ID   A7GL70_BACCN            Unreviewed;       477 AA.
AC   A7GL70;
DT   11-SEP-2007, integrated into UniProtKB/TrEMBL.
DT   11-SEP-2007, sequence version 1.
DT   02-SEP-2026, entry version 111.
DE   RecName: Full=Aspartate ammonia-lyase {ECO:0000256|ARBA:ARBA00016146, ECO:0000256|NCBIfam:TIGR00839};
DE            Short=Aspartase {ECO:0000256|RuleBase:RU362017};
DE            EC=4.3.1.1 {ECO:0000256|ARBA:ARBA00012992, ECO:0000256|NCBIfam:TIGR00839};
GN   OrderedLocusNames=Bcer98_0526 {ECO:0000313|EMBL:ABS20878.1};
OS   Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Bacillaceae;
OC   Bacillus; Bacillus cereus group.
OX   NCBI_TaxID=315749 {ECO:0000313|EMBL:ABS20878.1, ECO:0000313|Proteomes:UP000002300};
RN   [1] {ECO:0000313|EMBL:ABS20878.1, ECO:0000313|Proteomes:UP000002300}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22905 / CIP 110041 / 391-98 / NVH 391-98
RC   {ECO:0000313|Proteomes:UP000002300};
RX   PubMed=17434157; DOI=10.1016/j.cbi.2007.03.003;
RA   Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C.,
RA   Land M.L., Broussolle V., Brillard J., Guinebretiere M.H., Sanchis V.,
RA   Nguen-The C., Lereclus D., Richardson P., Wincker P., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "Extending the Bacillus cereus group genomics to putative food-borne
RT   pathogens of different toxicity.";
RL   Chem. Biol. Interact. 171:236-249(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-aspartate = fumarate + NH4(+); Xref=Rhea:RHEA:16601,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29806, ChEBI:CHEBI:29991; EC=4.3.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001494,
CC         ECO:0000256|RuleBase:RU362017};
CC   -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family.
CC       Aspartase subfamily. {ECO:0000256|ARBA:ARBA00005596,
CC       ECO:0000256|RuleBase:RU362017}.
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DR   EMBL; CP000764; ABS20878.1; -; Genomic_DNA.
DR   RefSeq; WP_011983634.1; NC_009674.1.
DR   AlphaFoldDB; A7GL70; -.
DR   STRING; 315749.Bcer98_0526; -.
DR   PaxDb; 315749-Bcer98_0526; -.
DR   GeneID; 33895885; -.
DR   KEGG; bcy:Bcer98_0526; -.
DR   eggNOG; COG1027; Bacteria.
DR   HOGENOM; CLU_021594_4_0_9; -.
DR   OrthoDB; 9802809at2; -.
DR   Proteomes; UP000002300; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0008797; F:aspartate ammonia-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006531; P:aspartate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   CDD; cd01357; Aspartase; 1.
DR   FunFam; 1.10.40.30:FF:000002; Fumarate hydratase class II; 1.
DR   FunFam; 1.10.275.10:FF:000001; Fumarate hydratase, mitochondrial; 1.
DR   FunFam; 1.20.200.10:FF:000001; Fumarate hydratase, mitochondrial; 1.
DR   Gene3D; 1.10.40.30; Fumarase/aspartase (C-terminal domain); 1.
DR   Gene3D; 1.20.200.10; Fumarase/aspartase (Central domain); 1.
DR   Gene3D; 1.10.275.10; Fumarase/aspartase (N-terminal domain); 1.
DR   InterPro; IPR004708; ApsA.
DR   InterPro; IPR051546; Aspartate_Ammonia-Lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR018951; Fumarase_C_C.
DR   InterPro; IPR020557; Fumarate_lyase_CS.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   NCBIfam; TIGR00839; aspA; 1.
DR   NCBIfam; NF008909; PRK12273.1; 1.
DR   NCBIfam; NF011092; PRK14515.1; 1.
DR   PANTHER; PTHR42696; ASPARTATE AMMONIA-LYASE; 1.
DR   PANTHER; PTHR42696:SF2; ASPARTATE AMMONIA-LYASE; 1.
DR   Pfam; PF10415; FumaraseC_C; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00145; ARGSUCLYASE.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SUPFAM; SSF48557; L-aspartase-like; 1.
DR   PROSITE; PS00163; FUMARATE_LYASES; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU362017};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002300}.
FT   DOMAIN          15..344
FT                   /note="Fumarate lyase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00206"
FT   DOMAIN          410..463
FT                   /note="Fumarase C C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF10415"
SQ   SEQUENCE   477 AA;  52624 MW;  3E65C8ADA5CD0852 CRC64;
     MIATKDIRIE KDFLGEKEVP NEAYYGVQTL RAVENFPITG YRIHDSLITA MAIVKKAAAL
     ANVETGYLPE NIGKEIAAAA QEIMDGKLHD QFIVDPIQGG AGTSINMNAN EVIANRALER
     MGYEKGDYAK ISPNTHVNMA QSTNDAFPTG IHIATLMMLE ELLITMEELH RAFIKKAKEF
     DHVIKMGRTH LQDAVPIRLG QEFSAYSRVL ERDIKRIKQS RQHLYEVNMG ATAVGTGLNA
     NPMYIEQVVK HLRELSGFPL VGAEHLVDAT QNTDAYTEVS AALKVCMMNM SKIANDLRIM
     ASGPRVGLAE IQLPARQPGS SIMPGKVNPV MAEVINQVAF QVIGNDHTIC LASEAGQLEL
     NVMEPVLVFN LIQSISIMNN AFRVFREYCI EGITANEELL KEYVERSVGI ITAVNPHIGY
     EAASRIAREA IATGKSVREL CLEHGVLTEE ELDIILDPYE MTHPEIAGAS LLKNKKS
//