro_65_M.
DR   InterPro; IPR005196; Glyco_hydro_65_N.
DR   InterPro; IPR017045; Malt_Pase/Glycosyl_Hdrlase.
DR   PANTHER; PTHR11051; GLYCOSYL HYDROLASE-RELATED; 1.
DR   PANTHER; PTHR11051:SF8; PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE; 1.
DR   Pfam; PF03633; Glyco_hydro_65C; 1.
DR   Pfam; PF03632; Glyco_hydro_65m; 1.
DR   Pfam; PF03636; Glyco_hydro_65N; 1.
DR   PIRSF; PIRSF036289; Glycosyl_hydrolase_malt_phosph; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW   ECO:0000313|EMBL:ABY35234.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002008};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ABY35234.1}.
FT   DOMAIN          5..269
FT                   /note="Glycoside hydrolase family 65 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03636"
FT   DOMAIN          289..667
FT                   /note="Glycoside hydrolase family 65 central catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF03632"
FT   DOMAIN          680..716
FT                   /note="Glycoside hydrolase family 65 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03633"
FT   ACT_SITE        466
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-50"
FT   BINDING         324..325
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
FT   BINDING         577..578
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
SQ   SEQUENCE   721 AA;  81388 MW;  EA722AD526214C3D CRC64;
     MWILAEPSFE PTKQHHKETI FTIGNGYLST RGAFEEGYPN DRRATFIHGV FDDAPIVVTE
     LANAPDWLAT QIVLDGEKFS LASGTVLAYR RELDLRTGVL RREVRWQSPQ GRAATIIFTR
     FASLADQHLL ALRCEIIPEF DGQVELRSAI NGQTDNEGLL HWRPIAQGQL ADGTVFLRTR
     TRKSGIELAL TMRVSGSDTA TTTFWDVENV PTLQQVYQAQ AGVPIVVTKF VGVATSRDDA
     NPLELAHRHL QAATGWDEAL AAQQQVWAKE WERCNVEIEG DEEADLAVRF SIFQLLIAAP
     RHDPRVNIGA KTLSGFGYRG HAFWDTEIFM LPLFIYTAPD IARNLLDYRY LTLPAARAKA
     RAAGYEGAWY AWESADSGEE VTPTWVPDFH DKKKLARVWT GDLAIHISSD VAYAVQQYWQ
     ATGDDAWYIE RGAEIVLDTA KFCAARAEWL NDRGCYGYTD VIGPDEYHDH VNNNAYTNLL
     AQWNLRAALE TLAWLDQHAP QKAAELRQRL DLTPERLQHW QTVAEKMCVN VSPDGLIEQF
     DGFFKLKDVN LADYEPRTKS MHEIFGIEGA NEYQAIKQPD VLMLQYLLRE HYSDSQIRVN
     YNYYTPRTDH TYGSSLGPPI QAIVACQMGD VAEAYEHFIR AARADLRDVR GNAGDGIHAA
     SAGGVWQAVV FGFGGLRVHP DGSWDIQPRL PQHWQRLTFR FTLRGKTYTV TCYPDGRYTT
     A
//