ID   A9QPD2_METI4            Unreviewed;       931 AA.
AC   A9QPD2;
DT   05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   05-FEB-2008, sequence version 1.
DT   04-NOV-2026, entry version 109.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN   ECO:0000313|EMBL:ABX56590.1};
GN   OrderedLocusNames=Minf_1739 {ECO:0000313|EMBL:ACD83793.1};
OS   Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain
OS   V4)).
OC   Bacteria; Pseudomonadati; Verrucomicrobiota; Methylacidiphilae;
OC   Methylacidiphilales; Methylacidiphilaceae;
OC   Methylacidiphilum (ex Ratnadevi et al. 2023).
OX   NCBI_TaxID=481448 {ECO:0000313|EMBL:ABX56590.1};
RN   [1] {ECO:0000313|EMBL:ABX56590.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=V4 {ECO:0000313|EMBL:ABX56590.1};
RX   PubMed=18004300; DOI=10.1038/nature06411;
RA   Dunfield P.F., Yuryev A., Senin P., Smirnova A.V., Stott M.B., Hou S.,
RA   Ly B., Saw J.H., Zhou Z., Ren Y., Wang J., Mountain B.W., Crowe M.A.,
RA   Weatherby T.M., Bodelier P.L.E., Liesack W., Feng L., Wang L., Alam M.;
RT   "Methane oxidation by an extremely acidophilic bacterium of the phylum
RT   Verrucomicrobia.";
RL   Nature 450:879-882(2007).
RN   [2] {ECO:0000313|EMBL:ACD83793.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate V4, and V4 {ECO:0000313|EMBL:ACD83793.1};
RX   PubMed=18593465; DOI=10.1186/1745-6150-3-26;
RA   Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y.,
RA   Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V.,
RA   Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.,
RA   Feng L., Wang L., Alam M.;
RT   "Complete genome sequence of the extremely acidophilic methanotroph isolate
RT   V4, Methylacidiphilum infernorum, a representative of the bacterial phylum
RT   Verrucomicrobia.";
RL   Biol. Direct 3:26-26(2008).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = phosphoenolpyruvate +
CC         hydrogencarbonate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00048995, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; EU223848; ABX56590.1; -; Genomic_DNA.
DR   EMBL; CP000975; ACD83793.1; -; Genomic_DNA.
DR   RefSeq; WP_012464075.1; NC_010794.1.
DR   AlphaFoldDB; A9QPD2; -.
DR   STRING; 481448.Minf_1739; -.
DR   KEGG; min:Minf_1739; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_0; -.
DR   OMA; AAAIPQC; -.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_kinase-like_dom.
DR   NCBIfam; NF000584; ppc; 1.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595}; Coiled coil {ECO:0000256|SAM:Coils};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:ABX56590.1}.
FT   COILED          128..155
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   ACT_SITE        161
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        589
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   931 AA;  107056 MW;  7AA27B536340B1FD CRC64;
     MSVRKQLSSL QRISPEHDFS LLSRTIHFLG DTLGKVITQL EGEKILSIEE TIRKLAKESR
     KGKEEATRKL SDIVRGLDID MAYRMAMAFT CYFELVNLAE ENYRVRILMK RRINQLNHPQ
     KAIPKESIES AISQLKKAKV KKEEIQEILE KMEIVLVFTA HPTEIKRQTI LNKLCEISSY
     LKKESFNSKP SPAIHSTVER LIASLWLTER SRSKNPQVLD EVRTGLWYFE HTLWDVIPEL
     HNELQRVLDI YYPGVTLKPK WISFGSWIGG DRDGNFQVTT KTTATTFILQ RNLALRKVSQ
     SLERLSEIFS ISSQRIPPTK KVIRLLNEKL KSFPSLKEEY ERYPNEPYRL VLLALKKEVE
     NSINKISEND LLDFFHDCKP DTTLKAITEV LEALKENFDA PQTRIFLEGE FQKLAQCIEV
     FGIHIFSLDI RQHSAIHEEA IEEILRLNGH PEISYALLDE KEKVGLLNKL LEGPLPSLHS
     LYNRASDKLK EVLGPILVFS RSIAILGKEV CGCYLISMTS GLSDILEVLY LCKICRCSID
     IAPLFETLSD LKSSPQTLQD LLSHPLYRKY LQERSDKQIV MLGYSDSNKD CGYMTSNWWL
     FQIQEKLHEV CRVHGVSLVL FHGRGGTIAR GGGPAAKAIL AQPKGLLEGK IRITEQGEVL
     STRYHDFDIA FRILEQTTYG VLLAIHKSRK KTILPKSWEK TIECIAEESY RAYTHLLHQE
     PDFLRFWQEV TPINEIGSLK IASRPIFRFR LNNFEDLRAI PWVFSWMQTR FVIPGWYGIG
     SSLKKVIETS PKALSILQKM YEKWTFFQTV IDNAQLSLAK TDIDIARLYC DLASDKNLAQ
     RIFSIIEAEY KKTVETILKI TLQNNILDHE PILQRSIQLR NPYVDPLNYI QVEMIKRNRA
     GQPPTALEID KIKSVIELTI NGISAGLRNT G
//