ID   A7MH18_CROS8            Unreviewed;       253 AA.
AC   A7MH18;
DT   02-OCT-2007, integrated into UniProtKB/TrEMBL.
DT   02-OCT-2007, sequence version 1.
DT   18-JUN-2025, entry version 105.
DE   RecName: Full=Uracil-DNA glycosylase {ECO:0000256|ARBA:ARBA00018429, ECO:0000256|HAMAP-Rule:MF_00148};
DE            Short=UDG {ECO:0000256|HAMAP-Rule:MF_00148};
DE            EC=3.2.2.27 {ECO:0000256|ARBA:ARBA00012030, ECO:0000256|HAMAP-Rule:MF_00148};
GN   Name=ung {ECO:0000256|HAMAP-Rule:MF_00148};
GN   OrderedLocusNames=ESA_00680 {ECO:0000313|EMBL:ABU75959.1};
OS   Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Cronobacter.
OX   NCBI_TaxID=290339 {ECO:0000313|EMBL:ABU75959.1, ECO:0000313|Proteomes:UP000000260};
RN   [1] {ECO:0000313|EMBL:ABU75959.1, ECO:0000313|Proteomes:UP000000260}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-894 {ECO:0000313|EMBL:ABU75959.1,
RC   ECO:0000313|Proteomes:UP000000260};
RX   PubMed=20221447; DOI=10.1371/journal.pone.0009556;
RA   Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L.,
RA   Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A.,
RA   Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K.,
RA   McClelland M., Forsythe S.J.;
RT   "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic
RT   hybridization analysis with other Cronobacter species.";
RL   PLoS ONE 5:E9556-E9556(2010).
CC   -!- FUNCTION: Excises uracil residues from the DNA which can arise as a
CC       result of misincorporation of dUMP residues by DNA polymerase or due to
CC       deamination of cytosine. {ECO:0000256|ARBA:ARBA00002631,
CC       ECO:0000256|HAMAP-Rule:MF_00148, ECO:0000256|RuleBase:RU003780}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes single-stranded DNA or mismatched double-stranded
CC         DNA and polynucleotides, releasing free uracil.; EC=3.2.2.27;
CC         Evidence={ECO:0000256|ARBA:ARBA00001400, ECO:0000256|HAMAP-
CC         Rule:MF_00148, ECO:0000256|RuleBase:RU003780};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00148}.
CC   -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC       UNG family. {ECO:0000256|ARBA:ARBA00008184, ECO:0000256|HAMAP-
CC       Rule:MF_00148, ECO:0000256|RuleBase:RU003780}.
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DR   EMBL; CP000783; ABU75959.1; -; Genomic_DNA.
DR   AlphaFoldDB; A7MH18; -.
DR   KEGG; esa:ESA_00680; -.
DR   HOGENOM; CLU_032162_3_1_6; -.
DR   Proteomes; UP000000260; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004844; F:uracil DNA N-glycosylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0097510; P:base-excision repair, AP site formation via deaminated base removal; IEA:TreeGrafter.
DR   CDD; cd10027; UDG-F1-like; 1.
DR   FunFam; 3.40.470.10:FF:000001; Uracil-DNA glycosylase; 1.
DR   Gene3D; 3.40.470.10; Uracil-DNA glycosylase-like domain; 1.
DR   HAMAP; MF_00148; UDG; 1.
DR   InterPro; IPR002043; UDG_fam1.
DR   InterPro; IPR018085; Ura-DNA_Glyclase_AS.
DR   InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR   InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR   NCBIfam; NF003588; PRK05254.1-1; 1.
DR   NCBIfam; NF003589; PRK05254.1-2; 1.
DR   NCBIfam; NF003591; PRK05254.1-4; 1.
DR   NCBIfam; NF003592; PRK05254.1-5; 1.
DR   NCBIfam; TIGR00628; ung; 1.
DR   PANTHER; PTHR11264; URACIL-DNA GLYCOSYLASE; 1.
DR   PANTHER; PTHR11264:SF0; URACIL-DNA GLYCOSYLASE; 1.
DR   Pfam; PF03167; UDG; 1.
DR   SMART; SM00986; UDG; 1.
DR   SMART; SM00987; UreE_C; 1.
DR   SUPFAM; SSF52141; Uracil-DNA glycosylase-like; 1.
DR   PROSITE; PS00130; U_DNA_GLYCOSYLASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00148};
KW   DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW   Rule:MF_00148};
KW   DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW   Rule:MF_00148};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00148};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000260}.
FT   DOMAIN          73..234
FT                   /note="Uracil-DNA glycosylase-like"
FT                   /evidence="ECO:0000259|SMART:SM00986"
FT   ACT_SITE        88
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00148,
FT                   ECO:0000256|PROSITE-ProRule:PRU10072"
SQ   SEQUENCE   253 AA;  28223 MW;  DCBBB1FD5635B81F CRC64;
     MDVPVLFFGN KALSLGNRKS QESEMTDKLT WHDVLADEKE KPYFKKILTT VAAEREAGVT
     IYPPQKEVFN AFRFTELGDV KVVILGQDPY HGPGQAHGLA FSVRPGVAPP PSLVNMYKEL
     EQSVPGFTRP SHGYLESWAR QGVMLLNTVL TVQAGKAHSH ANLGWETFTD KVISLINEHR
     EGVVFLLWGS HAQKKGAIID RNRHHVLQAP HPSPLSAHRG FFGCGHFVKT NQYLESRGEA
     PIDWMPVLPE NAQ
//