ID   A9KTC1_LACP7            Unreviewed;       514 AA.
AC   A9KTC1;
DT   05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   05-FEB-2008, sequence version 1.
DT   10-JUN-2026, entry version 74.
DE   SubName: Full=Glycoside hydrolase family 43 {ECO:0000313|EMBL:ABX43751.1};
GN   OrderedLocusNames=Cphy_3398 {ECO:0000313|EMBL:ABX43751.1};
OS   Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
OS   (Clostridium phytofermentans).
OC   Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
OC   Lachnospiraceae.
OX   NCBI_TaxID=357809 {ECO:0000313|EMBL:ABX43751.1, ECO:0000313|Proteomes:UP000000370};
RN   [1] {ECO:0000313|Proteomes:UP000000370}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700394 / DSM 18823 / ISDg
RC   {ECO:0000313|Proteomes:UP000000370};
RA   Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L.,
RA   LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T.,
RA   Bruce D., Detter J.C., Han C., Kuske C., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E.A., Richardson P.;
RT   "Complete genome sequence of Clostridium phytofermentans ISDg.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR   EMBL; CP000885; ABX43751.1; -; Genomic_DNA.
DR   RefSeq; WP_012201400.1; NC_010001.1.
DR   AlphaFoldDB; A9KTC1; -.
DR   STRING; 357809.Cphy_3398; -.
DR   KEGG; cpy:Cphy_3398; -.
DR   eggNOG; COG3507; Bacteria.
DR   HOGENOM; CLU_016508_2_1_9; -.
DR   OrthoDB; 9801455at2; -.
DR   Proteomes; UP000000370; Chromosome.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd09000; GH43_SXA-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   Gene3D; 2.115.10.20; Glycosyl hydrolase domain, family 43; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   InterPro; IPR051795; Glycosyl_Hydrlase_43.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000370}.
FT   DOMAIN          316..513
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        13
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        178
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            126
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   514 AA;  58745 MW;  B68D31F8938ED5A5 CRC64;
     MIQNPILRGF CPDPSIIRVG EDYYIATSTF EWWPGIHLFH SKDLKNWEQI PSPIARRSQV
     DMIGNPTSGG IWAPCLSYSD GIYYIVFTDV KTKKGRFYNN HNYLIQSNAI DGEWSEPIYL
     NSTGFDPSLF HDTDGRKYLV NMRNGFKGIL LQEYDTKKKT LVGEIKNIFK GTSYGYTEGP
     HLYHIGDWYY LMTAEGGTGY GHCVTVARSK DIWGPYEVDP SNPMLTSKED DDSILKKCGH
     ADFTDTGNGE WYMVHLCSRP REGTKDSLLG RETAIQKVEW TEDGWLRLNN QTGKADVEVA
     EPIGITSYPV PKRENRDDFD NNTLDIVYSS PRIPLGEKAS LSERAGYLRL YGQESLNSLH
     SVSLLARRQT EYYAKAMTGL EFKPSHPEQV AGITYFYDAL NHFIFGKSVD EEGREVLILL
     RNDTGVYEDL IEPLVLEEHN QLHLQVETDE YGAYAYFSYS YDGLSWFKIT GEYSTKILTD
     EHCRGFTGAH FGIFCFDMTG LSLPADFDYF HYEE
//