ID   A7NGF2_ROSCS            Unreviewed;       476 AA.
AC   A7NGF2;
DT   02-OCT-2007, integrated into UniProtKB/TrEMBL.
DT   02-OCT-2007, sequence version 1.
DT   02-SEP-2026, entry version 74.
DE   RecName: Full=site-specific DNA-methyltransferase (cytosine-N(4)-specific) {ECO:0000256|ARBA:ARBA00012185};
DE            EC=2.1.1.113 {ECO:0000256|ARBA:ARBA00012185};
GN   OrderedLocusNames=Rcas_0407 {ECO:0000313|EMBL:ABU56539.1};
OS   Roseiflexus castenholzii (strain DSM 13941 / HLO8).
OC   Bacteria; Bacillati; Chloroflexota; Chloroflexia; Chloroflexales;
OC   Roseiflexineae; Roseiflexaceae; Roseiflexus.
OX   NCBI_TaxID=383372 {ECO:0000313|EMBL:ABU56539.1, ECO:0000313|Proteomes:UP000000263};
RN   [1] {ECO:0000313|EMBL:ABU56539.1, ECO:0000313|Proteomes:UP000000263}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 13941 / HLO8 {ECO:0000313|Proteomes:UP000000263};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P.;
RT   "Complete sequence of Roseiflexus castenholzii DSM 13941.";
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC         methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+);
CC         Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC         Evidence={ECO:0000256|ARBA:ARBA00049120};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC       subfamily. {ECO:0000256|ARBA:ARBA00010203}.
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DR   EMBL; CP000804; ABU56539.1; -; Genomic_DNA.
DR   RefSeq; WP_011997943.1; NC_009767.1.
DR   AlphaFoldDB; A7NGF2; -.
DR   STRING; 383372.Rcas_0407; -.
DR   REBASE; 16025; M.Rca13941ORF407P.
DR   KEGG; rca:Rcas_0407; -.
DR   eggNOG; COG0863; Bacteria.
DR   HOGENOM; CLU_027633_0_0_0; -.
DR   OrthoDB; 9800801at2; -.
DR   Proteomes; UP000000263; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR   InterPro; IPR017985; MeTrfase_CN4_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR   PROSITE; PS00093; N4_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:ABU56539.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000263};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ABU56539.1}.
SQ   SEQUENCE   476 AA;  53714 MW;  218B033608381BFA CRC64;
     MLNPAKVSDI LKGEQNIGLS KIAHLDKQLF QRFKSKFAVQ PSLSRLLVSF QANKTRPVYR
     WYKFKEAFSA SLVEHLFHKY GITAGRILDP FAGSGTALFA ASAMGIDADG IELLPIGHEI
     ITAKRILDAE FTSEDFERLR RWSELRVWEQ SETRVPLPEL RITQGAYPGK TKEAIEKYIG
     ACQQENSRVQ AVLRFALLCV LESISFTRKD GQYLRWDYRS GRTHGKKIFD KGEIPEFGQA
     ISEKLNEILE DASPVHQTTL FPIEKLQGQI CLYDGSCLQV LPRLSDNAYD AIMTSPPYCN
     RYDYTRTYAL ELALLGTGEQ GLLRLRQEML SCTVENRAKD LLSINPLWTT ALAAADEQDL
     LQAILTYLED QKAQRALNNN GIPRMVGGYF YEMACVIAEC ARVLKPNAPL FMVNDNVRYA
     GASISVDMIL SDIAEKLGFQ VDHILVLPNG KGNSSQQMGE HGREPLRKCI YVWRKS
//