ID   A8FG03_BACP2            Unreviewed;       492 AA.
AC   A8FG03;
DT   13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2007, sequence version 1.
DT   02-SEP-2026, entry version 81.
DE   RecName: Full=non-reducing end alpha-L-arabinofuranosidase {ECO:0000256|ARBA:ARBA00012670};
DE            EC=3.2.1.55 {ECO:0000256|ARBA:ARBA00012670};
GN   OrderedLocusNames=BPUM_2508 {ECO:0000313|EMBL:ABV63170.1};
OS   Bacillus pumilus (strain SAFR-032).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Bacillaceae;
OC   Bacillus.
OX   NCBI_TaxID=315750 {ECO:0000313|EMBL:ABV63170.1, ECO:0000313|Proteomes:UP000001355};
RN   [1] {ECO:0000313|EMBL:ABV63170.1, ECO:0000313|Proteomes:UP000001355}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032 {ECO:0000313|EMBL:ABV63170.1,
RC   ECO:0000313|Proteomes:UP000001355};
RX   PubMed=17895969; DOI=10.1371/journal.pone.0000928;
RA   Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D.,
RA   Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H.,
RA   Lee S., Nazareth L., Blyth P., Holder M., Buhay C., Tirumalai M.R., Liu Y.,
RA   Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P.,
RA   Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D.,
RA   Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F.,
RA   Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K.,
RA   Weinstock G.M.;
RT   "Paradoxical DNA repair and peroxide resistance gene conservation in
RT   Bacillus pumilus SAFR-032.";
RL   PLoS ONE 2:E928-E928(2007).
RN   [2] {ECO:0000313|EMBL:ABV63170.1, ECO:0000313|Proteomes:UP000001355}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032 {ECO:0000313|EMBL:ABV63170.1,
RC   ECO:0000313|Proteomes:UP000001355};
RX   PubMed=23812891; DOI=10.1007/s00792-013-0559-z;
RA   Tirumalai M.R., Fox G.E.;
RT   "An ICEBs1-like element may be associated with the extreme radiation and
RT   desiccation resistance of Bacillus pumilus SAFR-032 spores.";
RL   Extremophiles 17:767-774(2013).
RN   [3] {ECO:0000313|EMBL:ABV63170.1, ECO:0000313|Proteomes:UP000001355}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032 {ECO:0000313|EMBL:ABV63170.1,
RC   ECO:0000313|Proteomes:UP000001355};
RX   PubMed=23799069;
RA   Tirumalai M.R., Rastogi R., Zamani N., O'Bryant Williams E., Allen S.,
RA   Diouf F., Kwende S., Weinstock G.M., Venkateswaran K.J., Fox G.E.;
RT   "Candidate genes that may be responsible for the unusual resistances
RT   exhibited by Bacillus pumilus SAFR-032 spores.";
RL   PLoS ONE 8:E66012-E66012(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside
CC         residues in alpha-L-arabinosides.; EC=3.2.1.55;
CC         Evidence={ECO:0000256|ARBA:ARBA00001462};
CC   -!- PATHWAY: Glycan metabolism. {ECO:0000256|ARBA:ARBA00004881}.
CC   -!- SUBUNIT: Homohexamer; trimer of dimers.
CC       {ECO:0000256|ARBA:ARBA00011165}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 51 family.
CC       {ECO:0000256|ARBA:ARBA00007186}.
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DR   EMBL; CP000813; ABV63170.1; -; Genomic_DNA.
DR   RefSeq; WP_012010821.1; NC_009848.4.
DR   AlphaFoldDB; A8FG03; -.
DR   STRING; 315750.BPUM_2508; -.
DR   PaxDb; 315750-BPUM_2508; -.
DR   GeneID; 5621773; -.
DR   KEGG; bpu:BPUM_2508; -.
DR   eggNOG; COG3534; Bacteria.
DR   HOGENOM; CLU_017810_2_0_9; -.
DR   OrthoDB; 9758333at2; -.
DR   Proteomes; UP000001355; Chromosome.
DR   GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046373; P:L-arabinose metabolic process; IEA:InterPro.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:TreeGrafter.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR010720; Alpha-L-AF_C.
DR   InterPro; IPR055235; ASD1_cat.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   PANTHER; PTHR43576; ALPHA-L-ARABINOFURANOSIDASE C-RELATED; 1.
DR   PANTHER; PTHR43576:SF2; INTRACELLULAR EXO-ALPHA-L-ARABINOFURANOSIDASE 2; 1.
DR   Pfam; PF06964; Alpha-L-AF_C; 1.
DR   Pfam; PF22848; ASD1_dom; 1.
DR   SMART; SM00813; Alpha-L-AF_C; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001355}.
FT   DOMAIN          293..483
FT                   /note="Alpha-L-arabinofuranosidase C-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00813"
SQ   SEQUENCE   492 AA;  56152 MW;  FF82692174F66AA0 CRC64;
     MKGTVKVNDV IGRISKHIYG HFQEHLGRGI YDGIWVGKDS EIDHIEGIRT DVLKALQALH
     IPVLRWPGGC FADEYHWANG VGDLSERKPM VNTHWGGTVE SNEFGTHEFM KLCELLECEP
     YICGNVGSGT VQELADWVEY ITFPKGTPMS DWRIQNGKHE PWDLTYVGVG NESWGCGGNM
     TPEYYADLYK RYQTYVREFV GQSIYKIACG ANSNDVNWTK VLMERAAPWM DGLSLHYYTV
     PGTWEKKGSA TEFDEDEWFI TLKKAYEMER LVLDHGEIMD RYDPDKRIGM IIDEWGTWYD
     PEPGTNPGFL YQQNTLRDAL VCALHFHIFH RHCQRIHMAN IAQTVNVLQA MVLTEDEKML
     LTPTYHVFHM FQVHQVEEAL EVDFVSTPYE RSGEKIPQVS VSASRSSDKM HISFCHLNPH
     EQNEVSLAIE GLDAQTKVTG RVLTADRMNA HNTFDDPHAV QPVEFQQFAV KSGELTIDLP
     PMSVVMITID LA
//