ID   A9KMY2_LACP7            Unreviewed;       543 AA.
AC   A9KMY2;
DT   05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   05-FEB-2008, sequence version 1.
DT   10-JUN-2026, entry version 70.
DE   SubName: Full=Glycoside hydrolase family 43 {ECO:0000313|EMBL:ABX42993.1};
GN   OrderedLocusNames=Cphy_2632 {ECO:0000313|EMBL:ABX42993.1};
OS   Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
OS   (Clostridium phytofermentans).
OC   Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
OC   Lachnospiraceae.
OX   NCBI_TaxID=357809 {ECO:0000313|EMBL:ABX42993.1, ECO:0000313|Proteomes:UP000000370};
RN   [1] {ECO:0000313|Proteomes:UP000000370}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700394 / DSM 18823 / ISDg
RC   {ECO:0000313|Proteomes:UP000000370};
RA   Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L.,
RA   LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T.,
RA   Bruce D., Detter J.C., Han C., Kuske C., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E.A., Richardson P.;
RT   "Complete genome sequence of Clostridium phytofermentans ISDg.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR   EMBL; CP000885; ABX42993.1; -; Genomic_DNA.
DR   RefSeq; WP_012200645.1; NC_010001.1.
DR   AlphaFoldDB; A9KMY2; -.
DR   STRING; 357809.Cphy_2632; -.
DR   KEGG; cpy:Cphy_2632; -.
DR   eggNOG; COG3507; Bacteria.
DR   HOGENOM; CLU_016508_1_0_9; -.
DR   OrthoDB; 9801455at2; -.
DR   Proteomes; UP000000370; Chromosome.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd09001; GH43_FsAxh1-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   Gene3D; 2.115.10.20; Glycosyl hydrolase domain, family 43; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   InterPro; IPR051795; Glycosyl_Hydrlase_43.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000370}.
FT   DOMAIN          325..543
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        22
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        188
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            129
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   543 AA;  61445 MW;  025B2A405A401D0F CRC64;
     MWIADLENGN YRNPLLYADY SDPDAIRVGD DYFMVASSFC NAPALPLLHS KDLVNWKVVN
     YVLDKIPEFR YRNPMHGCGV WAPSIRYHEG TYYVCFPMPD EGIYMTTTKD PFGKWSEPVN
     IRPGAGWIDP CPFWDEDGKA YLVAGVAKSR IGYKSVLHMV EMQPDGMGLI GEDVRIFDGD
     TNGNVTTEGP KMHKKNGWYY VFAPAGGVKT GWQLVLRSKN VFGPYEHKVV MKQGDTLVNG
     PHQGAWVDTV TGEDWFLHFQ DVYAAGRIVH LQPMHWENEW PIIGVNKEGN DYGEPVMEYK
     KPNVGKTEEE LQNNPLYPVC EPDTTDEFEG DSLGLQWQWN ANPDIDWYEL GNSKIKLNAV
     PSVPQRPISD YRNLLLQKWP APEFSCITKM SFAGLQDGDT AGIVSLGMEY SAMAVSKKNG
     SYAIEKITGI QNFDCDVSYA DQNKVSISLT DSDFKDREKT VFLKYEVKCV GYVDNMEMDI
     QVKHVPQETI TMSYSLDGEE FKEAFTINAK AGRWVGVKNG VFCNHDNTMN GGGYVEVDYV
     RYQ
//