ID   A4XM78_CALS8            Unreviewed;       321 AA.
AC   A4XM78;
DT   29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2007, sequence version 1.
DT   28-JAN-2026, entry version 76.
DE   SubName: Full=Acetyl xylan esterase {ECO:0000313|EMBL:ABP68013.1};
GN   OrderedLocusNames=Csac_2436 {ECO:0000313|EMBL:ABP68013.1};
OS   Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T
OS   6331).
OC   Bacteria; Bacillati; Bacillota; Bacillota incertae sedis;
OC   Caldicellulosiruptorales; Caldicellulosiruptoraceae; Caldicellulosiruptor.
OX   NCBI_TaxID=351627 {ECO:0000313|EMBL:ABP68013.1, ECO:0000313|Proteomes:UP000000256};
RN   [1] {ECO:0000313|EMBL:ABP68013.1, ECO:0000313|Proteomes:UP000000256}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43494 / DSM 8903 / Tp8T 6331
RC   {ECO:0000313|Proteomes:UP000000256};
RX   PubMed=18776029; DOI=10.1128/AEM.00968-08;
RA   van de Werken H.J., Verhaart M.R., VanFossen A.L., Willquist K.,
RA   Lewis D.L., Nichols J.D., Goorissen H.P., Mongodin E.F., Nelson K.E.,
RA   van Niel E.W., Stams A.J., Ward D.E., de Vos W.M., van der Oost J.,
RA   Kelly R.M., Kengen S.W.;
RT   "Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor
RT   saccharolyticus.";
RL   Appl. Environ. Microbiol. 74:6720-6729(2008).
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DR   EMBL; CP000679; ABP68013.1; -; Genomic_DNA.
DR   RefSeq; WP_011917935.1; NC_009437.1.
DR   AlphaFoldDB; A4XM78; -.
DR   STRING; 351627.Csac_2436; -.
DR   ESTHER; cals8-a4xm78; Acetyl-esterase_deacetylase.
DR   KEGG; csc:Csac_2436; -.
DR   eggNOG; COG3458; Bacteria.
DR   HOGENOM; CLU_054209_0_0_9; -.
DR   OrthoDB; 9770528at2; -.
DR   Proteomes; UP000000256; Chromosome.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:TreeGrafter.
DR   GO; GO:0005976; P:polysaccharide metabolic process; IEA:TreeGrafter.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase_fold.
DR   InterPro; IPR008391; AXE1_dom.
DR   InterPro; IPR039069; CE7.
DR   PANTHER; PTHR40111; CEPHALOSPORIN-C DEACETYLASE; 1.
DR   PANTHER; PTHR40111:SF1; CEPHALOSPORIN-C DEACETYLASE; 1.
DR   Pfam; PF05448; AXE1; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000000256}.
FT   DOMAIN          2..318
FT                   /note="Acetyl xylan esterase"
FT                   /evidence="ECO:0000259|Pfam:PF05448"
FT   ACT_SITE        183
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639069-1"
FT   ACT_SITE        273
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639069-1"
FT   ACT_SITE        302
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639069-1"
FT   BINDING         92
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639069-2"
SQ   SEQUENCE   321 AA;  36577 MW;  9122D6E5F358DC8A CRC64;
     MVFDMPLDQL KTYMGTNPCP PDFDEYWQKA LKEMDDVDPQ VEIVKETSIE APYAECYNLY
     FTGVKGARIR SQLIKPKKIE KPCPAVLSFH GYAWYSGDWS DKFALAAAGF VVAAMDVRGQ
     NGYSEDVGGV KGNTVHGHII RGLDGDKEDL LYRQIFLDTA ELAKIIANMP DVDSSKIAAM
     GFSQGGGLAL ACGALSPYVS RVISVYPFLC DYKRVWEMDL AKDAYEEIRT YFRFRDPLHE
     REDEIFTKLG YIDVQHLAKW IKAEVLMVTG LMDTICPPST QFAAYNKIQS KKQMLIYPDF
     GHEQIFYLND RIFMYLMEML E
//