ID   RLMF_CITK8              Reviewed;         308 AA.
AC   A8AIX5;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   18-JUN-2025, entry version 84.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase F {ECO:0000255|HAMAP-Rule:MF_01848};
DE            EC=2.1.1.181 {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=23S rRNA mA1618 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=rRNA adenine N-6-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
GN   Name=rlmF {ECO:0000255|HAMAP-Rule:MF_01848};
GN   OrderedLocusNames=CKO_02316;
OS   Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=290338;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-895 / CDC 4225-83 / SGSC4696;
RG   The Citrobacter koseri Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA   Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA   Johnson M., Thiruvilangam P., Wilson R.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Specifically methylates the adenine in position 1618 of 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(1618) in 23S rRNA + S-adenosyl-L-methionine = N(6)-
CC         methyladenosine(1618) in 23S rRNA + S-adenosyl-L-homocysteine + H(+);
CC         Xref=Rhea:RHEA:16497, Rhea:RHEA-COMP:10229, Rhea:RHEA-COMP:10231,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.181;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01848};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC       family. {ECO:0000255|HAMAP-Rule:MF_01848}.
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DR   EMBL; CP000822; ABV13438.1; -; Genomic_DNA.
DR   RefSeq; WP_012133165.1; NC_009792.1.
DR   AlphaFoldDB; A8AIX5; -.
DR   SMR; A8AIX5; -.
DR   STRING; 290338.CKO_02316; -.
DR   GeneID; 45136224; -.
DR   KEGG; cko:CKO_02316; -.
DR   HOGENOM; CLU_027534_3_0_6; -.
DR   OrthoDB; 1115728at2; -.
DR   Proteomes; UP000008148; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070475; P:rRNA base methylation; IEA:TreeGrafter.
DR   FunFam; 3.40.50.150:FF:000045; Ribosomal RNA large subunit methyltransferase F; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   HAMAP; MF_01848; 23SrRNA_methyltr_F; 1.
DR   InterPro; IPR010286; METTL16/RlmF.
DR   InterPro; IPR016909; rRNA_lsu_MeTfrase_F.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; NF008725; PRK11727.1; 1.
DR   PANTHER; PTHR13393:SF0; RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16; 1.
DR   PANTHER; PTHR13393; SAM-DEPENDENT METHYLTRANSFERASE; 1.
DR   Pfam; PF05971; Methyltransf_10; 1.
DR   PIRSF; PIRSF029038; Mtase_YbiN_prd; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..308
FT                   /note="Ribosomal RNA large subunit methyltransferase F"
FT                   /id="PRO_0000349898"
FT   REGION          190..212
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   308 AA;  34254 MW;  E5886F591FAE0B47 CRC64;
     MSAQKPGLHP RNRHHHRYDL ATLCQVTPEL AQFLTRTPAG EQSVDFANPL AVKALNKALL
     AYFYQVANWD IPEGFLCPPV PGRADYIHHL ADLLGETTGA IPADASVLDV GTGANCIYPL
     IGVHEYGWRF TGSEVHAQAL ASAQVIISGN PGLTRSIRLR RQKDPAAIFN GIIHKNEQYD
     ATLCNPPFHD SAASARAGSE RKRRNLGQDK NDALNFGGQQ QELWCEGGEV AFIKTMIAES
     QAFGRQVMWF TTLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMDDDQR
     RRFMARKR
//