ID MAO1_SHEB9 Reviewed; 562 AA. AC A9L2F4; DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot. DT 05-FEB-2008, sequence version 1. DT 04-NOV-2026, entry version 94. DE RecName: Full=NAD-dependent malic enzyme {ECO:0000255|HAMAP-Rule:MF_01619}; DE Short=NAD-ME {ECO:0000255|HAMAP-Rule:MF_01619}; DE EC=1.1.1.38 {ECO:0000255|HAMAP-Rule:MF_01619}; GN Name=maeA {ECO:0000255|HAMAP-Rule:MF_01619}; GN OrderedLocusNames=Sbal195_3757; OS Shewanella baltica (strain OS195). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; OC Alteromonadales; Shewanellaceae; Shewanella. OX NCBI_TaxID=399599; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=OS195; RG US DOE Joint Genome Institute; RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Brettar I., RA Rodrigues J., Konstantinidis K., Klappenbach J., Hofle M., Tiedje J., RA Richardson P.; RT "Complete sequence of chromosome of Shewanella baltica OS195."; RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=(S)-malate + NAD(+) = pyruvate + CO2 + NADH; CC Xref=Rhea:RHEA:12653, ChEBI:CHEBI:15361, ChEBI:CHEBI:15589, CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.38; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01619}; CC -!- CATALYTIC ACTIVITY: CC Reaction=oxaloacetate + H(+) = pyruvate + CO2; Xref=Rhea:RHEA:15641, CC ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, CC ChEBI:CHEBI:16526; EC=1.1.1.38; Evidence={ECO:0000255|HAMAP- CC Rule:MF_01619}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP- CC Rule:MF_01619}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP- CC Rule:MF_01619}; CC Note=Divalent metal cations. Prefers magnesium or manganese. CC {ECO:0000255|HAMAP-Rule:MF_01619}; CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01619}. CC -!- SIMILARITY: Belongs to the malic enzymes family. {ECO:0000255|HAMAP- CC Rule:MF_01619}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000891; ABX50917.1; -; Genomic_DNA. DR RefSeq; WP_006084095.1; NC_009997.1. DR AlphaFoldDB; A9L2F4; -. DR SMR; A9L2F4; -. DR GeneID; 11773776; -. DR KEGG; sbn:Sbal195_3757; -. DR HOGENOM; CLU_011405_5_2_6; -. DR OMA; AACERPM; -. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0004471; F:malate dehydrogenase (decarboxylating) (NAD+) activity; IEA:UniProtKB-UniRule. DR GO; GO:0051287; F:NAD binding; IEA:InterPro. DR GO; GO:0008948; F:oxaloacetate decarboxylase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006108; P:malate metabolic process; IEA:TreeGrafter. DR CDD; cd05312; NAD_bind_1_malic_enz; 1. DR FunFam; 3.40.50.10380:FF:000001; NAD-dependent malic enzyme; 1. DR FunFam; 3.40.50.720:FF:000055; NAD-dependent malic enzyme; 1. DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR HAMAP; MF_01619; NAD_malic_enz; 1. DR InterPro; IPR046346; Aminoacid_DH-like_N_sf. DR InterPro; IPR015884; Malic_enzyme_CS. DR InterPro; IPR012301; Malic_N_dom. DR InterPro; IPR037062; Malic_N_dom_sf. DR InterPro; IPR012302; Malic_NAD-bd. DR InterPro; IPR001891; Malic_OxRdtase. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR023667; NAD_malic_enz_proteobac. DR NCBIfam; NF010052; maeA; 1. DR PANTHER; PTHR23406; MALIC ENZYME-RELATED; 1. DR PANTHER; PTHR23406:SF34; NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL; 1. DR Pfam; PF00390; malic; 1. DR Pfam; PF03949; Malic_M; 1. DR PIRSF; PIRSF000106; ME; 1. DR PRINTS; PR00072; MALOXRDTASE. DR SMART; SM01274; malic; 1. DR SMART; SM00919; Malic_M; 1. DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. DR PROSITE; PS00331; MALIC_ENZYMES; 1. PE 3: Inferred from homology; KW Metal-binding; NAD; Oxidoreductase. FT CHAIN 1..562 FT /note="NAD-dependent malic enzyme" FT /id="PRO_1000088074" FT ACT_SITE 101 FT /note="Proton donor" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT ACT_SITE 172 FT /note="Proton acceptor" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT BINDING 154 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT BINDING 243 FT /ligand="a divalent metal cation" FT /ligand_id="ChEBI:CHEBI:60240" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT BINDING 244 FT /ligand="a divalent metal cation" FT /ligand_id="ChEBI:CHEBI:60240" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT BINDING 267 FT /ligand="a divalent metal cation" FT /ligand_id="ChEBI:CHEBI:60240" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT BINDING 267 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT BINDING 415 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" FT SITE 267 FT /note="Important for activity" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01619" SQ SEQUENCE 562 AA; 62102 MW; D15A8F80DF361D67 CRC64; MDDNKRPLYL PFAGPAILEA PLINKGSAFS EEERIFFNLE GLVPYAIETI EEQASRAYDQ FRSFNNDLDK HIYLRNIQDT NETLFYRLVQ NNISEMMPII YTPTVGLACE RFSKNYRRNR GLFISYPNKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG GISPAYTLPI TLDVGTDNPQ LLEDPMYMGW RHPRIGGEEY AEFIEAFMQA VHVRWPDTLI QFEDFAQKNA MPILERYKDR YCCFNDDIQG TAAVAVGSLL AACKAAGTEL NQQRIAFLGA GSAGCGIAEA IVAQMVSEGI SDEQARTQVC MVDRWGLLLD NMPNLLPFQQ KLAQKCTNIQ NWSNFSDNIS LLDVVNNAKP TVLIGVSGVP GLFTEEIIRA MHSHCARPII FPLSNPTSRV EATPKDILHW TSGQALVATG SPFEPVVVDG ETYEIAQCNN SFIFPGIGLG VLASGARHVS DAMLMASSRA LAECSPLAID GSGPLLPKLE DIHAVSKHIA FAVGKVAVEQ GLTLPMSDEI LQQSIEGNFW SPEYRRYKRT SF //