ID   UB2L6_BOVIN             Reviewed;         153 AA.
AC   A5PJC4;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   02-SEP-2026, entry version 109.
DE   RecName: Full=Ubiquitin/ISG15-conjugating enzyme E2 L6;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme L6;
DE   AltName: Full=Ubiquitin carrier protein L6;
DE   AltName: Full=Ubiquitin-protein ligase L6;
GN   Name=UBE2L6;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
CC       Promotes ubiquitination and subsequent proteasomal degradation of FLT3.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC         ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SUBUNIT: Interacts with RNF19A, RNF19B and RNF144B. Interacts with FLT3
CC       (tyrosine phosphorylated). {ECO:0000250|UniProtKB:O14933}.
CC   -!- PTM: ISGylated. {ECO:0000250|UniProtKB:O14933}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; BC142048; AAI42049.1; -; mRNA.
DR   RefSeq; NP_001092387.1; NM_001098917.1.
DR   PDB; 9JTC; EM; 2.99 A; B=1-153.
DR   AlphaFoldDB; A5PJC4; -.
DR   EMDB; EMD-61794; -.
DR   SMR; A5PJC4; -.
DR   FunCoup; A5PJC4; 296.
DR   STRING; 9913.ENSBTAP00000017267; -.
DR   PaxDb; 9913-ENSBTAP00000017267; -.
DR   GeneID; 509471; -.
DR   KEGG; bta:509471; -.
DR   CTD; 9246; -.
DR   VGNC; VGNC:36588; UBE2L6.
DR   eggNOG; KOG0422; Eukaryota.
DR   HOGENOM; CLU_030988_13_3_1; -.
DR   InParanoid; A5PJC4; -.
DR   OrthoDB; 9973183at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0042296; F:ISG15 transferase activity; IBA:GO_Central.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0032020; P:ISG15-protein conjugation; IBA:GO_Central.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd23801; UBCc_UBE2L3; 1.
DR   FunFam; 3.10.110.10:FF:000011; Ubiquitin-conjugating enzyme E2 L3; 1.
DR   Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR   InterPro; IPR050113; Ub_conjugating_enzyme-E2-like.
DR   InterPro; IPR000608; UBC.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   PANTHER; PTHR24067; UBIQUITIN-CONJUGATING ENZYME E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   SMART; SM00212; UBCc; 1.
DR   SUPFAM; SSF54495; UBC-like; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
DR   PDBsum; 9JTC; -.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Transferase; Ubl conjugation;
KW   Ubl conjugation pathway.
FT   CHAIN           1..153
FT                   /note="Ubiquitin/ISG15-conjugating enzyme E2 L6"
FT                   /id="PRO_0000375220"
FT   DOMAIN          2..149
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        86
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
FT   HELIX           4..15
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   STRAND          22..27
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   STRAND          34..39
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   HELIX           46..48
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   STRAND          49..56
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   TURN            59..63
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   STRAND          67..72
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   STRAND          77..79
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   TURN            92..94
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   HELIX           101..113
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   HELIX           123..131
FT                   /evidence="ECO:0007829|PDB:9JTC"
FT   HELIX           133..146
FT                   /evidence="ECO:0007829|PDB:9JTC"
SQ   SEQUENCE   153 AA;  17825 MW;  9FE5AD0B75E81BC4 CRC64;
     MTASKRVAKE LEDLQKELPK YLRNLCSEED NVLVWHALLL PERPPYNLKA FRLCISFPRE
     YPFQPPTVKF TTRIYHPNVD RDGRVCLPII SKKNWKASTR TCQVLEALNM LVNQPEPGQP
     VRLELAEQLT QDPELFDQMA QEFTLQFGVD RPS
//