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<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-09-02" version="94" xmlns="http://uniprot.org/uniprot">
  <accession>A6T9H1</accession>
  <name>PQQC_KLEP7</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">Pyrroloquinoline-quinone synthase</fullName>
      <ecNumber evidence="1">1.3.3.11</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">Coenzyme PQQ synthesis protein C</fullName>
    </alternativeName>
    <alternativeName>
      <fullName evidence="1">Pyrroloquinoline quinone biosynthesis protein C</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">pqqC</name>
    <name type="ordered locus">KPN78578_17810</name>
    <name type="ORF">KPN_01811</name>
  </gene>
  <organism>
    <name type="scientific">Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)</name>
    <dbReference type="NCBI Taxonomy" id="272620"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Enterobacterales</taxon>
      <taxon>Enterobacteriaceae</taxon>
      <taxon>Klebsiella</taxon>
      <taxon>Klebsiella pneumoniae complex</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="submission" date="2006-09" db="EMBL/GenBank/DDBJ databases">
      <authorList>
        <consortium name="The Klebsiella pneumonia Genome Sequencing Project"/>
        <person name="McClelland M."/>
        <person name="Sanderson E.K."/>
        <person name="Spieth J."/>
        <person name="Clifton W.S."/>
        <person name="Latreille P."/>
        <person name="Sabo A."/>
        <person name="Pepin K."/>
        <person name="Bhonagiri V."/>
        <person name="Porwollik S."/>
        <person name="Ali J."/>
        <person name="Wilson R.K."/>
      </authorList>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>ATCC 700721 / MGH 78578</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 = pyrroloquinoline quinone + 2 H2O2 + 2 H2O + H(+)</text>
      <dbReference type="Rhea" id="RHEA:10692"/>
      <dbReference type="ChEBI" id="CHEBI:15377"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:15379"/>
      <dbReference type="ChEBI" id="CHEBI:16240"/>
      <dbReference type="ChEBI" id="CHEBI:58442"/>
      <dbReference type="ChEBI" id="CHEBI:58778"/>
      <dbReference type="EC" id="1.3.3.11"/>
    </reaction>
  </comment>
  <comment type="pathway">
    <text evidence="1">Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.</text>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the PqqC family.</text>
  </comment>
  <dbReference type="EC" id="1.3.3.11" evidence="1"/>
  <dbReference type="EMBL" id="CP000647">
    <property type="protein sequence ID" value="ABR77242.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_015958460.1">
    <property type="nucleotide sequence ID" value="NC_009648.1"/>
  </dbReference>
  <dbReference type="PDB" id="3HLX">
    <property type="method" value="X-ray"/>
    <property type="resolution" value="1.30 A"/>
    <property type="chains" value="A/D=1-251"/>
  </dbReference>
  <dbReference type="PDB" id="3HML">
    <property type="method" value="X-ray"/>
    <property type="resolution" value="2.35 A"/>
    <property type="chains" value="A/B=1-251"/>
  </dbReference>
  <dbReference type="PDB" id="3HNH">
    <property type="method" value="X-ray"/>
    <property type="resolution" value="1.80 A"/>
    <property type="chains" value="A=1-251"/>
  </dbReference>
  <dbReference type="PDB" id="4NY7">
    <property type="method" value="X-ray"/>
    <property type="resolution" value="1.44 A"/>
    <property type="chains" value="A/B=1-251"/>
  </dbReference>
  <dbReference type="PDBsum" id="3HLX"/>
  <dbReference type="PDBsum" id="3HML"/>
  <dbReference type="PDBsum" id="3HNH"/>
  <dbReference type="PDBsum" id="4NY7"/>
  <dbReference type="AlphaFoldDB" id="A6T9H1"/>
  <dbReference type="SMR" id="A6T9H1"/>
  <dbReference type="STRING" id="272620.KPN_01811"/>
  <dbReference type="PaxDb" id="272620-KPN_01811"/>
  <dbReference type="EnsemblBacteria" id="ABR77242">
    <property type="protein sequence ID" value="ABR77242"/>
    <property type="gene ID" value="KPN_01811"/>
  </dbReference>
  <dbReference type="KEGG" id="kpn:KPN_01811"/>
  <dbReference type="HOGENOM" id="CLU_080136_0_0_6"/>
  <dbReference type="BRENDA" id="1.3.3.11">
    <property type="organism ID" value="2814"/>
  </dbReference>
  <dbReference type="UniPathway" id="UPA00539"/>
  <dbReference type="EvolutionaryTrace" id="A6T9H1"/>
  <dbReference type="Proteomes" id="UP000000265">
    <property type="component" value="Chromosome"/>
  </dbReference>
  <dbReference type="GO" id="GO:0033732">
    <property type="term" value="F:pyrroloquinoline-quinone synthase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-EC"/>
  </dbReference>
  <dbReference type="GO" id="GO:0018189">
    <property type="term" value="P:pyrroloquinoline quinone biosynthetic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="CDD" id="cd19370">
    <property type="entry name" value="TenA_PqqC"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="1.20.910.10">
    <property type="entry name" value="Heme oxygenase-like"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_00654">
    <property type="entry name" value="PQQ_syn_PqqC"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR016084">
    <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR011845">
    <property type="entry name" value="PqqC"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR039068">
    <property type="entry name" value="PqqC-like"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR004305">
    <property type="entry name" value="Thiaminase-2/PQQC"/>
  </dbReference>
  <dbReference type="NCBIfam" id="TIGR02111">
    <property type="entry name" value="PQQ_syn_pqqC"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR40279:SF3">
    <property type="entry name" value="4-AMINOBENZOATE SYNTHASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR40279">
    <property type="entry name" value="PQQC-LIKE PROTEIN"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF03070">
    <property type="entry name" value="TENA_THI-4"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF48613">
    <property type="entry name" value="Heme oxygenase-like"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="evidence at protein level"/>
  <keyword id="KW-0002">3D-structure</keyword>
  <keyword id="KW-0560">Oxidoreductase</keyword>
  <keyword id="KW-0884">PQQ biosynthesis</keyword>
  <keyword id="KW-1185">Reference proteome</keyword>
  <feature type="chain" id="PRO_1000061670" description="Pyrroloquinoline-quinone synthase">
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    </location>
  </feature>
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  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_00654"/>
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  </evidence>
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    </source>
  </evidence>
  <evidence type="ECO:0007829" key="3">
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      <dbReference type="PDB" id="3HML"/>
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  </evidence>
  <sequence length="251" mass="29030" checksum="62122408784C8EB3" modified="2007-08-21" version="1">MLITDTLSPQAFEEALRAKGDFYHIHHPYHIAMHNGNATREQIQGWVANRFYYQTTIPLKDAAIMANCPDAQTRRKWVQRILDHDGSHGEDGGIEAWLRLGEAVGLSRDDLLSERHVLPGVRFAVDAYLNFARRACWQEAACSSLTELFAPQIHQSRLDSWPQHYPWIKEEGYFYFRSRLSQANRDVEHGLALAKAYCDSAEKQNRMLEILQFKLDILWSMLDAMTMAYALQRPPYHTVTDKAAWHTTRLV</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>