ID   A4VLE4_STUS1            Unreviewed;       851 AA.
AC   A4VLE4;
DT   29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2007, sequence version 1.
DT   02-SEP-2026, entry version 93.
DE   RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE            EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE   AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN   OrderedLocusNames=PST_2130 {ECO:0000313|EMBL:ABP79795.1};
OS   Stutzerimonas stutzeri (strain A1501) (Pseudomonas stutzeri).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pseudomonadales; Pseudomonadaceae; Stutzerimonas.
OX   NCBI_TaxID=379731 {ECO:0000313|EMBL:ABP79795.1, ECO:0000313|Proteomes:UP000000233};
RN   [1] {ECO:0000313|EMBL:ABP79795.1, ECO:0000313|Proteomes:UP000000233}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A1501 {ECO:0000313|EMBL:ABP79795.1,
RC   ECO:0000313|Proteomes:UP000000233};
RX   PubMed=18495935; DOI=10.1073/pnas.0801093105;
RA   Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W.,
RA   Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y.,
RA   Li D., Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT   "Nitrogen fixation island and rhizosphere competence traits in the genome
RT   of root-associated Pseudomonas stutzeri A1501.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7564-7569(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC         diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
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DR   EMBL; CP000304; ABP79795.1; -; Genomic_DNA.
DR   RefSeq; WP_011913262.1; NC_009434.1.
DR   AlphaFoldDB; A4VLE4; -.
DR   KEGG; psa:PST_2130; -.
DR   eggNOG; COG1793; Bacteria.
DR   eggNOG; COG3285; Bacteria.
DR   HOGENOM; CLU_008325_0_0_6; -.
DR   Proteomes; UP000000233; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR   CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR   CDD; cd07971; OBF_DNA_ligase_LigD; 1.
DR   CDD; cd04862; PaeLigD_Pol_like; 1.
DR   Gene3D; 3.30.1490.70; -; 1.
DR   Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR   Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR014146; LigD_ligase_dom.
DR   InterPro; IPR014144; LigD_PE_domain.
DR   InterPro; IPR014145; LigD_pol_dom.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR014143; NHEJ_ligase_prk.
DR   InterPro; IPR052171; NHEJ_LigD.
DR   InterPro; IPR033651; PaeLigD_Pol-like.
DR   NCBIfam; TIGR02777; LigD_PE_dom; 1.
DR   NCBIfam; TIGR02778; ligD_pol; 1.
DR   NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR   NCBIfam; TIGR02776; NHEJ_ligase_prk; 1.
DR   NCBIfam; NF004628; PRK05972.1; 1.
DR   PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR   PANTHER; PTHR42705:SF2; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF13298; LigD_N; 1.
DR   Pfam; PF21686; LigD_Prim-Pol; 1.
DR   SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW   DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW   DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:ABP79795.1};
KW   Manganese {ECO:0000256|ARBA:ARBA00023211};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000233};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          323..415
FT                   /note="ATP-dependent DNA ligase family profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50160"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          189..213
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..11
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   851 AA;  94475 MW;  B7863640D08E647E CRC64;
     MALEDYQRMR DFAATPEPAG KARPKPRKSQ ALQYCIQKHD ATRLHYDFRL ELDGTLKSWA
     IPKGPSLDPG VRRLAVHVED HPLEYATFEG NIPEGHYGAG DVIVWDRGVW TPQGDPQEGY
     RKGKLKFSLE GEKLAGSWNL VRTQMDGKKE QWFLIKSRDE AAREESEYDI VTAEPDSVLS
     DRTLVPRKRG AKARVAETPP APVKAPAKKR TPARRKAKVA LEGAVPAELP ERFKPQLATL
     VDTVPVGDWR YEIKFDGYRM LARIDAGEVR LFTRNGHDWT AKMPQQAAAL AGLGLESGWL
     DGEVVVPNEE GTPDFQALQN AFEAGRSGSI LYYLFDMPYL NGMDLREVAL EQRRAALREV
     LERSDSELLR FSEDFTEQPD SILESACQMK LEGLIGKRAG STYTSRRSSS WVKIKCSNRQ
     EFIIVGYTQP KGTRTGFGAL LLGLHDDKGE LRYAGKVGTG FNHATLQSLH KKLKKLETDK
     SPLAKAPPAA DARGAQWLKP ELMCEVAYAE MTRQGVVRHS VFHGLRTDKP AEAITHERAK
     PVARATPNVA GKADPLGSGR IKISNPERVI DPSSGITKIE LARFYAQIAP WALPQLQNRP
     LALVRAPEGI TGELFFQKHA DKLAIPHITQ LDPALDPKHG ALLVIDSAEA LVGAAQMGTI
     ELHSWNAVAP DLEHPDRFVL DLDPDPALPW KRMLEATQLT QTLLDEIGLA SFLKTSGGKG
     LHIVVPLDPV QDWSEVKSFS QAIARHLATL LPSHFSAVSG PKNRVGRIFI DYLRNSRGAS
     TVAPYSVRAR EGLPVSVPIH RDELADLKGA NLWTVRNLLE RLQELGDDDP WAAIGKTRQR
     LTKKIRDRLN A
//