ID   A8FDV5_BACP2            Unreviewed;       446 AA.
AC   A8FDV5;
DT   13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2007, sequence version 1.
DT   02-SEP-2026, entry version 88.
DE   SubName: Full=Alpha-galactosidase {ECO:0000313|EMBL:ABV62422.1};
GN   OrderedLocusNames=BPUM_1750 {ECO:0000313|EMBL:ABV62422.1};
OS   Bacillus pumilus (strain SAFR-032).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Bacillaceae;
OC   Bacillus.
OX   NCBI_TaxID=315750 {ECO:0000313|EMBL:ABV62422.1, ECO:0000313|Proteomes:UP000001355};
RN   [1] {ECO:0000313|EMBL:ABV62422.1, ECO:0000313|Proteomes:UP000001355}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032 {ECO:0000313|EMBL:ABV62422.1,
RC   ECO:0000313|Proteomes:UP000001355};
RX   PubMed=17895969; DOI=10.1371/journal.pone.0000928;
RA   Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D.,
RA   Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H.,
RA   Lee S., Nazareth L., Blyth P., Holder M., Buhay C., Tirumalai M.R., Liu Y.,
RA   Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P.,
RA   Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D.,
RA   Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F.,
RA   Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K.,
RA   Weinstock G.M.;
RT   "Paradoxical DNA repair and peroxide resistance gene conservation in
RT   Bacillus pumilus SAFR-032.";
RL   PLoS ONE 2:E928-E928(2007).
RN   [2] {ECO:0000313|EMBL:ABV62422.1, ECO:0000313|Proteomes:UP000001355}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032 {ECO:0000313|EMBL:ABV62422.1,
RC   ECO:0000313|Proteomes:UP000001355};
RX   PubMed=23812891; DOI=10.1007/s00792-013-0559-z;
RA   Tirumalai M.R., Fox G.E.;
RT   "An ICEBs1-like element may be associated with the extreme radiation and
RT   desiccation resistance of Bacillus pumilus SAFR-032 spores.";
RL   Extremophiles 17:767-774(2013).
RN   [3] {ECO:0000313|EMBL:ABV62422.1, ECO:0000313|Proteomes:UP000001355}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032 {ECO:0000313|EMBL:ABV62422.1,
RC   ECO:0000313|Proteomes:UP000001355};
RX   PubMed=23799069;
RA   Tirumalai M.R., Rastogi R., Zamani N., O'Bryant Williams E., Allen S.,
RA   Diouf F., Kwende S., Weinstock G.M., Venkateswaran K.J., Fox G.E.;
RT   "Candidate genes that may be responsible for the unusual resistances
RT   exhibited by Bacillus pumilus SAFR-032 spores.";
RL   PLoS ONE 8:E66012-E66012(2013).
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC         Evidence={ECO:0000256|RuleBase:RU361152};
CC       Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC       {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
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DR   EMBL; CP000813; ABV62422.1; -; Genomic_DNA.
DR   RefSeq; WP_012010149.1; NC_009848.4.
DR   AlphaFoldDB; A8FDV5; -.
DR   STRING; 315750.BPUM_1750; -.
DR   PaxDb; 315750-BPUM_1750; -.
DR   GeneID; 5621011; -.
DR   KEGG; bpu:BPUM_1750; -.
DR   eggNOG; COG1486; Bacteria.
DR   HOGENOM; CLU_045951_1_1_9; -.
DR   OrthoDB; 9808275at2; -.
DR   Proteomes; UP000001355; Chromosome.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd05297; GH4_alpha_glucosidase_galactosidase; 1.
DR   Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR   InterPro; IPR053715; GH4_Enzyme_sf.
DR   InterPro; IPR001088; Glyco_hydro_4.
DR   InterPro; IPR022616; Glyco_hydro_4_C.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   NCBIfam; NF011657; PRK15076.1; 1.
DR   PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR32092:SF6; ALPHA-GALACTOSIDASE; 1.
DR   Pfam; PF02056; Glyco_hydro_4; 1.
DR   Pfam; PF11975; Glyco_hydro_4C; 1.
DR   PRINTS; PR00732; GLHYDRLASE4.
DR   SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW   Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|PIRSR:PIRSR601088-3};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR601088-3};
KW   NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU361152};
KW   Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001355}.
FT   DOMAIN          194..405
FT                   /note="Glycosyl hydrolase family 4 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF11975"
FT   BINDING         148
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT   BINDING         169
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT   BINDING         199
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT   SITE            110
FT                   /note="Increases basicity of active site Tyr"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ   SEQUENCE   446 AA;  50584 MW;  9B8F4AF126EB4136 CRC64;
     MSKITFIGAG STVFAKNILG DCLFVPALSD FEFALYDIDH KRLKESETML RHLKENYGAH
     VTITSYHNRK EALKDAKYVI NAIQVGGYRP STVIDFEIPK KYGLRQTIAD TVGIGGIFRF
     LRTFPVMLDL AKEMEEVCPN ALLLNYTNPM ATLTGAMLRY TPVQTVGLCH SVQVCTKDLF
     ESLEMDHEGI EEKIAGINHM AWLLEVKRDG KDLYPDIKRR AKEKQKSRHH DMVRFELMDK
     FGYYVTESSE HNAEYHPYFI KSRYPELIGQ FNIPLDEYPR RCEEQINNWN SMKHDLVSNT
     QITHTRSKEY GSRIIEAIET NVPFKFAGNV LNTGGLIHNL PEKACVEVPC VADRSGIMPC
     HVGEIPEQLA GLNRTNISSQ LMTIEAAISG KKEHVYQAAL LDPHTSAELS IDDIIKLCDE
     LIEAHSEMLP HFADPNQYAA STNLQK
//