ID   A4XM50_CALS8            Unreviewed;       337 AA.
AC   A4XM50;
DT   29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2007, sequence version 1.
DT   02-SEP-2026, entry version 82.
DE   RecName: Full=Beta-xylanase {ECO:0000256|RuleBase:RU361174};
DE            EC=3.2.1.8 {ECO:0000256|RuleBase:RU361174};
GN   OrderedLocusNames=Csac_2408 {ECO:0000313|EMBL:ABP67985.1};
OS   Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T
OS   6331).
OC   Bacteria; Bacillati; Bacillota; Bacillota incertae sedis;
OC   Caldicellulosiruptorales; Caldicellulosiruptoraceae; Caldicellulosiruptor.
OX   NCBI_TaxID=351627 {ECO:0000313|EMBL:ABP67985.1, ECO:0000313|Proteomes:UP000000256};
RN   [1] {ECO:0000313|EMBL:ABP67985.1, ECO:0000313|Proteomes:UP000000256}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43494 / DSM 8903 / Tp8T 6331
RC   {ECO:0000313|Proteomes:UP000000256};
RX   PubMed=18776029; DOI=10.1128/AEM.00968-08;
RA   van de Werken H.J., Verhaart M.R., VanFossen A.L., Willquist K.,
RA   Lewis D.L., Nichols J.D., Goorissen H.P., Mongodin E.F., Nelson K.E.,
RA   van Niel E.W., Stams A.J., Ward D.E., de Vos W.M., van der Oost J.,
RA   Kelly R.M., Kengen S.W.;
RT   "Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor
RT   saccharolyticus.";
RL   Appl. Environ. Microbiol. 74:6720-6729(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.;
CC         EC=3.2.1.8; Evidence={ECO:0000256|PROSITE-ProRule:PRU01096,
CC         ECO:0000256|RuleBase:RU361174};
CC   -!- PATHWAY: Glycan degradation; xylan degradation. {ECO:0000256|PROSITE-
CC       ProRule:PRU01096}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 10 (cellulase F) family.
CC       {ECO:0000256|PROSITE-ProRule:PRU01096, ECO:0000256|RuleBase:RU361174}.
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DR   EMBL; CP000679; ABP67985.1; -; Genomic_DNA.
DR   AlphaFoldDB; A4XM50; -.
DR   SMR; A4XM50; -.
DR   STRING; 351627.Csac_2408; -.
DR   CAZy; GH10; Glycoside Hydrolase Family 10.
DR   KEGG; csc:Csac_2408; -.
DR   eggNOG; COG3693; Bacteria.
DR   HOGENOM; CLU_020161_6_1_9; -.
DR   Proteomes; UP000000256; Chromosome.
DR   GO; GO:0031176; F:endo-1,4-beta-xylanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR044846; GH10.
DR   InterPro; IPR031158; GH10_AS.
DR   InterPro; IPR001000; GH10_dom.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   PANTHER; PTHR31490:SF90; ENDO-1,4-BETA-XYLANASE A; 1.
DR   PANTHER; PTHR31490; GLYCOSYL HYDROLASE; 1.
DR   Pfam; PF00331; Glyco_hydro_10; 1.
DR   PRINTS; PR00134; GLHYDRLASE10.
DR   SMART; SM00633; Glyco_10; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00591; GH10_1; 1.
DR   PROSITE; PS51760; GH10_2; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU01096};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW   ProRule:PRU01096};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01096};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU01096};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000256};
KW   Xylan degradation {ECO:0000256|PROSITE-ProRule:PRU01096}.
FT   DOMAIN          6..337
FT                   /note="GH10"
FT                   /evidence="ECO:0000259|PROSITE:PS51760"
FT   ACT_SITE        139
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01096"
FT   ACT_SITE        247
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10061"
SQ   SEQUENCE   337 AA;  39871 MW;  B266A1B3740CC108 CRC64;
     MCENLEMLNL SLAKTYKDYF KIGAAVTAKD LEGVHRDILL KHFNSLTPEN AMKFENIHPE
     EQRYNFEEVA RIKEFAIKND MKLRGHTFVW HNQTPGWVFL DKNGEEASKE LVIERLREHI
     KTLCERYKDV VYAWDVVNEA VEDKTEKLLR ESNWRKIIGD DYIKIAFEIA REYAGDAKLF
     YNDYNNEMPY KLEKTYKVLK ELLERGTPID GIGIQAHWNI WDKNLVSNLK KAIEVYASLG
     LEIHITELDI SVFEFEDKRT DLFEPTPEML ELQAKVYEDV FAVFREYKDV ITSVTLWGIS
     DRHTWKDNFP VKGRKDWPLL FDVNGKPKEA LYRILRF
//