ID   A7RHV6_NEMVE            Unreviewed;       427 AA.
AC   A7RHV6;
DT   02-OCT-2007, integrated into UniProtKB/TrEMBL.
DT   02-OCT-2007, sequence version 1.
DT   02-SEP-2026, entry version 80.
DE   RecName: Full=Phosphatidylinositol N-acetylglucosaminyltransferase subunit A {ECO:0000256|ARBA:ARBA00068191};
DE            EC=2.4.1.198 {ECO:0000256|ARBA:ARBA00012420};
DE   AltName: Full=GlcNAc-PI synthesis protein {ECO:0000256|ARBA:ARBA00032160};
DE   AltName: Full=Phosphatidylinositol-glycan biosynthesis class A protein {ECO:0000256|ARBA:ARBA00083199};
DE   Flags: Fragment;
GN   ORFNames=NEMVEDRAFT_v1g158845 {ECO:0000313|EMBL:EDO49044.1};
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351 {ECO:0000313|EMBL:EDO49044.1, ECO:0000313|Proteomes:UP000001593};
RN   [1] {ECO:0000313|EMBL:EDO49044.1, ECO:0000313|Proteomes:UP000001593}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CH2 X CH6 {ECO:0000313|Proteomes:UP000001593};
RX   PubMed=17615350; DOI=10.1126/science.1139158;
RA   Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA   Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA   Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA   Technau U., Martindale M.Q., Rokhsar D.S.;
RT   "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT   genomic organization.";
RL   Science 317:86-94(2007).
CC   -!- FUNCTION: Catalytic subunit of the glycosylphosphatidylinositol-N-
CC       acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the
CC       transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to
CC       phosphatidylinositol and participates in the first step of GPI
CC       biosynthesis. {ECO:0000256|ARBA:ARBA00058926}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + UDP-N-
CC         acetyl-alpha-D-glucosamine = a 6-(N-acetyl-alpha-D-glucosaminyl)-1-
CC         (1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol + UDP + H(+);
CC         Xref=Rhea:RHEA:14789, ChEBI:CHEBI:15378, ChEBI:CHEBI:57265,
CC         ChEBI:CHEBI:57705, ChEBI:CHEBI:57880, ChEBI:CHEBI:58223;
CC         EC=2.4.1.198; Evidence={ECO:0000256|ARBA:ARBA00093192};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14790;
CC         Evidence={ECO:0000256|ARBA:ARBA00093192};
CC   -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC       biosynthesis. {ECO:0000256|ARBA:ARBA00004687}.
CC   -!- SUBCELLULAR LOCATION: Rough endoplasmic reticulum membrane
CC       {ECO:0000256|ARBA:ARBA00094543}; Single-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00094543}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DS469511; EDO49044.1; -; Genomic_DNA.
DR   AlphaFoldDB; A7RHV6; -.
DR   STRING; 45351.A7RHV6; -.
DR   KEGG; nve:5521300; -.
DR   eggNOG; KOG1111; Eukaryota.
DR   HOGENOM; CLU_009583_19_0_1; -.
DR   InParanoid; A7RHV6; -.
DR   OMA; SHFWMSG; -.
DR   OrthoDB; 734129at2759; -.
DR   PhylomeDB; A7RHV6; -.
DR   UniPathway; UPA00196; -.
DR   Proteomes; UP000001593; Unassembled WGS sequence.
DR   GO; GO:0000506; C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; IBA:GO_Central.
DR   GO; GO:0030867; C:rough endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0017176; F:phosphatidylinositol N-acetylglucosaminyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR   CDD; cd03796; GT4_PIG-A-like; 1.
DR   FunFam; 3.40.50.2000:FF:000059; Phosphatidylinositol glycan anchor biosynthesis class A; 1.
DR   FunFam; 3.40.50.2000:FF:000026; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; 1.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR   InterPro; IPR039507; PIG-A/GPI3.
DR   InterPro; IPR013234; PIGA_GPI_anchor_biosynthesis.
DR   PANTHER; PTHR45871; N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN; 1.
DR   PANTHER; PTHR45871:SF1; PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A; 1.
DR   Pfam; PF13692; Glyco_trans_1_4; 1.
DR   Pfam; PF08288; PIGA; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   4: Predicted;
KW   Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW   Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW   GPI-anchor biosynthesis {ECO:0000256|ARBA:ARBA00022502};
KW   Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001593};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        400..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          45..134
FT                   /note="PIGA GPI anchor biosynthesis"
FT                   /evidence="ECO:0000259|Pfam:PF08288"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:EDO49044.1"
SQ   SEQUENCE   427 AA;  48219 MW;  4C08AF4FBC9400A8 CRC64;
     MADRKHRICM VSDFFYPNMG GVESHIYQLS QRLIQRGHKV IVVTHSYGGR KGVRYLTNYL
     KVYYLPFGVF HNQCILPTLY LDLPILRYIF LRERITIVHG HSAFSTLCHD ALLHARTMGM
     KTIFTDHSLF GFADASSIIT NKFLQFSLAD VEHVICVSHT SKENTVLRAS TQSGMKPYIV
     SVIPNAVDAS MFTPCPSKRR KNCITVVVVS RLVYRKGMDL LAGIIPKLCH RHSDVNFLIG
     GDGPKRVVLE EVREQNQLHD RVELLGTLEH DKVRDVLVQG DVFLNTSLTE AFCMAIVEAA
     SCGLQVVSTR VGGVPEVLPP DMITMAEPSV QALVDALEDC IRRVRSRSVI DTHTAHERIR
     AMYTWTNVSK RTEKVYDTVA SLPSVPFEER LKRLYMCGSI AGKIFCVLAV IDLIILMMLE
     WLVPRKV
//