ID   A5FGA0_FLAJ1            Unreviewed;       799 AA.
AC   A5FGA0;
DT   12-JUN-2007, integrated into UniProtKB/TrEMBL.
DT   12-JUN-2007, sequence version 1.
DT   02-SEP-2026, entry version 95.
DE   RecName: Full=Glucan 1,4-alpha-glucosidase {ECO:0000256|PROSITE-ProRule:PRU10066};
DE            EC=3.2.1.3 {ECO:0000256|PROSITE-ProRule:PRU10066};
GN   OrderedLocusNames=Fjoh_2746 {ECO:0000313|EMBL:ABQ05768.1};
OS   Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / BCRC
OS   14874 / CCUG 350202 / NBRC 14942 / NCIMB 11054 / UW101) (Cytophaga
OS   johnsonae).
OC   Bacteria; Pseudomonadati; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Flavobacterium.
OX   NCBI_TaxID=376686 {ECO:0000313|EMBL:ABQ05768.1, ECO:0000313|Proteomes:UP000006694};
RN   [1] {ECO:0000313|EMBL:ABQ05768.1, ECO:0000313|Proteomes:UP000006694}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17061 / DSM 2064 / JCM 8514 / BCRC 14874 / CCUG 350202 /
RC   NBRC 14942 / NCIMB 11054 / UW101 {ECO:0000313|Proteomes:UP000006694};
RX   PubMed=19717629; DOI=10.1128/AEM.01495-09;
RA   McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G.,
RA   Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R.,
RA   Cheng Y.Q., Stein J.L.;
RT   "Novel features of the polysaccharide-digesting gliding bacterium
RT   Flavobacterium johnsoniae as revealed by genome sequence analysis.";
RL   Appl. Environ. Microbiol. 75:6864-6875(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues
CC         successively from non-reducing ends of the chains with release of
CC         beta-D-glucose.; EC=3.2.1.3; Evidence={ECO:0000256|PROSITE-
CC         ProRule:PRU10066};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family.
CC       {ECO:0000256|ARBA:ARBA00007806, ECO:0000256|RuleBase:RU361185}.
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DR   EMBL; CP000685; ABQ05768.1; -; Genomic_DNA.
DR   RefSeq; WP_012024807.1; NC_009441.1.
DR   AlphaFoldDB; A5FGA0; -.
DR   STRING; 376686.Fjoh_2746; -.
DR   GeneID; 31765660; -.
DR   KEGG; fjo:Fjoh_2746; -.
DR   eggNOG; COG1501; Bacteria.
DR   HOGENOM; CLU_000631_7_2_10; -.
DR   OrthoDB; 176168at2; -.
DR   Proteomes; UP000006694; Chromosome.
DR   GO; GO:0004558; F:alpha-1,4-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd06604; GH31_glucosidase_II_MalA; 1.
DR   CDD; cd14752; GH31_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1760; glycosyl hydrolase (family 31); 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR   InterPro; IPR033403; DUF5110.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR030458; Glyco_hydro_31_AS.
DR   InterPro; IPR048395; Glyco_hydro_31_C.
DR   InterPro; IPR025887; Glyco_hydro_31_N_dom.
DR   InterPro; IPR000322; Glyco_hydro_31_TIM.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   PANTHER; PTHR22762; ALPHA-GLUCOSIDASE; 1.
DR   PANTHER; PTHR22762:SF120; HETEROGLYCAN GLUCOSIDASE 1; 1.
DR   Pfam; PF17137; DUF5110; 1.
DR   Pfam; PF13802; Gal_mutarotas_2; 1.
DR   Pfam; PF01055; Glyco_hydro_31_2nd; 1.
DR   Pfam; PF21365; Glyco_hydro_31_3rd; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR   PROSITE; PS00129; GLYCOSYL_HYDROL_F31_1; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361185};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361185};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006694}.
FT   DOMAIN          38..209
FT                   /note="Glycoside hydrolase family 31 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF13802"
FT   DOMAIN          251..579
FT                   /note="Glycoside hydrolase family 31 TIM barrel"
FT                   /evidence="ECO:0000259|Pfam:PF01055"
FT   DOMAIN          587..673
FT                   /note="Glycosyl hydrolase family 31 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF21365"
FT   DOMAIN          689..757
FT                   /note="DUF5110"
FT                   /evidence="ECO:0000259|Pfam:PF17137"
FT   ACT_SITE        405
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10066"
SQ   SEQUENCE   799 AA;  94067 MW;  F7A9769323B07CDA CRC64;
     MITNTSLEYK GDLYPSKIVS YEHEGDSIFF NTDNKVILKV TILRDSLIRF RFTTKGYFSN
     DFSYAIDKTQ LHGYNFLELT EQETYFEIRT SKVKCKIQKA DLRLSIYDLN DFLILEDELG
     FHWEESYEYG GNIVKMSKFS KDGECYYGLG DKATQMNLKG KRVENFATDQ YAYQKDQEPL
     YKVVPFYIGL HNKQSYGIFF DNTFRTFFDF CQERRNITSF WAEGGEMNYY FIYGPQMQDV
     VTTYTDLTGK PELPPLWVLG YHQCKWSYYP ESKVKEITSK FRELKIPCDA IYLDIDYMDG
     FRCFTWNKNY FPDPKKMVTE LAEDGFKTVV IIDPGIKIDK DYWVYKEALE KDYFCKRADG
     PYMKGKVWPG ECNFPDYTNP AVREWWAGLF KELVSEIGVK GVWNDMNEPA VMEVPNKTFP
     MDVRHFYEGN PCSHRKAHNI YGTQMARATY HGVKRFTYPK RPFVITRSAY SGAQRYTSSW
     TGDNVATWEH LWIANIQVQR MSISGMGFTG SDIGGFAEQP TGELYARWIQ LGVFHPFCRT
     HSSGDHGNQE PWAFDEEVIT ITRKFVSLRY QLLPYLYTMF WQYIEEGVPM LKPLVYYDQD
     DTQTHYRNDE FIFGNQILVC PILEPNAVGR RMYIPRGEWY SYWTNELFIG GREIWIDTKF
     DEIPVFVKAG AIIPKYPVQQ YVGELEFDEL TLDVYYKNGK EQSAVYEDAQ DGYDYKKGRY
     SYLSLRNIGK EKELIIQLHK EGKYTTPYTK YKINLIGLPF KVTEIEIDNE KIEFDKVSFE
     LNNFLIVDKE FNELHIVGE
//